Ddx27 (NM_001135801) Rat Tagged ORF Clone

CAT#: RR208859

Ddx27 (Myc-DDK-tagged ORF) - Rat DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 (Ddx27), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ddx27
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR208859 representing NM_001135801
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGCGGAGCTCGGTTTCATCGGGACCATCGGCGAAAATGACGAGGTGCCGGTTGAACCCGAGTCGG
  ATTCCGGAGACGAGGAGGAGGAGGGACCCATTGTGCTGGGCCTCAAGCAGAAAGCCCTGCAGAAGAACCG
  CAGTGCTGACTTCAACCCTGACTTTGTTTTCACTGAGAAGCAGGGCACGTATGATGGTAGCTGGGCCCTG
  GCTGACGTCATGAGCCAGCTCAAGAAGAAGAGGGCAGCCACTACCCTGGATGAGAAGATTGAGAAAGTAC
  GGAAGAAAAGGAAAGCAGAGGATAAGGAAGCCAAGTCTGGGAAGGCAGAGGAGAAAGAAGGCCAGGAGGG
  CTCTGACCTGAAGGGGCAGGAGAATCTAGGAGAGAATGAGGCAGGCTCCAAGGACGAAGAGTCTGAGACT
  GACTACTCCTCTGAGGATGAGGAGATCCTCACCAAAGCAGACACACTCAAAGTGAAGGAGAAGAAGAAGA
  AGAAGAAGGGCCAGGAATCAGGAGGATTCTTTGAAGATGCATCTGAATATGACAAAAGCCTGACCTTCCA
  GGACATGAACCTCTCCCGGCCCCTCCTGAAGGCCATTACAGCCATGGGCTTCAAGCAGCCCACTCCAATT
  CAGAAGGCATGCATTCCTGTGGGTCTGCTGGGGAAAGACATCTGCGCCTGCGCAGCTACTGGGACAGGCA
  AAACGGCAGCCTTTGCACTGCCAGTCTTAGAGCGTCTGATCTACAAGCCCCGTCAGGCTGCGGTCACTCG
  AGTGCTGGTGCTGGTCCCCACCAGAGAGCTGGGCATCCAAGTGCACTCTGTCACCAAGCAGCTGGCCCAG
  TTCTGCAGCATCACTACCTGCCTGGCTGTGGGGGGCCTGGATGTGAAGTCTCAGGAAGCCGCTCTTCGGG
  CAGCACCAGACATCCTCATTGCCACTCCTGGCCGGCTCATCGACCACCTCCACAACTGCCCTTCCTTCCA
  CCTGAGCAGCATTGAAGTGCTCATCTTGGACGAGGCTGACAGGATGCTGGATGAGTATTTTGAGGAGCAG
  ATGAAGGAGATTATCCGAATGTGTTCCCACCACCGCCAGACCATGCTCTTCTCGGCCACCATGACAGATG
  AGGTGAAAGATCTGGCTTCGGTCTCCTTAAAGAATCCTGTGCGGATATTTGTGAACAGCAACACAGATGT
  AGCTCCCTTCCTACGGCAAGAGTTCATCCGGATCCGGCCTAACAGGGAAGGGGACCGGGAAGCCATTGTG
  GCAGCTCTACTGATGAGAACCTTTACTGACCACGTGATGCTGTTCACCCAGACCAAGAAGCAGGCCCATC
  GCATGCACATCCTCCTAGGGCTGCTGGGGCTGCAGGTGGGCGAGCTCCATGGTAACTTGTCACAGACGCA
  GCGGCTGGAAGCTCTCCGGCGCTTTAAGGATGAACAGATTGACATCCTGGTAGCCACAGATGTGGCAGCC
  CGTGGGCTTGACATTGAAGGAGTGAAAACAGTGATCAACTTCACCATGCCCAACACTGTCAAACACTATG
  TGCACCGGGTGGGGCGAACTGCACGTGCTGGCAGGGCTGGGCGCTCTGTCTCTCTGGTGGGAGAAGAGGA
  GCGGAAGATGCTGAAGGAGATCGTAAAGGCTGCCAAGGCTCCAGTGAAGGCTCGGATCCTTCCCCAGGAT
  GTTATCCTCAAATTCCGGGACAAGATTGAGAAAATGGAGAAAGATGTGTATGCAGTGCTGCAGCTGGAGG
  CGGAGGAGAGAGAGGTGCAGCAGTCAGAAGCCCAGATCGACACAGCACAGCGACTCTTATCCAAAGGGAA
  ACAGACAGCAGACCAGGAACCTGAGAGGAGCTGGTTTCAGACCAAAGAAGAGAGGAAAAAGGAGAAGATT
  GCCAAGGCTCTGCAGGAGTTTGACTTGGCCTTAAGAGGAAAGAAGAAAAGGAAGAAGTTTATGAAGGACG
  TCAAGAAAAAGGGGGAGATGACGGCGGAGGAGAGGTCCCAGTTTGAAATCCTCAAGGCACAGATGTTTGC
  AGAGCGGCTGGCCAAGAGGAACCGCAGAACCAAGCGAGCACGGGCAATGCCTGAGGAAGAGCCTGCAGCA
  GGCCCTGCCAAGAAGCAGAAGCAGCACAAGAAGTCTGTGTTTGATGAAGAACTCACCAACACAAGCAAGA
  AGGCCCTGAAACAGTACCGGGCTGGCCCTTCCTTTGAAGAGAGGAAACAGTTAGGTCTGCCCCGCCAGAG
  GAGAGGGAACTTCAAGTCTAAGTCCAGGTACAAGAGAAAGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR208859 representing NM_001135801
  Red=Cloning site Green=Tags(s)
  
  MLAELGFIGTIGENDEVPVEPESDSGDEEEEGPIVLGLKQKALQKNRSADFNPDFVFTEKQGTYDGSWAL
  ADVMSQLKKKRAATTLDEKIEKVRKKRKAEDKEAKSGKAEEKEGQEGSDLKGQENLGENEAGSKDEESET
  DYSSEDEEILTKADTLKVKEKKKKKKGQESGGFFEDASEYDKSLTFQDMNLSRPLLKAITAMGFKQPTPI
  QKACIPVGLLGKDICACAATGTGKTAAFALPVLERLIYKPRQAAVTRVLVLVPTRELGIQVHSVTKQLAQ
  FCSITTCLAVGGLDVKSQEAALRAAPDILIATPGRLIDHLHNCPSFHLSSIEVLILDEADRMLDEYFEEQ
  MKEIIRMCSHHRQTMLFSATMTDEVKDLASVSLKNPVRIFVNSNTDVAPFLRQEFIRIRPNREGDREAIV
  AALLMRTFTDHVMLFTQTKKQAHRMHILLGLLGLQVGELHGNLSQTQRLEALRRFKDEQIDILVATDVAA
  RGLDIEGVKTVINFTMPNTVKHYVHRVGRTARAGRAGRSVSLVGEEERKMLKEIVKAAKAPVKARILPQD
  VILKFRDKIEKMEKDVYAVLQLEAEEREVQQSEAQIDTAQRLLSKGKQTADQEPERSWFQTKEERKKEKI
  AKALQEFDLALRGKKKRKKFMKDVKKKGEMTAEERSQFEILKAQMFAERLAKRNRRTKRARAMPEEEPAA
  GPAKKQKQHKKSVFDEELTNTSKKALKQYRAGPSFEERKQLGLPRQRRGNFKSKSRYKRKK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001135801
• ORF Size: 2283 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001135801.1, NP_001129273.1
• RefSeq Size: 2608
• RefSeq ORF: 2286
• Locus ID: 362274
• MW: 86 kDa