Sgpl1 (NM_173116) Rat Tagged ORF Clone

CAT#: RR208430

Sgpl1 (Myc-DDK-tagged ORF) - Rat sphingosine-1-phosphate lyase 1 (Sgpl1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Sgpl1
• Synonyms: S1PL; Spgl1; spl
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR208430 representing NM_173116
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGAGTACCGACCTTCTCAAGTTGAAGGACTTCGAGCCTTATTTGGAGATTTTGGAAGCATATTCCA
  CAAAAGCCAAGAATTACGTGAATGGGTACTGCACCAAATACGAGCCCTGGCAGCTCATTGCGGGGAGTGT
  CCTGTGTACTCTGCTGGTAGTCTGGGTATATGAGCTTATCTTCCAGCCAGAGAGTTTATGGTCTCGGTTT
  AAAAATAAATTATTTAGGCTTATAAGGAAGATGCCATTTATTGGACGTAAGATACAACAGCAGCTTACCA
  AAGCCAAGAAGGATCTTGTCAAGAACATGCCATTCCTGAAGTTGGACAAAGATTATGTGAAAACCCTGCC
  TGCTCAGGGTCTGAGCACAGCTGAGGTTCTGGAGAGACTCAAGGAGTACAGCTCCATGGATGTCTTCTGG
  CAAGAAGGGAAAGCCTCAGGAGCTGTGTACAGTGGGGAGCCGAAGCTCACCGAGCTGCTGGTGCAGGCTT
  ACGGAGAATTCACGTGGAGCAATCCACTGCACCCAGATATCTTCCCCGGACTACGGAAGTTAGAGGCAGA
  AATCGTGAGGATGACTTGCTCCCTCTTCAATGGGGGGCCAGACTCCTGTGGATGTGTGACTTCTGGGGGA
  ACAGAAAGCATCCTAATGGCCTGCAAAGCATATCGGGACTTGGCCTTAGAGAAGGGGATCAAAACTCCAG
  AAATTGTGGCTCCTGAGAGTGCCCACGCCGCATTCGACAAAGCAGCTCATTATTTCGGAATGAAGATTGT
  ACGCGTTGCGCAGAAAAAGAACATGGAGGTGGATGTGCGGGCAATGAAAAGAGCCATCTCCAGGAACACA
  GCCATGCTGGTCTGTTCTGCCCCACAGTTTCCTCATGGTGTGATAGATCCTATCCCCGAAGTGGCCAAGC
  TGGCTGTCAAATATAAAATCCCATTCCATGTGGATGCTTGTCTTGGGGGCTTCCTCATTGTCTTCATGGA
  GAAAGCAGGGTACCCACTGGAGAAACCATTTGATTTCCGGGTGAAAGGTGTGACCAGCATTTCAGCAGAT
  ACTCATAAGTACGGCTATGCTCCCAAAGGTTCATCAGTGGTGATGTACTCTAACGAGAAGTACAGGAAGT
  ACCAGTTCTTTGTTGATGCAGATTGGCAAGGTGGCATCTACGCATCTCCAAGCATAGCTGGCTCACGGCC
  TGGTGGCATCATTGCAGCCTGTTGGGCCGCCTTGATGCACTTTGGTGAGAACGGCTATGTTGAAGCTACC
  AAACAGATCATCAAAACTGCTCGCTTCCTCAAGTCAGAACTGGAAAACATCAAAAACATCTTCATTTTGG
  GGGATCCTCAATTGTCAGTTATTGCTCTGGGCTCCAACGATTTTGACATCTACCGACTATCTAATATGAT
  GTCTGCTAAGGGGTGGAATTTTAACTACTTGCAGTTCCCAAGAAGCATTCATTTCTGCATTACGTTAGTA
  CATACTCGGAAGCGAGTGGCAATCCAGTTCCTAAAGGATATCCGGGAATCGGTCACACAAATCATGAAGA
  ATCCTAAAGCTAAGACCACAGGAATGGGTGCCATCTATGGCATGGCCCAGGCAACCATTGACAGGAAGAT
  GGTTGCAGAAATATCCTCCGTCTTCTTGGACAGCCTTTATAGTACAGACCCTGTGACTCAGGGTAACCAG
  ATGAACGGTTCTCCAAAGCCCCGC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR208430 representing NM_173116
  Red=Cloning site Green=Tags(s)
  
  MPSTDLLKLKDFEPYLEILEAYSTKAKNYVNGYCTKYEPWQLIAGSVLCTLLVVWVYELIFQPESLWSRF
  KNKLFRLIRKMPFIGRKIQQQLTKAKKDLVKNMPFLKLDKDYVKTLPAQGLSTAEVLERLKEYSSMDVFW
  QEGKASGAVYSGEPKLTELLVQAYGEFTWSNPLHPDIFPGLRKLEAEIVRMTCSLFNGGPDSCGCVTSGG
  TESILMACKAYRDLALEKGIKTPEIVAPESAHAAFDKAAHYFGMKIVRVAQKKNMEVDVRAMKRAISRNT
  AMLVCSAPQFPHGVIDPIPEVAKLAVKYKIPFHVDACLGGFLIVFMEKAGYPLEKPFDFRVKGVTSISAD
  THKYGYAPKGSSVVMYSNEKYRKYQFFVDADWQGGIYASPSIAGSRPGGIIAACWAALMHFGENGYVEAT
  KQIIKTARFLKSELENIKNIFILGDPQLSVIALGSNDFDIYRLSNMMSAKGWNFNYLQFPRSIHFCITLV
  HTRKRVAIQFLKDIRESVTQIMKNPKAKTTGMGAIYGMAQATIDRKMVAEISSVFLDSLYSTDPVTQGNQ
  MNGSPKPR
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_173116
• ORF Size: 1704 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_173116.1, NP_775139.1
• RefSeq Size: 2033
• RefSeq ORF: 1707
• Locus ID: 286896
• MW: 63.8 kDa
• Gene Summary: catalyzes the conversion of phosphorylated long chain bases into ethanolamine phosphate and a fatty aldehyde in sphingolipid catabolism [RGD, Feb 2006]