Trim32 (NM_001012103) Rat Tagged ORF Clone

CAT#: RR208860

Trim32 (Myc-DDK-tagged ORF) - Rat tripartite motif protein 32 (Trim32), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Trim32
• Synonyms: MGC109467
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR208860 representing NM_001012103
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCGGCTGCAGCAGCGTCTCACCTGAACCTGGATGCCCTCCGGGAAGTGCTAGAATGTCCCATCT
  GCATGGAATCCTTCACTGAAGAGCAGCTGCGGCCCAAGCTGCTGCACTGTGGCCATACCATCTGCCGCCA
  GTGTCTGGAGAAGCTCCTGGCCAGTAGCATCAATGGTGTCCGCTGTCCCTTTTGCAGCAAGATTACTCGC
  ATCACCAGCCTGACCCAGCTGACCGACAACCTGACAGTGCTGAAGATCATTGACACAGCCGGCCTCAGTG
  AGGCCGTCGGGCTGCTCATGTGCCGAGCCTGTGGCAGGCGGCTGCCTCGGCAGTTCTGCCGAAGCTGTGG
  TTTGGTGTTGTGTGAACCCTGCCGAGAGGCAGATCACCAACCTCCTGGCCACTGCACACTCCCTGTCAAG
  GAGGCAGCTGAGGAACGTCGGAGGGACTTTGGGGAGAAGTTGACTCGTCTAAGGGAACTTACTGGAGAGC
  TGCAAAGGAGGAAGGCAGCTTTAGAGGGTGTCTCTAGAGATCTTCAGGCAAGGTATAAGGCTGTTCTTCA
  AGAATATGGCCACGAGGAACGCAGGGTCCAGGAAGAGCTAGCCCGCTCTCGGAAGTTCTTCACAGGCTCT
  TTGGCTGAGGTTGAGAAGTCCAACAGCCAAGTAGTAGAGGAACAGAGCTACCTACTCAACATTGCGGAGG
  TGCAGGCCGTGTCTCGCTGTGACTACTTTCTAGCCAAGATCAAGCAAGCTGATGTAGCACTCCTGGAGGA
  GACAGCAGATGAGGAGGAGCCAGAGCTCACTGCAAGCCTACCCCGGGAGCTTACCCTGCAAGATGTGGAG
  CTTCTTAAAGTAGGACATGTTGGTCCTCTGCAAATTGGCCAGGCTGTTAAGAAGCCCCGGACAGTTAACA
  TGGAAGATTCCTGGGCAGTGGAGGAGGGAGCAGCATCTTCTGCCTCAGCCTCTGTAACCTTTAGAGAGAT
  GGACATGAGCCCTGAAGAAGTAGTTCCCAGCCCTAGGGCTTCACCTGCGAAACAGCGGAGTTCTGAGGCA
  GCCTCCAGTATCCAACAGTGTCTCTTTCTCAAGAAGATGGGAGCGAAAGGCAGCACTCCAGGCATGTTCA
  ACCTTCCAGTCAGTCTCTATGTGACCAGTCAGAGTGAGGTGCTGGTTGCAGACCGGGGCAACTATCGCAT
  ACAAGTGTTCAACCGCAAAGGCTTTTTGAAGGAGATCCGCCGCAGCCCCAGTGGCATTGACAGCTTTGTG
  CTAAGCTTCCTTGGAGCCGACTTGCCCAATCTCACTCCTCTTTCAGTGGCCATGAACTGCCATGGGCTGA
  TTGGTGTCACTGACAGCTATGACAACTCCCTTAAAGTCTATACCTTGGATGGCCACTGTGTGGCCTGTCA
  CAGGAGCCAGCTGAGCAAACCATGGGGCATCACAGCCCTACCGTCTGGCCAGTTTGTTGTGACTGATGTG
  GAAGGCGGGAAGCTCTGGTGTTTCACTGTGGACCGAGGAGCTGGAGTAGTCAAATACAGCTGCCTCTGCA
  GTGCTGTGAGGCCCAAGTTTGTCACCTGTGATGCTGAAGGCACAGTCTATTTCACCCAAGGCTTGGGTCT
  CAATGTGGAAAACCGACAGAATGAACACCACCTGGAGGGTGGCTTCTCCATCGGCTCTGTGGGCCCCGAT
  GGGCAGCTGGGCCGACAGATCAGCCACTTCTTCTCTGAGAATGAAGATTTCCGCTGCATCGCTGGCATGT
  GTGTGGATGCTCGGGGTGACCTCATTGTGGCTGATAGCAGCCGCAAGGAAATCCTCCATTTTCCCAAGGG
  CGGTGGCTACAGCGTCCTTATTCGAGAGGGCCTTACCTGTCCAGTGGGCATCGCCCTCACACCCAAGGGG
  CAGCTGCTGGTCTTGGACTGTTGGGATCACTGTGTCAAGATCTACAGCTACCATCTGAGAAGATACTCTA
  CCCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR208860 representing NM_001012103
  Red=Cloning site Green=Tags(s)
  
  MAAAAAASHLNLDALREVLECPICMESFTEEQLRPKLLHCGHTICRQCLEKLLASSINGVRCPFCSKITR
  ITSLTQLTDNLTVLKIIDTAGLSEAVGLLMCRACGRRLPRQFCRSCGLVLCEPCREADHQPPGHCTLPVK
  EAAEERRRDFGEKLTRLRELTGELQRRKAALEGVSRDLQARYKAVLQEYGHEERRVQEELARSRKFFTGS
  LAEVEKSNSQVVEEQSYLLNIAEVQAVSRCDYFLAKIKQADVALLEETADEEEPELTASLPRELTLQDVE
  LLKVGHVGPLQIGQAVKKPRTVNMEDSWAVEEGAASSASASVTFREMDMSPEEVVPSPRASPAKQRSSEA
  ASSIQQCLFLKKMGAKGSTPGMFNLPVSLYVTSQSEVLVADRGNYRIQVFNRKGFLKEIRRSPSGIDSFV
  LSFLGADLPNLTPLSVAMNCHGLIGVTDSYDNSLKVYTLDGHCVACHRSQLSKPWGITALPSGQFVVTDV
  EGGKLWCFTVDRGAGVVKYSCLCSAVRPKFVTCDAEGTVYFTQGLGLNVENRQNEHHLEGGFSIGSVGPD
  GQLGRQISHFFSENEDFRCIAGMCVDARGDLIVADSSRKEILHFPKGGGYSVLIREGLTCPVGIALTPKG
  QLLVLDCWDHCVKIYSYHLRRYSTP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001012103
• ORF Size: 1965 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012103.1, NP_001012103.1
• RefSeq Size: 3190 bp
• RefSeq ORF: 1968 bp
• Locus ID: 313264
• Cytogenetics: 5q24
• MW: 72.2 kDa