Spp1 (NM_012881) Rat Tagged ORF Clone

CAT#: RR208248

Spp1 (Myc-DDK-tagged ORF) - Rat secreted phosphoprotein 1 (Spp1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Spp1
• Synonyms: OSP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR208248 representing NM_012881
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGACTGGCAGTGGTTTGCTTTTGCCTGTTCGGCCTTGCCTCCTGTCTCCCGGTGAAAGTGGCTGAGT
  TTGGCAGCTCAGAGGAGAAGGCGCATTACAGCAAACACTCAGATGCTGTAGCCACTTGGCTGAAGCCTGA
  CCCATCTCAGAAGCAGAATCTTCTAGCCCCACAGAATTCTGTGTCCTCTGAAGAAACGGATGACTTTAAG
  CAAGAAACTCTTCCAAGCAACTCCAATGAAAGCCATGACCACATGGACGATGATGACGACGACGATGACG
  ACGGAGACCATGCAGAGAGCGAGGATTCTGTGAACTCGGATGAATCTGACGAATCTCACCATTCCGATGA
  ATCTGATGAGTCCTTCACTGCCAGCACACAAGCAGACGTTTTGACTCCAATCGCCCCCACAGTCGATGTC
  CCTGACGGCCGAGGTGATAGCTTGGCTTACGGACTGAGGTCAAAGTCCAGGAGTTTCCCTGTTTCTGATG
  AACAGTATCCCGATGCCACAGATGAGGACCTCACCTCCCGCATGAAGAGCCAGGAGTCCGATGAGGCTAT
  CAAGGTCATCCCAGTTGCCCAGCGTCTGAGCGTGCCCTCTGATCAGGACAGCAACGGGAAGACCAGCCAT
  GAGTCAAGTCAGCTGGATGAACCAAGCGTGGAAACACACAGCCTGGAGCAGTCCAAGGAGTATAAGCAGA
  GGGCCAGCCACGAGAGCACTGAGCAGTCGGATGCGATCGATAGTGCCGAGAAGCCGGATGCAATCGATAG
  TGCGGAGCGGTCGGATGCTATCGACAGTCAGGCGAGTTCCAAAGCCAGCCTGGAACATCAGAGCCACGAG
  TTTCACAGCCATGAGGACAAGCTAGTCCTAGACCCTAAGAGTAAGGAAGATGATAGGTATCTGAAATTCC
  GCATTTCTCATGAATTAGAGAGTTCATCTTCTGAGGTCAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR208248 representing NM_012881
  Red=Cloning site Green=Tags(s)
  
  MRLAVVCFCLFGLASCLPVKVAEFGSSEEKAHYSKHSDAVATWLKPDPSQKQNLLAPQNSVSSEETDDFK
  QETLPSNSNESHDHMDDDDDDDDDGDHAESEDSVNSDESDESHHSDESDESFTASTQADVLTPIAPTVDV
  PDGRGDSLAYGLRSKSRSFPVSDEQYPDATDEDLTSRMKSQESDEAIKVIPVAQRLSVPSDQDSNGKTSH
  ESSQLDEPSVETHSLEQSKEYKQRASHESTEQSDAIDSAEKPDAIDSAERSDAIDSQASSKASLEHQSHE
  FHSHEDKLVLDPKSKEDDRYLKFRISHELESSSSEVN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012881
• ORF Size: 951 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_012881.1, NM_012881.2, NP_037013.2
• RefSeq Size: 1503
• RefSeq ORF: 954
• Locus ID: 25353
• MW: 35 kDa
• Gene Summary: sialoprotein that binds osteosarcoma cells; may play a role in osteoclast cell function [RGD, Feb 2006]