Trim28 (NM_053916) Rat Tagged ORF Clone

CAT#: RR208563

Trim28 (Myc-DDK-tagged ORF) - Rat tripartite motif-containing 28 (Trim28), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Trim28
• Synonyms: Kap1; Krip1; Tif1b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR208563 representing NM_053916
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCTCGGCGGCGGCGGCGACTGCGGCGGCCTCGGCCGCGACGGCCGCGTCAGCGGCCTCTGGTA
  GCCCAGGGTCGGGCGAAGGCTCGGCGGGCGGTGAGAAGCGCCCCGCTGCCTCTTCAGCCGCGGCGGCCTC
  GGCCTCCGCGTCGTCCCCTGCGGGGGGCGGTGGCGAGGCGCAGGAGCTTTTGGAGCACTGCGGCGTGTGT
  CGCGAGCGCTTGCGGCCTGAGCGGGATCCTCGGCTGCTGCCCTGTCTGCATTCGGCCTGTAGTGCCTGCC
  TTGGCCCCGCTACACCCGCCGCAGCTAATAATTCGGGGGATGGCGGCTCGGCGGGCGACGGCGCTATGGT
  GGATTGTCCAGTGTGCAAACAACAGTGCTACTCCAAAGACATCGTGGAGAATTACTTTATGCGTGATAGT
  GGCAGTAAGGCCTCTTCTGATTCCCAGGATGCTAACCAGTGCTGCACTAGCTGTGAAGATAACGCCCCAG
  CCACTAGCTATTGTGTGGAGTGCTCTGAACCACTGTGTGAGACCTGTGTGGAGGCTCACCAGCGGGTGAA
  ATACACCAAGGATCACACTGTGCGCTCCACGGGTCCTGCTAAGACTCGAGATGGAGAACGAACAGTCTAT
  TGTAATGTGCACAAGCATGAACCCCTTGTGCTGTTTTGTGAGAGCTGTGACACGCTCACTTGCCGGGACT
  GCCAACTCAATGCTCACAAGGACCACCAGTACCAATTCTTGGAAGATGCAGTAAGGAACCAACGTAAACT
  CTTGGCTTCACTGGTGAAACGTCTTGGGGACAAACATGCCACACTGCAGAAAAACACCAAGGAGGTTCGA
  AGCTCGATCCGCCAGGTGTCTGATGTGCAGAAGCGAGTGCAGGTTGATGTGAAGATGGCCATTCTGCAGA
  TCATGAAGGAGCTGAATAAACGGGGACGAGTTCTGGTCAATGATGCCCAGAAGGTGACTGAGGGTCAGCA
  GGAACGTCTGGAGCGTCAGCACTGGACCATGACCAAAATCCAGAAGCACCAGGAACACATTTTGCGCTTT
  GCCTCTTGGGCTTTGGAGAGTGATAACAATACAGCCCTCTTGCTCTCTAAGAAGCTGATTTATTTCCAGC
  TGCATCGGGCCCTCAAAATGATTGTGGATCCTGTGGAGCCTCATGGTGAGATGAAGTTTCAGTGGGATCT
  CAATGCCTGGACCAAGAGTGCTGAAGCCTTTGGTAAGATTGTGGCTGAGCGTCCTGGTACAAACTCCACG
  GGTCCTGGGCCCATGGCTCCTCCAAGAGCCCCAGGCCCCCTAAGCAAGCAAGGTTCTGGTAGTAGCCAGC
  CCATGGAAGTACAAGAGGGCTATGGCTTTGGGACAGATGATCCCTACTCAAGTGCAGAGCCACATGTATC
  AGGCATGAAGCGGTCCCGCTCTGGTGAGGGAGAAGTAAGTGGCCTCATGAGGAAGGTGCCACGTGTGAGC
  CTTGAACGCCTGGATCTGGATCTCACCTCTGATAGCCAGCCACCAGTCTTCAAGGTCTTTCCTGGAAGCA
  CCACTGAGGACTACAATCTAATTGTTATTGAGCGTGGTGCTGCTGCAGCAGCCGCTGGCCAGGCTGGGAC
  TGTGCCACCAGGAGCCCCTGGTGCCCCACCCCTGCCTGGCATGGCCATTGTCAAGGAAGAAGAGACAGAA
  GCTGCTATTGGAGCCCCTCCTGCTGCCCCTGAGGGGCCTGAAACCAAGCCTGTGTTGATGGCTCTGACTG
  AAGGCCCTGGGGCTGAGGGACCTCGTCTAGCTTCACCTAGTGGCAGTACCAGCTCAGGCTTGGAGGTGGT
  AGCTCCTGAGGTTACCTCAGCCCCAGTAAGTGGGCCAGGTATCCTGGATGACAGTGCCACTATCTGCCGT
  GTATGCCAGAAGCCAGGTGACCTGGTCATGTGCAACCAGTGCGAGTTTTGCTTCCACCTGGATTGTCACC
  TCCCTTCCCTGCAGGATGTTCCAGGGGAGGAATGGAGTTGCTCACTCTGCCACGTGCTCCCTGATCTAAA
  GGAGGAAGATGGAAGCCTTAGCTTGGATGGAGCAGATAGCACTGGTGTGGTAGCTAAACTCTCACCAGCC
  AACCAGCGGAAATGTGAGCGTGTTCTGCTGGCCCTTTTCTGCCATGAACCGTGCCGCCCCTTGCATCAGC
  TGGCTACCGACTCTACATTCTCCATGGAGCAGCCTGGTGGTACCCTAGACCTGACCTTGATTCGTGCTCG
  CCTCCAAGAGAAGCTGTCACCTCCTTATAGCTCCCCCCAGGAGTTTGCCCAGGATGTGGGCCGCATGTTC
  AAACAGTTCAATAAGCTGACTGAGGACAAGGCAGATGTTCAGTCCATCATCGGCCTCCAGCGCTTCTTTG
  AGACACGAATGAATGATGCCTTTGGTGACACCAAGTTTTCTGCTGTGCTGGTAGAACCACCACCACTGAA
  CCTTCCCAGTGCTGGCCTAAGTTCTCAGGAGCTGTCTGGTCCTGGTGATGGCCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR208563 representing NM_053916
  Red=Cloning site Green=Tags(s)
  
  MAASAAAATAAASAATAASAASGSPGSGEGSAGGEKRPAASSAAAASASASSPAGGGGEAQELLEHCGVC
  RERLRPERDPRLLPCLHSACSACLGPATPAAANNSGDGGSAGDGAMVDCPVCKQQCYSKDIVENYFMRDS
  GSKASSDSQDANQCCTSCEDNAPATSYCVECSEPLCETCVEAHQRVKYTKDHTVRSTGPAKTRDGERTVY
  CNVHKHEPLVLFCESCDTLTCRDCQLNAHKDHQYQFLEDAVRNQRKLLASLVKRLGDKHATLQKNTKEVR
  SSIRQVSDVQKRVQVDVKMAILQIMKELNKRGRVLVNDAQKVTEGQQERLERQHWTMTKIQKHQEHILRF
  ASWALESDNNTALLLSKKLIYFQLHRALKMIVDPVEPHGEMKFQWDLNAWTKSAEAFGKIVAERPGTNST
  GPGPMAPPRAPGPLSKQGSGSSQPMEVQEGYGFGTDDPYSSAEPHVSGMKRSRSGEGEVSGLMRKVPRVS
  LERLDLDLTSDSQPPVFKVFPGSTTEDYNLIVIERGAAAAAAGQAGTVPPGAPGAPPLPGMAIVKEEETE
  AAIGAPPAAPEGPETKPVLMALTEGPGAEGPRLASPSGSTSSGLEVVAPEVTSAPVSGPGILDDSATICR
  VCQKPGDLVMCNQCEFCFHLDCHLPSLQDVPGEEWSCSLCHVLPDLKEEDGSLSLDGADSTGVVAKLSPA
  NQRKCERVLLALFCHEPCRPLHQLATDSTFSMEQPGGTLDLTLIRARLQEKLSPPYSSPQEFAQDVGRMF
  KQFNKLTEDKADVQSIIGLQRFFETRMNDAFGDTKFSAVLVEPPPLNLPSAGLSSQELSGPGDGP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053916
• ORF Size: 2505 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_053916.1, NP_446368.1
• RefSeq Size: 3001
• RefSeq ORF: 2508
• Locus ID: 116698
• MW: 89 kDa
• Gene Summary: mouse homolog is a transcriptional repressor; interacts with the Kruppel-associated box A (KRAB-A) domain of C2H2 zinc finger proteins [RGD, Feb 2006]