Cnot8 (NM_001008382) Rat Tagged ORF Clone

CAT#: RR208379

Cnot8 (Myc-DDK-tagged ORF) - Rat CCR4-NOT transcription complex, subunit 8 (Cnot8), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cnot8
• Synonyms: MGC94581
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR208379 representing NM_001008382
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTGCGGCACTTGTAGAAAACAGTCAGGTCATCTGTGAGGTGTGGGCCAGCAATCTTGAGGAGGAGA
  TGCGGAAGATCCGGGAGACTGTCCTCAGCTACAGCTACATCGCCATGGACACAGAGTTTCCAGGTGTTGT
  TGTACGACCGATTGGTGAATTTCGAAGCTCCATAGATTACCAGTATCAGCTTTTGCGGTGCAATGTTGAC
  CTTCTAAAAATCATCCAGCTGGGCCTTACATTCACAAATGAGAAGGGGGAGTACCCTTCTGGAATCAACA
  CGTGGCAGTTCAACTTCAAGTTCAACCTGACAGAGGACATGTACTCCCAGGATTCCATAGACCTGCTAGC
  CAACTCAGGGCTGCAGTTCCAGAAGCACGAGGAGGAAGGGATCGATACGTTGCACTTTGCAGAGCTGCTT
  ATGACGTCGGGAGTGGTCCTCTGTGACAACGTCAAGTGGCTTTCATTTCACAGTGGTTATGATTTTGGCT
  ACATGGTGAAGCTGCTCACGGATTCTCGCCTGCCAGAAGAAGAGCACGAGTTTTTTCACATCCTGAATCT
  TTTCTTCCCATCCATTTATGATGTGAAATACCTGATGAAGAGCTGCAAAAATCTTAAGGGAGGCCTTCAG
  GAGGTGGCTGATCAGTTGGATTTGCAGAGGATTGGAAGGCAGCATCAAGCAGGCTCAGACTCTCTGCTGA
  CAGGGATGGCTTTCTTCAGGATGAAAGAGCTATTCTTTGAGGACAGTATTGATGATGCCAAGTATTGTGG
  GCGCCTCTATGGCCTGGGCACAGGAGTGGCCCAGAAGCAGAATGAGGATGTGGACTCTGCCCAGGAGAAG
  ATGAGCATCTTGGCCATGATCAACAACATGCAGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR208379 representing NM_001008382
  Red=Cloning site Green=Tags(s)
  
  MPAALVENSQVICEVWASNLEEEMRKIRETVLSYSYIAMDTEFPGVVVRPIGEFRSSIDYQYQLLRCNVD
  LLKIIQLGLTFTNEKGEYPSGINTWQFNFKFNLTEDMYSQDSIDLLANSGLQFQKHEEEGIDTLHFAELL
  MTSGVVLCDNVKWLSFHSGYDFGYMVKLLTDSRLPEEEHEFFHILNLFFPSIYDVKYLMKSCKNLKGGLQ
  EVADQLDLQRIGRQHQAGSDSLLTGMAFFRMKELFFEDSIDDAKYCGRLYGLGTGVAQKQNEDVDSAQEK
  MSILAMINNMQQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001008382
• ORF Size: 876 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001008382.1, NP_001008383.1
• RefSeq Size: 2171 bp
• RefSeq ORF: 879 bp
• Locus ID: 363603
• Cytogenetics: 10q22
• MW: 33.5 kDa