Prtg (NM_001037651) Rat Tagged ORF Clone

CAT#: RR209418

Prtg (Myc-DDK-tagged ORF) - Rat protogenin homolog (Gallus gallus) (Prtg), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Prtg
• Synonyms: RGD1307157
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209418 representing NM_001037651
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCCTCCCGTGCGCCCGGGGATGCTGCCCCTGCTGCTGTTGCTGCTACTGCCGCCGCTCGGCTCGG
  TTCCAGGAGTGTGGAGCTTTAGCGAACTGTTCTTCATGAAAGAACCACAGGACGCAACGGTCACAAGGAA
  AGACCCAGTCTTCTTAGATTGCCAGGCCCATGGAGAAGGTCCCATTAAGGTCACCTGGTTGAAAAATGGA
  GCAAAATTGTCTGAAAATAAACGGATCCAGGTTCTATCTAACGGCTCTTTATACATCAGTGAGGCGGAAG
  GCAGGCGGGGAGAGCAATCGGATGAAGGATTTTATCAGTGCTTGGCTGTAAACAAATATGGGGCCATTCT
  TAGTCAGAAAGCTCATCTGACATTGTCAACCATCTCTGCGTTCGAGGTCCATCCAGTGTCTACCGAAGTC
  CCCGAAGGGGGAGTTGCTAGATTTTCATGCAAGATTTCATCAACGCCCCCTGCGGTCATAACATGGGAAT
  TTAATAGGACAGCCCTGCCTATGACCATGGACAGCAGGGTGACTGCCCTGCCCTCAGGAGTGCTGCAGAT
  CTATGACGCTGGCCCGGAGGATGCTGGGAAATACCGCTGTGTTGCAGCCACGCATGCTCATAAGCGTAAA
  AGCATGGAGGCCTCGCTAACCATAGTTCCAGCTAATGAGACCAGATCCTTCTACATGCCCACCATTATAG
  CCAGTCCACAGAATGTAACCGCGTCTCTACATCAGACAGTTGTGCTAGAGTGTATGGCTACAGGATATCC
  CAAGCCCATCATTTCCTGGAGCCGTCTTGATCACAAGTCCATTGATGTCTTCAATACTCGGGTACTCGGA
  AATGGCAATCTCATCATATCAGATGTCAAGCTTCAGCATGCTGGAGTGTATGTTTGTCGGGCCACCACCC
  CAGGCACTCGCAACTTCACAGTTGCTATGGCAACGTTAACTGTATTAGCTCCTCCTTCATTTGTGGAATG
  GCCTGAAAGTTTAACAAGGCCACGAGCTGGCACTGCACGGTTTGTGTGTCAAGCAGAAGGAATTCCCTCA
  CCCAAAATGTCCTGGTTGAAAAATGGGAGAAGGATACATTCAAATGGCAGGATTAAAATGTACAACAGCA
  AATTGGTCATTAACCAGATTATCCCAGAAGATGATGCTATCTATCAGTGCATGGCTGAAAACAGCCAAGG
  ATCTGTTCTGTCTCGAGCCAGACTGACTGTGGTGATGTCGGAAGATAGACCCAGCGCTCCTTACAACGTT
  CATGCCGAAACCATGTCGAGCTCTGCTATCCTTCTAGCATGGGAGCGACCACTGTACAATTCAGACAAGG
  TCATTGCCTACTCTGTACACTACATGAAGGCTGAAGGTCTAAATAACGAGGAATATCAGGTGGTCCTTGG
  AAATGACACAACTCATTATATCATCGATGACTTGGAACCAGACAGCAACTATACTTTCTACATCGTGGCG
  TACATGCCCATGGGAGCCAGCCAGATGTCGGATCATGTGACACAGAACACGCTAGAAGACGTGCCCCTAA
  GGCCTCCCGAAATTAGCTTGACGAGTCGAAGTCCCACCGACATTCTCATCTCCTGGCTGCCCATCCCGGC
  CAAGTACCGACGGGGCCAAGTGGTGCTGTACCGGCTGTCCTTCCGCCTGAGCACTGAGAATTCCATCCAA
  GTTGTAGAACTCCCGGGGACTGTGCACGAGTACCTTCTGGAAGGCCTGGAGCCAGACAGTGTCTATCTGG
  TCCGGATTACTGCTGCTACCCGAGTGGGGCTGGGAGAGTCATCGGTGTGGACGTCACACAGGACGCCCAA
  AGCCACGAGCGTGAAAGCCCCTAAGTCTCCTGAACTGCATTTGGAGCCACTGAATTGTACTACCATCTCT
  GTAAGGTGGCTTCAAGACACCGAGGACCCGGCTGCCATTCGGGGCTACAAGCTGTTTTATAAAGAAGAAG
  GGCAGCAGGAACATGGACCGATCTTCCTGGACACCGGTGACCTGCTGTATACGCTCAGTGGCTTGGACCC
  CAGAAGAAAATACCATGTGAGGCTACTGGCTTACAACAATCTGGAAGATGGCTACCAGGCCGACCAGACC
  GTCAGTACTCCCGGATGCGTGTCTGTCCGTGACCGCATGGTCCCTCCGCCACCGCCACCCCACCATCTGT
  ACGCGAAGGCTAATACCTCCTCTTCTATCTTCCTGCACTGGAGGAGGCCTGCGTTCACCACCGCCCAAGT
  CATTAACTACACCATCCGCTGTAACCCCGTTGGCCTGCAGAACGCCTCTCTGGTTCTCTACCTGCAAACA
  TCAGAAACCCACATGCTGGTTCAAGGACTAGAGCCAAACACCAAGTATGAGTTCGCTGTCCGACTGCACG
  TGGATCAGCTCTCCAGTCCCTGGAGTCCCGTGGTCTACCACACCACACTGCCGGAAGCACCCACAGGCCC
  CCCAGTGGGGGTGAAGGTGACGCTGATAGAAGATGACACTGCCCTTGTCTCTTGGAAACCCCCAGATGGC
  CCAGAGACAGTGGTGACGCGCTACACCATCTTGTATGCGTCCAGGAAGGCCTGGATCGCCGGCGAGTGGC
  AGGTCCTACATCGAGAAGGGGCAATAACCATGGCTCTGCTGGAAAACCTGGTGGCAGGAAACGTGTACAT
  TGTCAAGATCTCAGCCTCCAACGAGGTGGGAGAAGGGCCCTTCCCAAATTCCGTGGAGCTGGCCGTCCTC
  CCCAAGGACGCTTCAGAATCGAACCAGAGGCCTAAGCGTTTGGATTCTTCTGATGCCAAAGTTTATTCAG
  GCTATTACCACCTGGACCAAAAGTCAATGACCGGCATTGCAGTAGGCGTTGGCATAGCCTTGACCTGTAT
  CCTCATCTGTGTTCTCATCTTGATATACCGAAGCAAAGCCAGGAAGTCATCCGCCTCTAAGACAACGCAG
  AGTGGAACCCAGCCGTTATCCCGAGCCAGTGCCTCTGTAGCAGCGGGGAGTGACATGGGGAAGAACCTGG
  AGAGAGCTACAGAAAACGAAGAGTCCTCAGTACCCATGATGCCAAGCTGCTTCATTGATGCGAAGGGAGG
  GACTGACCTGATCATCAATAGCTATGGTCCTATTATTAAAAACAACCCTAAGAAAAAGTGGCGTTTTTTC
  CAAGACACTAAGAAGATCAAAGTTGAACAGACTCAAAGAAGAATTACTCAGACCGTGTGCTTTTACCAGC
  CAGGCACCACAGTGCTGATCAGCGATGAAGACTCCCCTAGCTCCCCAGGCCAGACCACCAGCTTCCCAAG
  ACCTTTTGGGCCCACCACCCTGGACACTGAGCACTCAGCAAACAGTGAAGGCAGCCACGAGACAGGGGAT
  TCTGGAAGGTTTTCCCATGAGTCCAATGATGAGATACATCTGTCCTCTGTCATCAGCAGCACACCCCCCA
  CCTCGAATTCCCTCACCTGTGGTGATTCTGATGGGGATGCTGCACCGAAGAAGCATGGAGACCCTGCCCA
  GCCCTTGCCAGCTGAGCAGACCTCAGCCCCACAGCCAACACCTGCTGGGCTGCGACATGCTGCCGAGAGT
  GTTCCCGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209418 representing NM_001037651
  Red=Cloning site Green=Tags(s)
  
  MAPPVRPGMLPLLLLLLLPPLGSVPGVWSFSELFFMKEPQDATVTRKDPVFLDCQAHGEGPIKVTWLKNG
  AKLSENKRIQVLSNGSLYISEAEGRRGEQSDEGFYQCLAVNKYGAILSQKAHLTLSTISAFEVHPVSTEV
  PEGGVARFSCKISSTPPAVITWEFNRTALPMTMDSRVTALPSGVLQIYDAGPEDAGKYRCVAATHAHKRK
  SMEASLTIVPANETRSFYMPTIIASPQNVTASLHQTVVLECMATGYPKPIISWSRLDHKSIDVFNTRVLG
  NGNLIISDVKLQHAGVYVCRATTPGTRNFTVAMATLTVLAPPSFVEWPESLTRPRAGTARFVCQAEGIPS
  PKMSWLKNGRRIHSNGRIKMYNSKLVINQIIPEDDAIYQCMAENSQGSVLSRARLTVVMSEDRPSAPYNV
  HAETMSSSAILLAWERPLYNSDKVIAYSVHYMKAEGLNNEEYQVVLGNDTTHYIIDDLEPDSNYTFYIVA
  YMPMGASQMSDHVTQNTLEDVPLRPPEISLTSRSPTDILISWLPIPAKYRRGQVVLYRLSFRLSTENSIQ
  VVELPGTVHEYLLEGLEPDSVYLVRITAATRVGLGESSVWTSHRTPKATSVKAPKSPELHLEPLNCTTIS
  VRWLQDTEDPAAIRGYKLFYKEEGQQEHGPIFLDTGDLLYTLSGLDPRRKYHVRLLAYNNLEDGYQADQT
  VSTPGCVSVRDRMVPPPPPPHHLYAKANTSSSIFLHWRRPAFTTAQVINYTIRCNPVGLQNASLVLYLQT
  SETHMLVQGLEPNTKYEFAVRLHVDQLSSPWSPVVYHTTLPEAPTGPPVGVKVTLIEDDTALVSWKPPDG
  PETVVTRYTILYASRKAWIAGEWQVLHREGAITMALLENLVAGNVYIVKISASNEVGEGPFPNSVELAVL
  PKDASESNQRPKRLDSSDAKVYSGYYHLDQKSMTGIAVGVGIALTCILICVLILIYRSKARKSSASKTTQ
  SGTQPLSRASASVAAGSDMGKNLERATENEESSVPMMPSCFIDAKGGTDLIINSYGPIIKNNPKKKWRFF
  QDTKKIKVEQTQRRITQTVCFYQPGTTVLISDEDSPSSPGQTTSFPRPFGPTTLDTEHSANSEGSHETGD
  SGRFSHESNDEIHLSSVISSTPPTSNSLTCGDSDGDAAPKKHGDPAQPLPAEQTSAPQPTPAGLRHAAES
  VPV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001037651
• ORF Size: 3579 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001037651.1, NP_001032740.1
• RefSeq Size: 5326 bp
• RefSeq ORF: 3582 bp
• Locus ID: 315806
• Cytogenetics: 8q24
• MW: 131.1 kDa