Hadha (NM_130826) Rat Tagged ORF Clone

CAT#: RR208881

Hadha (Myc-DDK-tagged ORF) - Rat hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunit (Hadha), nuclear gen, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hadha
• Synonyms: RGD1560655
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR208881 representing NM_130826
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGCGTCCCGGGCAATTGGCAGTCTCAGTCGCTTCTCTGCCTTCAGGATCCTGCGCTCCAGAGGCT
  GCATTTGCCACAGCTTTACAACTTCTTCTGCTTTGCTGTCTAGAACCCATATTAATTATGGAGTCAAAGG
  GGATGTGGCAGTTATTCGGATTAACTCGCCCAATTCAAAGGTAAATACATTGAATAAAGAAGTACAATCA
  GAGTTCGTAGAAGTAATGAACGAAATCTGGGCCAACGACCAAATCAGGAGCGCCGTCCTTATTTCGTCAA
  AGCCTGGCTGCTTTGTTGCAGGTGCTGACATCAACATGCTGGCCTCTTGTACAACGCCCCAAGAAGCAGC
  ACGAATATCACAAGAAGGACAGAAAATGTTTGAGAAACTTGAAAAGTCACCAAAGCCTGTTGTTGCCGCC
  ATCAGTGGATCCTGCTTGGGAGGCGGACTTGAGCTTGCCATAGCATGTCAATACAGAATAGCAACAAAAG
  ACAGAAAAACAGTATTAGGTGTCCCTGAAGTGTTGCTGGGAATCTTACCAGGAGCCGGAGGTACCCAGAG
  GCTGCCCAAAATGGTGGGTGTGCCTGCTGCTTTTGACATGATGCTGACTGGTAGGAACATTCGTGCAGAC
  AGAGCAAAGAAAATGGGACTGGTTGACCAGTTGGTGGACCCGCTAGGACCAGGAATAAAATCTCCAGAGG
  AAAGGACAATTGAATACCTAGAAGAAGTTGCAGTTAATTTTGCCAAAGGCCTGGCTGACAGGAAGGTCTC
  TGCAAAGCAGAGCAAAGGCCTGATGGAAAAGCTGACATCGTATGCCATGACTATCCCATTTGTCAGACAA
  CAGGTTTACAAAACAGTGGAAGAAAAAGTGAAGAAGCAGACCAAAGGCCTTTACCCTGCACCTTTGAAGA
  TAATTGACGCTGTGAAGACTGGACTTGAGCAAGGAAATGATGCTGGCTATCTTGCCGAATCAGAGAAATT
  TGGAGAGCTTGCATTGACCAAAGAATCAAAAGCCCTGATGGGGCTTTATAATGGCCAGGTCCTGTGCAAG
  AAAAATAAATTTGGAGCGCCACAGAAGACTGTTCAGCAGCTAGCCATCCTTGGCGCAGGGCTGATGGGGG
  CTGGCATTGCCCAGGTCTCTGTGGACAAGGGACTGAAAACTCTTCTTAAAGACACTACAGTGACAGGGCT
  GGGCCGGGGACAGCAACAAGTGTTCAAAGGACTGAATGACAAGGTAAAGAAGAAGGCACTCACATCCTTC
  GAAAGGGACTCCATCTTCAGCAACCTGATCGGGCAGCTCGACTACAAGGGCTTCGAGAAGGCTGACATGG
  TGATTGAGGCTGTCTTCGAGGACCTCGCTGTTAAGCACAAAGTGTTAAAGGAAGTGGAAAGCGTGACTCC
  AGAACACTGTATCTTCGCCAGCAACACATCTGCTCTCCCAATCAATCAAATTGCTGCTGTGAGCCAAAGG
  CCTGAGAAGGTGATCGGCATGCACTACTTCTCTCCTGTGGACAAGATGCAGCTTCTAGAGATCATCACAA
  CTGACAAAACCTCCAAGGACACCACAGCGTCTGCCGTGGCCGTGGGTCTCAAGCAGGGGAAGGTCATCAT
  TGTGGTCAAGGACGGACCTGGCTTCTACACCACCAGGTGTCTTGCTCCCATGATGTCAGAAGTCATAAGA
  ATCCTCCAGGAAGGAGTTGACCCTAAGAAGCTGGACGCCTTGACCACAGGCTTCGGCTTCCCTGTGGGTG
  CTGCCACCCTGGCAGATGAAGTAGGGATAGATGTAGCACAGCACGTAGCAGAAGATCTAGGCAAAGCCTT
  CGGGGAGCGGTTTGGAGGTGGCAGCGTAGAACTGCTGAAACTGATGGTCTCCAAGGGCTTCTTGGGTCGC
  AAGTCTGGGAAGGGCTTCTACATCTATCAGTCGGGCTCAAAGAATAAGAATTTGAATTCTGAAATAGATA
  ATATCTTGGTAAACCTGAGGCTGCCTGCCAAGCCCGAGGTCTCCTCTGATGAAGACATCCAGTACCGTGT
  GATAACAAGGTTTGTGAATGAGGCAGTCCTGTGCCTACAGGAAGGGATCCTAGCCACGCCTGAAGAGGGA
  GACATCGGAGCAGTCTTTGGGCTTGGCTTTCCCCCTTGTCTCGGAGGGCCCTTCCGCTTTGTGGATCTGT
  ATGGTGCTCAGAAGGTAGTGGACCGGCTCCGGAAGTATGAGTCTGCCTATGGGACACAGTTTACCCCGTG
  TCAGCTACTCCGCGACCTTGCTAACAACTCTAGCAAGAAGTTCTACCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR208881 representing NM_130826
  Red=Cloning site Green=Tags(s)
  
  MVASRAIGSLSRFSAFRILRSRGCICHSFTTSSALLSRTHINYGVKGDVAVIRINSPNSKVNTLNKEVQS
  EFVEVMNEIWANDQIRSAVLISSKPGCFVAGADINMLASCTTPQEAARISQEGQKMFEKLEKSPKPVVAA
  ISGSCLGGGLELAIACQYRIATKDRKTVLGVPEVLLGILPGAGGTQRLPKMVGVPAAFDMMLTGRNIRAD
  RAKKMGLVDQLVDPLGPGIKSPEERTIEYLEEVAVNFAKGLADRKVSAKQSKGLMEKLTSYAMTIPFVRQ
  QVYKTVEEKVKKQTKGLYPAPLKIIDAVKTGLEQGNDAGYLAESEKFGELALTKESKALMGLYNGQVLCK
  KNKFGAPQKTVQQLAILGAGLMGAGIAQVSVDKGLKTLLKDTTVTGLGRGQQQVFKGLNDKVKKKALTSF
  ERDSIFSNLIGQLDYKGFEKADMVIEAVFEDLAVKHKVLKEVESVTPEHCIFASNTSALPINQIAAVSQR
  PEKVIGMHYFSPVDKMQLLEIITTDKTSKDTTASAVAVGLKQGKVIIVVKDGPGFYTTRCLAPMMSEVIR
  ILQEGVDPKKLDALTTGFGFPVGAATLADEVGIDVAQHVAEDLGKAFGERFGGGSVELLKLMVSKGFLGR
  KSGKGFYIYQSGSKNKNLNSEIDNILVNLRLPAKPEVSSDEDIQYRVITRFVNEAVLCLQEGILATPEEG
  DIGAVFGLGFPPCLGGPFRFVDLYGAQKVVDRLRKYESAYGTQFTPCQLLRDLANNSSKKFYQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_130826
• ORF Size: 2289 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_130826.1, NM_130826.2, NP_570839.2
• RefSeq Size: 2560
• RefSeq ORF: 2292
• Locus ID: 170670
• MW: 82.7 kDa
• Gene Summary: alpha subunit of the liver mitochondrial fatty acid oxidation multienzyme complex [RGD, Feb 2006]