Abi3 (NM_001013118) Rat Tagged ORF Clone

CAT#: RR209365

Abi3 (Myc-DDK-tagged ORF) - Rat ABI family, member 3 (Abi3), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Abi3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209365 representing NM_001013118
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGCTGCAGCAGCTGCAGCAGTTGCAGGAGTTCGATATCCCCACGGGCCGGGAGGCTCTGCGGG
  GCAACCACAGCGCCCTGCTACGGGTGGCCAACTACTGTGAGGATAACTACTTGCAGGCCACAGACAAGCG
  GAAGGCGCTGGAAGAGACGATGGCTTTCACCACCCAGGCCCTGGCCAGTGTAGCCTATCAAGTGGGTAAC
  CTGGCGGGGCACACGCTTCGAATGCTGGATCTACAGGGTGCTGCCCTGCGGCAGGTGGAAGCCAAGATGA
  GCACACTGGGCCAGATGGTGAACATGCACCTGGAGAAAGTAGCCAGAAGGGAGATTGGCACGTTGGCCAC
  TGTCGTGCGGCTGCCCCCTAGCCAGAAGGTCATCCCTCCTGAGAGCCTGCCTCCCCTCACTCCCTACTGC
  AGAAAACCCCTCAACTTTGCCTGCTTGGATGATGTTGGCCATGGAGTCAAGGACTTGAGCACACAGCTGT
  CACGGACCGGGACCCTGTCTCGCAAGAGCATAAAGGCGCCCGCTACACCTGCCTCTGCCACGCTGGGGAG
  ACCACCCCGGATCCCTGAGCCGGTGCAGCTCCCAGCGGTGCCAGACGGCAAGCTCTCCGCTGCCTCCTCT
  GTGTCTTCCTTGGCCTCCGCAGGCAGTGCAGAAGGTGCCAGTGGGATCCCCCAGTCCAAGGGACAGGTAG
  CACCTGCAACCCCGCCTCCTCCACCTATAGCGCCTGTAACTCCACCTCCTCCACCATTGCCTGCTGAGAT
  CTTCTTGCTGCCCCCTCCGATGGAGGAGTCCCAGCCCCCTCCGGAAACAGAGTTGCCCCTGCCTCCTCCT
  CCGGCTCTACAGGGGGATGAACTGGGGCTGCTGCCTCCGCCTCCACCAGGTTTTGGACCGGATGAGCCCA
  GCTGGGTCCCTGCTGCCTACTTGGAGAAAGTGGTGACGCTGTACCCATACACCCGGCAGAAGGACAATGA
  GCTCTCCTTTTCTGAAGGAACCGTCATCTGTGTCACTCGACGCTACTCAGATGGCTGGTGTGAGGGTGTC
  AGCTCAGAGGGCACTGGATTCTTCCCAGGGAACTATGTGGAGCCCAGCTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209365 representing NM_001013118
  Red=Cloning site Green=Tags(s)
  
  MAELQQLQQLQEFDIPTGREALRGNHSALLRVANYCEDNYLQATDKRKALEETMAFTTQALASVAYQVGN
  LAGHTLRMLDLQGAALRQVEAKMSTLGQMVNMHLEKVARREIGTLATVVRLPPSQKVIPPESLPPLTPYC
  RKPLNFACLDDVGHGVKDLSTQLSRTGTLSRKSIKAPATPASATLGRPPRIPEPVQLPAVPDGKLSAASS
  VSSLASAGSAEGASGIPQSKGQVAPATPPPPPIAPVTPPPPPLPAEIFLLPPPMEESQPPPETELPLPPP
  PALQGDELGLLPPPPPGFGPDEPSWVPAAYLEKVVTLYPYTRQKDNELSFSEGTVICVTRRYSDGWCEGV
  SSEGTGFFPGNYVEPSC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001013118
• ORF Size: 1101 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001013118.1, NP_001013136.1
• RefSeq Size: 1708 bp
• RefSeq ORF: 1104 bp
• Locus ID: 303476
• Cytogenetics: 10q26
• MW: 39.1 kDa