Uchl5 (NM_001012149) Rat Tagged ORF Clone

CAT#: RR208897

Uchl5 (Myc-DDK-tagged ORF) - Rat ubiquitin carboxyl-terminal hydrolase L5 (Uchl5), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Uchl5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR208897 representing NM_001012149
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGAGCAATGCCGGGGAGTGGTGTCTCATGGAAAGCGACCCCGGGGTCTTCACCGAGCTCATTAAAG
  GATTCGGTTGCCGAGGAGCTCAAGTAGAAGAAATATGGAGCTTAGAGCCTGAGAATTTCGAAAAGCTAAA
  GCCGGTTCATGGGTTAATTTTTCTTTTCAAGTGGCAGCCAGGAGAAGAACCAGCTGGCTCTGTGGTTCAG
  GACTCCAGACTTGAAACAATATTTTTTGCTAAGCAGGTAATTAATAATGCTTGTGCCACGCAGGCTATCG
  TAAGTGTACTACTGAACTGTACCCATCAAGATGTCCATTTAGGAGAGACATTATCAGAATTTAAGGAATT
  TTCCCAAAGTTTTGATGCAGCTATGAAGGGGTTGGCATTGAGTAACTCAGATGTGATTCGACAAGTGCAC
  AACAGTTTTGCCAGACAGCAGATGTTTGAATTTGATACAAAGACATCAGCCAAAGAAGAAGATGCTTTTC
  ACTTTGTCAGTTATGTTCCAGTTAATGGAAGACTGTATGAATTAGATGGATTAAGAGAAGGCCCAATTGA
  TTTAGGTGCATGCAATCAGGATGACTGGATTACTGCAGTGAGGCCAGTAATAGAGAAGAGGATTCAAAAG
  TATAGTGAAGGAGAAATTCGATTTAATTTAATGGCCATTGTATCTGACAGAAAAATGATATATGAACAGA
  AGATAGCAGAGTTACAGAGACAGCTTGCAGAGGAGGAACCCATGGATACAGATCAGGGTAGTACTGTGTT
  AAGTGCTATTCAGTCAGAAGTTGCCAGAAACCAGATGCTTATTGAAGAAGAGGTACAAAAGTTAAAAAGA
  TATAAGATTGAAAATATCAGAAGGAAGCATAACTACCTGCCTTTCATTATGGAATTGTTAAAGACCTTAG
  CAGAACACCAGCAGTTAATTCCACTTGTGGAAAAGATATTCTCATGTAGGGGAAGGAATCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR208897 representing NM_001012149
  Red=Cloning site Green=Tags(s)
  
  MSSNAGEWCLMESDPGVFTELIKGFGCRGAQVEEIWSLEPENFEKLKPVHGLIFLFKWQPGEEPAGSVVQ
  DSRLETIFFAKQVINNACATQAIVSVLLNCTHQDVHLGETLSEFKEFSQSFDAAMKGLALSNSDVIRQVH
  NSFARQQMFEFDTKTSAKEEDAFHFVSYVPVNGRLYELDGLREGPIDLGACNQDDWITAVRPVIEKRIQK
  YSEGEIRFNLMAIVSDRKMIYEQKIAELQRQLAEEEPMDTDQGSTVLSAIQSEVARNQMLIEEEVQKLKR
  YKIENIRRKHNYLPFIMELLKTLAEHQQLIPLVEKIFSCRGRNL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001012149
• ORF Size: 972 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012149.1, NP_001012149.1
• RefSeq Size: 1824 bp
• RefSeq ORF: 975 bp
• Locus ID: 360853
• Cytogenetics: 13q21
• MW: 37.1 kDa