Fuca2 (NM_001004218) Rat Tagged ORF Clone

CAT#: RR208621

Fuca2 (Myc-DDK-tagged ORF) - Rat fucosidase, alpha-L- 2, plasma (Fuca2), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Fuca2
• Synonyms: MGC93735
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR208621 representing NM_001004218
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCCTGGGGCTCCTGATGTTTCTGCCGCTACTGCTGCTGGCAACCCGGTACCGGGCCGTGACAGCCC
  TTAGCTATGACCCCACGTGGGAGTCTCTGGACAGACGCCCGCTGCCAGCCTGGTTCGACCAGGCCAAGTT
  CGGCATCTTCATCCACTGGGGAGTGTTTTCCGTGCCCAGCTTTGGTAGTGAATGGTTCTGGTGGTATTGG
  CAAAAGGAAAGGAGACCCAAGTTTGTGGACTTTATGGACAATAATTATCCCCCTGGCTTTAAGTATGAAG
  ATTTTGGAGTGTTATTTACAGCCAAGTATTTTAACGCAAACCAATGGGCAGATCTTCTCCAGGCCTCTGG
  TGCCAAATATGTGGTCTTAACTTCCAAACATCATGAAGGATTCACTCTGTGGGGTTCGGCCCATTCTTGG
  AATTGGAACGCGGTCGATGAGGGGCCGAAGAGGGACATCGTCAAGGAGCTTGAGGTGGCTGTGAGGAACA
  GGACTGACTTGCACTTTGGTCTGTACTATTCTCTTTTTGAATGGTTCCACCCACTCTTTCTGGAGGATCA
  ATCCAGCGCGTTCCAGAAGCAGCGGTTTCCCGTTGCTAAGACGTTGCCTGAGCTCTATGAGTTGGTGACC
  AAGTACCAGCCGGAAGTTCTGTGGTCAGACGGAGATGGGGGAGCGCCAGATCACTACTGGAATAGTACTG
  ACTTCTTGGCCTGGCTCTATAACGAAAGCCCAGTTCGGGACACAGTAGTCACCAATGATCGCTGGGGAGC
  TGGCTCTATCTGCAAACACGGTGGTTACTATACTTGCAGTGACCGTTATAATCCCGGACATCTCTTGCCA
  CACAAGTGGGAGAACTGCATGACAATAGACAAGTTCTCCTGGGGCTACAGACGGGAGGCTGAAATCGGTG
  ACTATCTTACCATTGAAGAATTGGTGAAGCAACTTGTGGAAACGGTCTCATGTGGAGGAAATCTCCTGAT
  GAATATTGGCCCCACACTAGATGGCATCATTCCTGTAATTTTTGAGGAGCGCTTAAGGCAAATGGGAACC
  TGGCTGAAAGTCAACGGGGAAGCTATTTATGAAACACATACTTGGAGGTCTCAGAACGACACCGTCACTC
  CTGACGTGTGGTACACATCTAAACCTGAGAAGAAATTAGTCTATGCTATTTTTCTGAAATGGCCCATCTC
  TGGAAAGCTGTTTCTTGGACAACCCATAGGTAGTCTGGGGGAAACAGAGGTAGAACTACTGGGCCACGGA
  CGGCCACTGACTTGGACATCTTCAAAGCCAAGCGGCATTGTGGTGGAGTTGCCCCGGCTAAGCGTTCATC
  AGATGCCCTGTAAATGGGGATGGACTCTGGCACTAACCAATGTCACT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR208621 representing NM_001004218
  Red=Cloning site Green=Tags(s)
  
  MRLGLLMFLPLLLLATRYRAVTALSYDPTWESLDRRPLPAWFDQAKFGIFIHWGVFSVPSFGSEWFWWYW
  QKERRPKFVDFMDNNYPPGFKYEDFGVLFTAKYFNANQWADLLQASGAKYVVLTSKHHEGFTLWGSAHSW
  NWNAVDEGPKRDIVKELEVAVRNRTDLHFGLYYSLFEWFHPLFLEDQSSAFQKQRFPVAKTLPELYELVT
  KYQPEVLWSDGDGGAPDHYWNSTDFLAWLYNESPVRDTVVTNDRWGAGSICKHGGYYTCSDRYNPGHLLP
  HKWENCMTIDKFSWGYRREAEIGDYLTIEELVKQLVETVSCGGNLLMNIGPTLDGIIPVIFEERLRQMGT
  WLKVNGEAIYETHTWRSQNDTVTPDVWYTSKPEKKLVYAIFLKWPISGKLFLGQPIGSLGETEVELLGHG
  RPLTWTSSKPSGIVVELPRLSVHQMPCKWGWTLALTNVT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001004218
• ORF Size: 1377 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001004218.1, NP_001004218.1
• RefSeq Size: 1630 bp
• RefSeq ORF: 1380 bp
• Locus ID: 292485
• Cytogenetics: 1p13
• MW: 53.3 kDa
• Gene Summary: cytoplasm-specific alpha-L fucosidase; important for carbohydrate and glycosaminoglycan metabolism [RGD, Feb 2006]