Atp5f1a (NM_023093) Rat Tagged ORF Clone

CAT#: RR209183

Atp5a1 (Myc-DDK-tagged ORF) - Rat ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle (Atp5a1), nuclear gene encoding mitochondrial protein, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Atp5f1a
• Synonyms: Atp5a1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209183 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGTCCGTGCGCATCGCCGCGGCCGTGGCCCGTGCCCTCCCTCGACGGGCGGGACTGGTCTCCAAAA
  ATGCCTTGGGGTCATCTTTTGTTGGTACAAGAAATCTCCATGCCTCTAACACTCGACTTCAGAAGACTGG
  CACTGCCGAGATGTCCTCCATTCTCGAGGAGCGGATCCTTGGAGCTGATACTTCTGTTGACCTTGAAGAG
  ACTGGCCGTGTCTTAAGCATTGGTGATGGTATTGCCCGAGTGCATGGACTGAGGAACGTTCAAGCTGAGG
  AGATGGTAGAGTTTTCTTCCGGCTTAAAGGGTATGTCCCTGAACTTGGAACCCGACAATGTTGGAGTTGT
  CGTGTTTGGGAATGACAAGCTAATTAAAGAAGGTGATATCGTGAAGAGAACAGGAGCCATCGTGGACGTT
  CCAGTTGGCGATGAACTGTTGGGCCGTGTAGTTGATGCCCTGGGAAATGCCATTGATGGGAAGGGTCCAG
  TTGGTTCCAAGATTCGCAGACGAGTGGGCCTGAAAGCCCCTGGAATTATCCCCCGAATCTCTGTGCGGGA
  ACCAATGCAGACGGGCATCAAGGCTGTGGATAGCCTGGTGCCGATTGGCCGAGGTCAGCGTGAGCTGATT
  ATTGGAGACAGACAGACCGGGAAAACCTCGATTGCTATTGACACAATCATCAACCAGAAGCGTTTCAATG
  ATGGGACTGACGAGAAGAAGAAACTGTACTGCATCTACGTTGCTATTGGTCAGAAACGGTCCACCGTTGC
  TCAGTTGGTGAAGAGACTGACAGACGCAGATGCCATGAAGTACACCATTGTGGTGTCAGCCACTGCCTCT
  GATGCTGCCCCGCTTCAGTACTTGGCTCCTTACTCCGGCTGCTCCATGGGAGAGTATTTCCGAGATAATG
  GCAAGCACGCTCTGATCATCTATGACGACTTATCCAAGCAGGCTGTTGCTTACCGCCAGATGTCTCTGCT
  GCTCCGCCGACCCCCGGGTCGAGAGGCCTATCCCGGTGATGTGTTTTACCTACACTCTCGCCTGCTGGAG
  AGAGCAGCCAAGATGAACGATTCCTTTGGTGGTGGCTCTTTGACTGCCTTACCAGTCATTGAAACACAGG
  CTGGTGATGTGTCCGCCTACATTCCAACAAACGTTATTTCCATCACCGATGGACAGATCTTCTTGGAAAC
  AGAATTGTTCTATAAAGGCATCCGCCCTGCCATTAATGTGGGCTTGTCTGTGTCCCGTGTCGGATCTGCC
  GCCCAGACCAGAGCCATGAAGCAGGTGGCAGGCACCATGAAGCTGGAGTTGGCCCAGTACCGGGAGGTCG
  CTGCTTTTGCCCAGTTTGGTTCTGATCTGGATGCTGCCACTCAGCAGCTCTTGAGCCGTGGCGTGCGCCT
  GACCGAGCTGCTAAAGCAAGGACAGTACTCTCCCATGGCTATTGAAGAACAGGTGGCTGTCATCTATGCA
  GGCGTCCGGGGTTATCTTGATAAACTGGAGCCCAGTAAGATCACAAAGTTCGAGAGTGCTTTCTTGTCTC
  ATGTTGTGAGCCAGCACCAGAGCCTCTTGGGCAATATCAGGTCTGATGGGAAAATCTCAGAACAGTCGGA
  TGCAAAGCTGAAGGAAATCGTAACAAACTTCTTGGCTGGGTTTGAACCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209183 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLSVRIAAAVARALPRRAGLVSKNALGSSFVGTRNLHASNTRLQKTGTAEMSSILEERILGADTSVDLEE
  TGRVLSIGDGIARVHGLRNVQAEEMVEFSSGLKGMSLNLEPDNVGVVVFGNDKLIKEGDIVKRTGAIVDV
  PVGDELLGRVVDALGNAIDGKGPVGSKIRRRVGLKAPGIIPRISVREPMQTGIKAVDSLVPIGRGQRELI
  IGDRQTGKTSIAIDTIINQKRFNDGTDEKKKLYCIYVAIGQKRSTVAQLVKRLTDADAMKYTIVVSATAS
  DAAPLQYLAPYSGCSMGEYFRDNGKHALIIYDDLSKQAVAYRQMSLLLRRPPGREAYPGDVFYLHSRLLE
  RAAKMNDSFGGGSLTALPVIETQAGDVSAYIPTNVISITDGQIFLETELFYKGIRPAINVGLSVSRVGSA
  AQTRAMKQVAGTMKLELAQYREVAAFAQFGSDLDAATQQLLSRGVRLTELLKQGQYSPMAIEEQVAVIYA
  GVRGYLDKLEPSKITKFESAFLSHVVSQHQSLLGNIRSDGKISEQSDAKLKEIVTNFLAGFEP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_023093
• ORF Size: 1659 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_023093.1, NP_075581.1
• RefSeq Size: 1871
• RefSeq ORF: 1662
• Locus ID: 65262
• MW: 59.8 kDa
• Gene Summary: mediates production of the majority of cellular ATP; binds ATP and ADP [RGD, Feb 2006]