Psmd2 (NM_001031639) Rat Tagged ORF Clone

CAT#: RR209024

Psmd2 (Myc-DDK-tagged ORF) - Rat proteasome (prosome, macropain) 26S subunit, non-ATPase, 2 (Psmd2), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Psmd2
• Synonyms: MGC116280
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209024 representing NM_001031639
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGAGGGTGGACGAGACAAGACACCCGTGCAGTCCCAACAACCCTCAGCGACAGCCCCCAGCGGCG
  CGGACGAGAAGTCGAGCGGCAAGGAGCGACGGGATGCCGGGGAAAAAGATAAAGAGCAGGAGCTGTCTGA
  GGAGGACAAACAGCTTCAAGATGAACTGGAGATGCTCGTGGAGCGACTCGGGGAAAAGGATACATCCCTG
  TACCGACCAGCTCTGGAGGAACTGAGAAGACAAATCCGTTCTTCTACAACTTCCATGACCTCAGTACCGA
  AACCTCTCAAATTTCTGCGTCCACACTATGGCAAACTGAAGGAAATCTATGAGAACATGGCCCCTGGGGA
  GAATAAGTGCTTTGCTGCTGACATCATCTCTGTTTTGGCCATGACCATGAGTGGTGAGCGGGAGTGCCTC
  AAGTATCGGCTAGTGGGCTCCCAGGAAGAATTGGCATCGTGGGGTCATGAGTATGTCAGACATCTGGCAG
  GAGAAGTGGCTAAGGAGTGGCAGGAACTGGATGATGCAGAGAAAGCACAGCGGGAACCATTGCTTACCTT
  GGTAAAGGAAATCGTCCCCTACAACATGGCTCACAATGCTGAGCACGAGGCTTGTGACCTGCTCATGGAG
  ATCGAGCAGGTGGATATGCTGGAGAAGGACATCGATGAGAATGCATACTCCAAGGTCTGCCTCTATCTCA
  CCAGTTGTGTGAATTATGTACCAGAACCTGAGAACTCTGCCCTCCTGCGCTGTGCCCTGGGCGTGTTCCG
  AAAGTTTAGCCGTTTCCCTGAAGCTCTGAGATTGGCACTGATGCTCAATGACATGGAACTGGTAGAAGAC
  ATTTTCACCTCCTGCAAGGATGTGGTGGTCCAGAAGCAGATGGCCTTCATGCTGGGTCGGCATGGAGTGT
  TCCTGGAGCTGAGTGAAGATGTAGAGGAATACGAAGACCTCACAGAAATCATGTCCAATGTCCAGCTCAA
  CAGCAACTTCTTGGCCTTAGCTCGGGAGCTGGACATCATGGAGCCCAAGGTGCCTGATGACATCTACAAA
  ACCCACCTAGAGAATAACAGGTTTGGTGGCAGTGGCTCTCAGGTAGATTCTGCTCGAATGAACCTAGCGT
  CCTCTTTTGTGAATGGCTTTGTGAACGCAGCCTTTGGTCAGGATAAACTCCTGACCGACGATGGCAACAA
  ATGGCTGTACAAGAACAAGGACCACGGGATGTTAAGTGCTGCTGCGTCCCTTGGCATGATTCTGCTGTGG
  GATGTGGATGGTGGCCTCACCCAGATTGACAAGTATCTGTACTCCTCTGAGGACTACATCAAGTCAGGAG
  CTCTCCTTGCCTGTGGTATCGTGAATTCTGGGGTTCGGAATGAGTGCGATCCTGCCCTGGCACTGCTTTC
  CGACTACGTTCTCCATAACAGCAATACCATGAGACTTGGCTCCATCTTCGGACTAGGTTTGGCCTATGCT
  GGCTCTAATCGGGAAGATGTTCTAACACTGCTGCTACCTGTAATGGGAGATTCCAAGTCCAGCATGGAGG
  TCGCAGGTGTGACGGCTCTGGCTTGTGGGATGATAGCAGTGGGGTCTTGCAATGGAGATGTCACTTCGAC
  CATTCTTCAGACCATCATGGAGAAATCTGAGACTGAGCTCAAGGACACCTACGCTCGTTGGCTTCCTCTT
  GGCCTGGGCCTCAATCACTTGGGGAAGGGCGAAGCCATCGAGGCGATCCTGGCTGCCCTGGAGGTTGTGT
  CAGAACCGTTCCGCAGCTTTGCCAACACTCTGGTGGATGTGTGTGCCTATGCAGGTTCCGGGAACGTGCT
  GAAGGTTCAGCAGCTCCTCCACATCTGCAGTGAGCACTTCGACTCTAAGGAGAAGGAAGAAGACAAGGAC
  AAGAAGGAAAAGAAGGACAAGGACAAGAAGGAGGCCCCTGCCGACATGGGAGCACATCAGGGAGTAGCTG
  TTCTGGGAATCGCACTTATTGCTATGGGGGAAGAGATTGGTGCAGAGATGGCATTGCGAACTTTTGGTCA
  TTTGCTGAGATATGGGGAACCTACCCTCCGTCGGGCTGTACCGTTAGCACTGGCACTAATCTCTGTTTCA
  AATCCACGACTCAACATCCTGGATACCCTAAGCAAATTCTCTCATGATGCTGATCCAGAAGTTTCTTATA
  ACTCCATCTTTGCTATGGGCATGGTGGGCAGTGGTACCAACAATGCCCGTCTGGCTGCAATGCTGCGCCA
  GTTAGCTCAGTATCACGCCAAGGACCCCAATAACCTCTTCATGGTGCGCTTGGCACAGGGCCTGACACAT
  TTAGGGAAGGGCACACTTACCCTCTGCCCCTACCACAGTGACCGGCAGCTCATGAGTCAAGTGGCTGTGG
  CGGGGCTGCTCACCGTGCTTGTTTCTTTCCTGGATGTGCGAAACATCATTCTAGGGAAATCTCACTATGT
  ACTGTATGGGCTGGTGGCCGCCATGCAGCCTCGGATGCTGGTTACATTTGATGAGGAGTTGCGACCATTG
  CCAGTTTCTGTCCGCGTGGGTCAGGCAGTGGATGTGGTGGGCCAGGCTGGGAAGCCCAAAACGATCACGG
  GGTTCCAGACTCACACAACTCCAGTGCTGTTGGCCCATGGGGAACGGGCGGAGCTGGCCACTGAGGAGTT
  TCTCCCTGTCACCCCAATTCTGGAAGGCTTTGTCATTCTTCGAAAGAACCCCAACTACAATCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209024 representing NM_001031639
  Red=Cloning site Green=Tags(s)
  
  MEEGGRDKTPVQSQQPSATAPSGADEKSSGKERRDAGEKDKEQELSEEDKQLQDELEMLVERLGEKDTSL
  YRPALEELRRQIRSSTTSMTSVPKPLKFLRPHYGKLKEIYENMAPGENKCFAADIISVLAMTMSGERECL
  KYRLVGSQEELASWGHEYVRHLAGEVAKEWQELDDAEKAQREPLLTLVKEIVPYNMAHNAEHEACDLLME
  IEQVDMLEKDIDENAYSKVCLYLTSCVNYVPEPENSALLRCALGVFRKFSRFPEALRLALMLNDMELVED
  IFTSCKDVVVQKQMAFMLGRHGVFLELSEDVEEYEDLTEIMSNVQLNSNFLALARELDIMEPKVPDDIYK
  THLENNRFGGSGSQVDSARMNLASSFVNGFVNAAFGQDKLLTDDGNKWLYKNKDHGMLSAAASLGMILLW
  DVDGGLTQIDKYLYSSEDYIKSGALLACGIVNSGVRNECDPALALLSDYVLHNSNTMRLGSIFGLGLAYA
  GSNREDVLTLLLPVMGDSKSSMEVAGVTALACGMIAVGSCNGDVTSTILQTIMEKSETELKDTYARWLPL
  GLGLNHLGKGEAIEAILAALEVVSEPFRSFANTLVDVCAYAGSGNVLKVQQLLHICSEHFDSKEKEEDKD
  KKEKKDKDKKEAPADMGAHQGVAVLGIALIAMGEEIGAEMALRTFGHLLRYGEPTLRRAVPLALALISVS
  NPRLNILDTLSKFSHDADPEVSYNSIFAMGMVGSGTNNARLAAMLRQLAQYHAKDPNNLFMVRLAQGLTH
  LGKGTLTLCPYHSDRQLMSQVAVAGLLTVLVSFLDVRNIILGKSHYVLYGLVAAMQPRMLVTFDEELRPL
  PVSVRVGQAVDVVGQAGKPKTITGFQTHTTPVLLAHGERAELATEEFLPVTPILEGFVILRKNPNYNL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001031639
• ORF Size: 2724 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001031639.1, NP_001026809.1
• RefSeq Size: 2920 bp
• RefSeq ORF: 2727 bp
• Locus ID: 287984
• Cytogenetics: 11q23
• MW: 100.2 kDa