Abtb1 (NM_001005902) Rat Tagged ORF Clone

CAT#: RR208652

Abtb1 (Myc-DDK-tagged ORF) - Rat ankyrin repeat and BTB (POZ) domain containing 1 (Abtb1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Abtb1
• Synonyms: MGC94157
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR208652 representing NM_001005902
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACACTAGCGACCTGTTTGCCAGCTGCAGGAAGGGGGACGTGGGCCGTGTGCGGTACCTGCTGGAGC
  AGCGAGACGTGGAGGTGAACGTGCGGGATAAGTGGGACAGCACCCCTTTGTACTATGCCTGCCTCTGTGG
  GCATGAGGAGCTGGTGCGCTACCTGTTGGCTAATGGAGCACGCTGTGAGGCCAACACCTTTGATGGAGAA
  CGGTGTCTCTATGGAGCGCTGAGCGACCCCATTCGCCGTGCACTGAGAGACTACAAACAGGTCACTGCAT
  CCTGCAGGAGGCGGGATTACTATGACGATTTCTTGCAGCGGCTTCTGGAACAGGGCATCCACAGCGATGT
  GGTCTTTGTGGTACACGGAAAGCCATTCCGGGCCCATCGCTGCATCCTCGGTGCTCGCAGTACCTACTTT
  GCCAACATGCTGGACACCAAGTGGAAGGGCAAGAGCGTCGTGGTTCTCAGGCACCCCCTGATCAACCCGG
  TGGCCTTTGGGGCTCTACTGCAGTATCTGTACACAGGCCGCTTGGACATCGGTGTGGAGCATGTAAGTGA
  CTGTGAGCGCTTGGCTAAGCAGTGCCAGCTATGGGACCTGCTCGACGACCTGGAGGCTAAATGCGAGAAG
  GTGTCTGAATTTGTGGCTTCCAAGCCTGGCACATGTGTGAAGGTGCTGACTATTGAGCCCCCTCCAGCAG
  ATCCCCGGTTACGGGCAGATATGGCACTGCTGGCCGACTGTGCCCTGCCACCTGAGCTGCGGGGTGACCT
  TGGAGAGCTGCCCTTCCCATGTCCTGATGGCTTTAGCAGCTGCCCTGATATCTGCTTCCGTGTGGCTGAC
  TCCAGCTTCCTCTGCCACAAGGCTTTCTTCTGTGGGCGCAGTGACTACTTCCGGGCTCTTCTGGATGACC
  ACTTTCGAGAGAGTGAGGAGCCTGTAGCCTCTGGAGACCCCCCAGTTGTCACCCTGCATGACATCTCCCC
  AGACATCTTCACCCATGTCCTGTACTACGTATATAGTGACCATACTGAGCTACCTCCTGAGTTGGCCTAT
  GATGTGCTGAGTGTGGCTGACATGTACCTTCTACCTGGCCTGAAGCGGCTGTGTGGCCGCAGCCTGGCCC
  AGCTACTGGAGGAGGACAGTGTGGTGGGTGTTTGGCGCATAGCCAAGCTGTTCCGGCTGGCCCGGCTGGA
  GGACCAGTGTACCGAGTATATGGCCAAGGTCATCGAGAAGCTGGTGGAGCGAGAGGACTTCGTGGAGGCA
  GTGCGGGAAGAGGCAGCCGCTGTAGCGGCCCGGCAGGAGACAGACTCCATCCCACTTGTTGATGACATCC
  GTTTCCATGTGGCCAGCACAGTCCAGACCTACAGTGCCATCGAAGAGGCACAGCAGCAGCTGCGGGCACT
  CGAGAACCTGCTTGTGTCCATTGGCTTAGACTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR208652 representing NM_001005902
  Red=Cloning site Green=Tags(s)
  
  MDTSDLFASCRKGDVGRVRYLLEQRDVEVNVRDKWDSTPLYYACLCGHEELVRYLLANGARCEANTFDGE
  RCLYGALSDPIRRALRDYKQVTASCRRRDYYDDFLQRLLEQGIHSDVVFVVHGKPFRAHRCILGARSTYF
  ANMLDTKWKGKSVVVLRHPLINPVAFGALLQYLYTGRLDIGVEHVSDCERLAKQCQLWDLLDDLEAKCEK
  VSEFVASKPGTCVKVLTIEPPPADPRLRADMALLADCALPPELRGDLGELPFPCPDGFSSCPDICFRVAD
  SSFLCHKAFFCGRSDYFRALLDDHFRESEEPVASGDPPVVTLHDISPDIFTHVLYYVYSDHTELPPELAY
  DVLSVADMYLLPGLKRLCGRSLAQLLEEDSVVGVWRIAKLFRLARLEDQCTEYMAKVIEKLVEREDFVEA
  VREEAAAVAARQETDSIPLVDDIRFHVASTVQTYSAIEEAQQQLRALENLLVSIGLDC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001005902
• ORF Size: 1434 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001005902.1, NP_001005902.1
• RefSeq Size: 2006 bp
• RefSeq ORF: 1437 bp
• Locus ID: 297432
• Cytogenetics: 4q34
• MW: 54.1 kDa