Ippk (NM_001008556) Rat Tagged ORF Clone

CAT#: RR209419

Ippk (Myc-DDK-tagged ORF) - Rat inositol 1,3,4,5,6-pentakisphosphate 2-kinase (Ippk), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ippk
• Synonyms: C9orf12; RGD1311271; rIpk1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209419 representing NM_001008556
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGAGGGGAAAATGGACGAGAATGAATGGAGTTACCATGGCGAAGGCAACAAGAGCCTTGTGGTGG
  CCCACGCGCAGCGCTGTGTAGTTCTGCGCTTTCTGAAGTTTCCTCCAAATAAGAAGAAGACCTCAGAAGA
  GATACTTCAGCATCTGCAGAACATAGTGGACTTTGGCAAAAATGTCATGAAAGACTTTTTGGGAGAGAAC
  TATGTTCATTGTGGGGAGGTTGTTCAGCTGCCTTTAGAGTTTGTGAAACAACTGTGTTTAAAGATACAGT
  GTGAAAGACCAGAGTCCCGTTGTGACAAGGACCTGGACACACTCAGTGGTTATGCTATGTGCCTTCCCAA
  TTTGACCAGACTTCAGACCTTCCCCTTTGCAGAGCACCGACCAATTCTGTGTGTGGAGATTAAGCCAAAA
  TGTGGGTTTATTCCTTTCTCAAATGGTGTCACCCACGAGATGAAGCACAAGGTGTGCCGGTACTGCATGC
  ACCAGCACCTCAAGGTAGCGACTGGAAAGTGGAAGAAAATCAGTAAATACTGTCCCCTTGACCTCTACTC
  AGGAAATAAGCAGCGTATGCACTTTGCCTTGAAGAGTCTTCTGCAGGAGGCGCAGAATAACCTTAGGATA
  TTTAAGAATGGAGAGCTGATTTATGGCTGTGCTGATGCCCGGAGCCCCGTGGCTGACTTGAAGGCGCTTG
  CACACCACCTGAAGCCCTTCTTCTTCCCTTCCAATGGCCTGGCCAGTGGGCCTCAGTGCACGAGGGCTGT
  GATCCGGGAGCTGGTGCATGTTATCACCAGGGTGCTGCTCAGCACCTCAGACAAAGGCCGAGCGGGTGCC
  TTGAGGCTTGGGCTCCAGGGCGCACGAGTCTGTGAAGCCAGCCCATTCAGCAGGAGCCTACATCACCAAG
  GAAAAAACACCCCAGAGCACTCGGGGTTACCGAAGGGCTGTCTTCTGTACAAAACCCTCCAGGTGCAGAT
  GTTGGACCAGCTGGACATCGAAGGCCTCTACCCTCTGTACAACCGGGTTGAGCAATACCTGGAGGAGTTT
  CCTGAGGAGAGGAAAACATTACAGATAGATGGGCCTTATGATGAAGTATTTTACCAGAAACTGCTTGATC
  TATCCACTGAGGATGATGGGACAGTGGCCTTTGCATTAACAAAGGTGCAGCAGTACCGAGTAGCCATGAC
  CGCCAAGGACTGTTCCATCATGATCGCACTTTCCCCTTGTCTACAGGGCGCCAGCTCTGACCAGAGGCCT
  GTTATCCCTTCGTCAAGATCCCGGCTGGCCTTCTCTGTGTCTGTACTGGACCTCGACCTCAAGCCCTATG
  AGAGCATTCCTCACCAGTATAAACTAGACAGCAAGATAGTCAGCTATTACTCAAAGACTGTGCATGCCAA
  AGATGACACTGTGAGGTCCACTCGGTTCAAGGAACATGAAGACTGCACATTAGTTCTCCATAAGGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209419 representing NM_001008556
  Red=Cloning site Green=Tags(s)
  
  MEEGKMDENEWSYHGEGNKSLVVAHAQRCVVLRFLKFPPNKKKTSEEILQHLQNIVDFGKNVMKDFLGEN
  YVHCGEVVQLPLEFVKQLCLKIQCERPESRCDKDLDTLSGYAMCLPNLTRLQTFPFAEHRPILCVEIKPK
  CGFIPFSNGVTHEMKHKVCRYCMHQHLKVATGKWKKISKYCPLDLYSGNKQRMHFALKSLLQEAQNNLRI
  FKNGELIYGCADARSPVADLKALAHHLKPFFFPSNGLASGPQCTRAVIRELVHVITRVLLSTSDKGRAGA
  LRLGLQGARVCEASPFSRSLHHQGKNTPEHSGLPKGCLLYKTLQVQMLDQLDIEGLYPLYNRVEQYLEEF
  PEERKTLQIDGPYDEVFYQKLLDLSTEDDGTVAFALTKVQQYRVAMTAKDCSIMIALSPCLQGASSDQRP
  VIPSSRSRLAFSVSVLDLDLKPYESIPHQYKLDSKIVSYYSKTVHAKDDTVRSTRFKEHEDCTLVLHKV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001008556
• ORF Size: 1467 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001008556.1, NP_001008556.1
• RefSeq Size: 1470 bp
• RefSeq ORF: 1470 bp
• Locus ID: 306808
• Cytogenetics: 17p14
• MW: 55.6 kDa