Akt3 (NM_031575) Rat Tagged ORF Clone

CAT#: RR208375

Akt3 (Myc-DDK-tagged ORF) - Rat v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma) (Akt3), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Akt3
• Synonyms: Pkbg
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR208375 representing NM_031575
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGATGTTACCATCGTTAAAGAAGACTGGGTTCAGAAGAGGGGAGAATATATAAAAAATTGGAGGC
  CAAGATACTTCCTTTTGAAGACAGACGGCTCATTCATAGGCTATAAAGAGAAACCTCAAGATGTGGACTT
  ACCTTATCCCCTCAACAACTTCTCAGTGGCAAAATGTCAATTAATGAAAACAGAACGACCAAAGCCAAAT
  ACATTTATTATCAGATGTCTTCAGTGGACCACTGTTATAGAGAGAACATTTCATGTAGATACTCCAGAGG
  AAAGAGAAGAATGGACAGAAGCTATCCAAGCTGTAGCCGATCGACTGCAGAGGCAAGAAGAGGAGAGGAT
  GAATTGTAGTCCAACGTCACAGATTGATAATATTGGAGAGGAAGAGATGGATGCATCTACAACCCATCAT
  AAAAGAAAGACAATGAATGATTTTGACTATTTGAAACTGCTAGGTAAAGGCACTTTTGGGAAGGTTATTT
  TGGTTCGAGAGAAGGCAAGTGGAAAATACTACGCTATGAAGATTCTGAAGAAAGAAGTCATTATCGCAAA
  GGATGAAGTGGCACATACTCTGACTGAGAGCAGAGTTCTAAAGAACACCAGACATCCATTTCTAACATCC
  TTGAAATATTCCTTCCAGACAAAAGACCGTTTGTGTTTTGTGATGGAATATGTTAATGGGGGAGAGCTGT
  TTTTCCATTTGTCGAGAGAGCGGGTGTTCTCTGAGGACCGCACACGTTTCTATGGTGCAGAAATTGTCTC
  TGCTTTGGACTATCTACATTCTGGAAAGATTGTGTACCGAGATCTCAAGTTGGAGAATTTGATGCTAGAT
  AAGGATGGCCATATAAAAATTACGGATTTTGGACTTTGCAAAGAAGGGATCACAGATGCAGCTACCATGA
  AGACATTCTGTGGTACACCAGAGTATCTGGCACCAGAGGTATTAGAAGATAATGACTATGGCCGAGCTGT
  GGACTGGTGGGGCCTAGGTGTTGTCATGTATGAAATGATGTGTGGGAGGTTGCCTTTCTACAACCAGGAT
  CATGAGAAACTCTTTGAACTAATACTCATGGAAGACATCAAATTCCCCCGAACACTCTCTTCAGATGCAA
  AGTCATTGCTTTCAGGGCTCTTGATAAAGGATCCAAATAAACGCCTTGGTGGAGGGCCAGATGATCCAAA
  AGAGATAATGAGGCACAGTTTCTTTTCTGGAGTAAACTGGCAGGACGTATATGACAAAAAGCTTGTACCT
  CCTTTTAAGCCTCAAGTAACATCTGAGACAGACACCAGATATTTCGATGAAGAATTTACAGCTCAGACTA
  TTACAATAACACCACCTGAAAAGTGTCCACTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR208375 representing NM_031575
  Red=Cloning site Green=Tags(s)
  
  MSDVTIVKEDWVQKRGEYIKNWRPRYFLLKTDGSFIGYKEKPQDVDLPYPLNNFSVAKCQLMKTERPKPN
  TFIIRCLQWTTVIERTFHVDTPEEREEWTEAIQAVADRLQRQEEERMNCSPTSQIDNIGEEEMDASTTHH
  KRKTMNDFDYLKLLGKGTFGKVILVREKASGKYYAMKILKKEVIIAKDEVAHTLTESRVLKNTRHPFLTS
  LKYSFQTKDRLCFVMEYVNGGELFFHLSRERVFSEDRTRFYGAEIVSALDYLHSGKIVYRDLKLENLMLD
  KDGHIKITDFGLCKEGITDAATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLPFYNQD
  HEKLFELILMEDIKFPRTLSSDAKSLLSGLLIKDPNKRLGGGPDDPKEIMRHSFFSGVNWQDVYDKKLVP
  PFKPQVTSETDTRYFDEEFTAQTITITPPEKCPL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031575
• ORF Size: 1362 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_031575.1, NP_113763.1
• RefSeq Size: 1548
• RefSeq ORF: 1365
• Locus ID: 29414
• MW: 52.8 kDa
• Gene Summary: kinase that associates with both protein kinase C subspecies and beta gamma subunits of G proteins [RGD, Feb 2006]