Acta2 (NM_031004) Rat Tagged ORF Clone

CAT#: RR208325

Acta2 (Myc-DDK-tagged ORF) - Rat smooth muscle alpha-actin (Acta2), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Acta2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR208325 representing NM_031004
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGTGAAGAGGAAGACAGCACAGCTCTGGTGTGTGACAATGGCTCCGGGCTCTGTAAGGCGGGCTTTG
  CTGGTGATGATGCTCCCAGGGCTGTTTTCCCATCCATCGTGGGACGTCCCAGACACCAGGGAGTGATGGT
  TGGAATGGGCCAAAAGGACAGCTATGTGGGGGACGAAGCGCAGAGCAAGAGAGGGATCCTGACCCTGAAG
  TATCCGATAGAACACGGCATCATCACCAACTGGGACGACATGGAAAAGATCTGGCACCACTCCTTCTATA
  ACGAGCTTCGCGTGGCCCCAGAGGAGCATCCGACCTTGCTAACGGAGGCGCCGCTGAACCCTAAGGCCAA
  CCGGGAGAAAATGACCCAGATTATGTTTGAGACCTTCAATGTCCCTGCCATGTATGTGGCTATTCAGGCT
  GTGCTGTCCCTCTATGCTTCTGGACGTACAACTGGTATTGTGCTGGACTCTGGAGATGGCGTGACTCACA
  ACGTGCCTATCTATGAGGGCTATGCTCTGCCTCATGCCATCATGCGTCTGGACTTGGCTGGCCGAGATCT
  CACCGACTACCTCATGAAGATCCTGACTGAGCGTGGCTATTCCTTCGTGACTACTGCTGAGCGTGAGATC
  GTCCGTGACATCAAGGAGAAGCTGTGCTATGTCGCTCTGGACTTTGAAAATGAGATGGCCACTGCTGCTT
  CCTCTTCTTCCCTGGAGAAGAGCTACGAACTGCCTGATGGGCAGGTGATCACCATCGGGAATGAACGCTT
  CCGCTGCCCGGAGACCCTCTTCCAGCCATCTTTCATTGGAATGGAGTCGGCGGGCATCCACGAAACCACC
  TATAACAGCATCATGAAGTGTGATATTGACATCAGGAAGGACCTCTATGCTAACAACGTCCTCTCGGGGG
  GCACTACCATGTACCCAGGCATTGCTGACAGGATGCAGAAGGAGATCACAGCCCTCGCTCCCAGCACCAT
  GAAGATCAAGATTATTGCTCCTCCAGAACGCAAATATTCTGTCTGGATCGGCGGCTCCATCCTGGCTTCT
  CTATCTACCTTCCAGCAGATGTGGATCAGCAAACAGGAGTATGACGAAGCTGGCCCCTCCATTGTCCACC
  GCAAATGCTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR208325 representing NM_031004
  Red=Cloning site Green=Tags(s)
  
  MCEEEDSTALVCDNGSGLCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLK
  YPIEHGIITNWDDMEKIWHHSFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQA
  VLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREI
  VRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETT
  YNSIMKCDIDIRKDLYANNVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILAS
  LSTFQQMWISKQEYDEAGPSIVHRKCF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031004
• ORF Size: 1131 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_031004.1, NM_031004.2, NP_112266.1
• RefSeq Size: 1412
• RefSeq ORF: 1134
• Locus ID: 81633
• MW: 42 kDa
• Gene Summary: contractile protein; required for extracellular matrix remodeling [RGD, Feb 2006]