Hif3a (NM_022528) Rat Tagged ORF Clone

CAT#: RR208820

Hif3a (Myc-DDK-tagged ORF) - Rat hypoxia inducible factor 3, alpha subunit (Hif3a), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hif3a
• Synonyms: Ipas; Mop7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR208820 representing NM_022528
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTGGGACCAAGACAGGTCGAGCACCGAGCTGCGGAAGGAGAAGTCGCGGGATGCGGCCCGCAGCA
  GGCGCAGCCAGGAGACGGAGGTGCTGTACCAACTGGCGCACACCCTGCCCTTTGCGCGCGGCGTCAGCGC
  GCACCTGGACAAGGCCTCCATCATGCGCCTCACAATCAGCTACTTGCGCATGCACCGCCTCTGCGCTGCA
  GGGGAGTGGAACCAGGTGAGAAAAGAGGGAGAACCACTGGACGCCTGCTACCTGAAGGCCCTGGAGGGTT
  TCGTCATGGTGCTCACCGCTGAGGGAGACATGGCTTACCTGTCGGAAAATGTCAGCAAGCACCTGGGCCT
  CAGTCAGCTGGAGCTCATTGGACACAGTATCTTTGATTTTATCCATCCCTGTGACCAAGAGGAACTCCAG
  GATGCCCTGACCCCGAGACCAAGCCTGTCAAAGAAGAAGTCAGAAGCAGCAACAGGACGCCACTTTTCCC
  TGCGAATGAAGAGCACACTCACCAGCAGGGGGCGCGCGCTGAACCTCAAAGCGGCCACCTGGAAGGTGCT
  GCACTGCTCAGGACATATGAGGGCCTACAAGCCCCCTGCACAGACTTCCCCCGCCGGGAGCCCTCGCTCC
  GAGCCTCCCCTGCAATGCCTGGTGCTTATCTGTGAAGCCATCCCCCACCCAGCCAGTCTGGAGCCCCCAC
  TGGGCCGAGGGGCCTTTCTCAGTCGCCACAGCCTGGACATGAAGTTCACATACTGCGACGAGAGGATTGC
  AGAAGTTGCTGGCTACAGCCCCGATGACCTGATTGGCTGTTCTGCCTATGAATACATCCACGCTTTGGAC
  TCTGATGCAGTCAGCAGGAGCATCCACACTTTGCTGAGCAAGGGCCAGGCAGTAACAGGGCAGTATCGCT
  TCCTGGCCCGGACTGGAGGCTATCTGTGGACTCAGACTCAGGCTACAGTGGTGTCAGGGGGGCGGGGCCC
  CCAGTCGGAAAGTATCATCTGCGTCCACTTCCTGATCAGCCGTGTAGAAGAGAACGGAGTGGTGCTGTCC
  CTGGAACAAACGGAGCAACATACTCGCAGACCCCCTCAGCTGGGTACCTCCTCGAAGAAGGGTATCCCAG
  GCAACAGTCTAGACCCTCCCGCTCCACGGATCCTGGCCTTCCTGCACCCTCCAGCCCTGAGTGAGGCCTC
  CCTGGCTGCTGACCCTCGCCGTTTTTGTAGCCCAGACCTGCGCCGCCTCATGGCACCCATCCTGGATGGA
  CCTCCCACAGCCGCTACCCCCAGCACCCCGCAAGCTGCCAGGAGACCCCAAAGTCCTCTTCCGGCTGATC
  TCCCAGATCAGTTGGCTGTGGGCTTGGAGAATGCACACAGACTCTCCACTGCCCGGAAAAACAAGACCAT
  GGAGACAGATCTAGATATAGCTCAGGACCCTGACACTCCGGACTTGGAGATGTTGGCTCCGTACATCTCC
  ATGGATGATGACTTCCAGCTCAACTCCAGCGAGCAATTGCCCAAAGTCCACCGCAGACCTCCCAGGACGG
  CCCGCAGGCCTCGTGCTCGGAGCTTCCATGGCCTGTCGCCTCCCATCCCTGAGGCCACCCTGCTGCCCCG
  CTGGGGGAGTGATCCACGACTGAACTGTTCCAGCCCCTCCAAGGGGGATCCCCCCACAGCCCCCCTGACG
  CCTAGAACTCGGAAGAGGGCCTTGGCCCAGAGCTCAGAGGACAAAGGGTTGGAGCTGCTGGAAACGAAGC
  CCCCCAAGCGGTCCCCAAGACTAGAACCTGGAAGTGTCCTGCTGCCTCCGCTCAGCCTGAGTTTCCTTCT
  GCAAGGTCGACAACTTCCAGGGAACCAGCCGGATCCCAGAGCCCCACTCGTGGATTCGCATGAGCCCTTG
  GGCCTAGCTCCCTCGCTGCTCTCTCTCTACCAGCATGAGGAAACTATCCAGTCCAGGAACCACTTCCTCC
  CAGCAGCAGGCTTGGCCCAGACACAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR208820 representing NM_022528
  Red=Cloning site Green=Tags(s)
  
  MDWDQDRSSTELRKEKSRDAARSRRSQETEVLYQLAHTLPFARGVSAHLDKASIMRLTISYLRMHRLCAA
  GEWNQVRKEGEPLDACYLKALEGFVMVLTAEGDMAYLSENVSKHLGLSQLELIGHSIFDFIHPCDQEELQ
  DALTPRPSLSKKKSEAATGRHFSLRMKSTLTSRGRALNLKAATWKVLHCSGHMRAYKPPAQTSPAGSPRS
  EPPLQCLVLICEAIPHPASLEPPLGRGAFLSRHSLDMKFTYCDERIAEVAGYSPDDLIGCSAYEYIHALD
  SDAVSRSIHTLLSKGQAVTGQYRFLARTGGYLWTQTQATVVSGGRGPQSESIICVHFLISRVEENGVVLS
  LEQTEQHTRRPPQLGTSSKKGIPGNSLDPPAPRILAFLHPPALSEASLAADPRRFCSPDLRRLMAPILDG
  PPTAATPSTPQAARRPQSPLPADLPDQLAVGLENAHRLSTARKNKTMETDLDIAQDPDTPDLEMLAPYIS
  MDDDFQLNSSEQLPKVHRRPPRTARRPRARSFHGLSPPIPEATLLPRWGSDPRLNCSSPSKGDPPTAPLT
  PRTRKRALAQSSEDKGLELLETKPPKRSPRLEPGSVLLPPLSLSFLLQGRQLPGNQPDPRAPLVDSHEPL
  GLAPSLLSLYQHEETIQSRNHFLPAAGLAQTH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022528
• ORF Size: 1986 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_022528.1, NM_022528.2, NP_071973.1
• RefSeq Size: 2076
• RefSeq ORF: 1989
• Locus ID: 64345
• MW: 72.9 kDa
• Gene Summary: binds HIF-responsive elements and activates transcription in vitro [RGD, Feb 2006]