Cyp7a1 (NM_012942) Rat Tagged ORF Clone

CAT#: RR208359

Cyp7a1 (Myc-DDK-tagged ORF) - Rat cytochrome P450, family 7, subfamily a, polypeptide 1 (Cyp7a1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cyp7a1
• Synonyms: CHAP; CYP7; CYP7S1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR208359 representing NM_012942
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGACTATTTCTTTGATTTGGGGAATTGCCGTGTTGGTGAGCTGTTGCATATGGTTTATTGTTGGAA
  TAAGGAGAAGGAAAGCTGGTGAACCTCCTTTGGAGAACGGGTTGATTCCGTACCTGGGCTGTGCTCTGAA
  ATTTGGATCTAATCCTCTTGAGTTCCTAAGAGCTAATCAAAGGAAGCATGGTCACGTTTTTACCTGCAAA
  CTGATGGGGAAATATGTCCATTTCATCACAAACTCCCTGTCATACCACAAAGTCTTATGTCATGGAAAAT
  ATTTTGACTGGAAAAAATTTCATTACACTACTTCTGCGAAGGCATTTGGACACAGAAGCATTGACCCAAA
  TGATGGAAATACCACGGAAAATATAAACAACACTTTTACCAAAACCCTCCAGGGAGATGCTCTGTGTTCA
  CTTTCTGAAGCCATGATGCAAAACCTCCAATCTGTCATGAGACCTCCTGGCCTTCCTAAATCAAAGAGCA
  ATGCCTGGGTCACGGAAGGGATGTATGCCTTCTGTTACCGAGTGATGTTTGAAGCTGGCTATCTAACACT
  GTTTGGCAGAGATATTTCAAAGACAGACACACAAAAAGCACTTATTCTAAACAACCTTGACAACTTCAAA
  CAATTTGACCAAGTCTTTCCGGCACTGGTGGCAGGCCTTCCTATTCACTTGTTCAAGACCGCACATAAAG
  CTCGGGAAAAGCTGGCTGAGGGATTGAAGCACAAGAACCTGTGTGTGAGGGACCAGGTCTCTGAACTGAT
  CCGTCTACGTATGTTTCTCAATGACACGCTCTCCACCTTTGACGACATGGAGAAGGCCAAGACGCACCTC
  GCTATTCTCTGGGCATCTCAAGCAAACACCATTCCTGCAACCTTTTGGAGCTTATTTCAAATGATCAGGA
  GTCCTGAAGCAATGAAAGCAGCCTCTGAAGAAGTGAGTGGAGCTTTACAGAGTGCTGGCCAAGAGCTCAG
  CTCTGGAGGGAGTGCCATTTACTTGGATCAAGTGCAACTGAATGACCTGCCGGTACTAGACAGCATCATC
  AAGGAGGCTCTGAGGCTTTCCAGTGCATCCTTGAATATCCGCACAGCTAAGGAGGACTTCACTCTCCATC
  TTGAGGACGGTTCCTATAACATCCGAAAAGATGACATGATAGCTCTTTATCCACAGTTAATGCACTTGGA
  TCCTGAAATCTACCCAGACCCTTTGACTTTCAAATATGACCGGTACCTTGATGAAAGCGGGAAAGCAAAG
  ACCACCTTCTACAGTAATGGAAACAAGCTGAAGTGTTTCTACATGCCCTTCGGATCAGGCGCGACAATAT
  GTCCTGGAAGACTCTTTGCCGTCCAAGAAATCAAGCAGTTTTTGATCCTGATGCTCTCCTGCTTTGAACT
  GGAGTTTGTGGAGAGCCAAGTCAAGTGTCCCCCTCTAGACCAGTCCCGGGCAGGCTTGGGAATTTTGCCA
  CCACTACATGATATTGAGTTTAAATATAAACTGAAACAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR208359 representing NM_012942
  Red=Cloning site Green=Tags(s)
  
  MMTISLIWGIAVLVSCCIWFIVGIRRRKAGEPPLENGLIPYLGCALKFGSNPLEFLRANQRKHGHVFTCK
  LMGKYVHFITNSLSYHKVLCHGKYFDWKKFHYTTSAKAFGHRSIDPNDGNTTENINNTFTKTLQGDALCS
  LSEAMMQNLQSVMRPPGLPKSKSNAWVTEGMYAFCYRVMFEAGYLTLFGRDISKTDTQKALILNNLDNFK
  QFDQVFPALVAGLPIHLFKTAHKAREKLAEGLKHKNLCVRDQVSELIRLRMFLNDTLSTFDDMEKAKTHL
  AILWASQANTIPATFWSLFQMIRSPEAMKAASEEVSGALQSAGQELSSGGSAIYLDQVQLNDLPVLDSII
  KEALRLSSASLNIRTAKEDFTLHLEDGSYNIRKDDMIALYPQLMHLDPEIYPDPLTFKYDRYLDESGKAK
  TTFYSNGNKLKCFYMPFGSGATICPGRLFAVQEIKQFLILMLSCFELEFVESQVKCPPLDQSRAGLGILP
  PLHDIEFKYKLKH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012942
• ORF Size: 1509 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_012942.1, NM_012942.2, NP_037074.1
• RefSeq Size: 3544
• RefSeq ORF: 1512
• Locus ID: 25428
• MW: 56.9 kDa
• Gene Summary: catalyzes the conversion of cholesterol, NADPH, and oxygen to 7-alpha-hydroxycholesterol, NADP(+) and water; plays a role in bile acid biosynthesis and cholesterol metabolism [RGD, Feb 2006]