Eif2ak1 (NM_013223) Rat Tagged ORF Clone

CAT#: RR209421

Eif2ak1 (Myc-DDK-tagged ORF) - Rat eukaryotic translation initiation factor 2 alpha kinase 1 (Eif2ak1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Eif2ak1
• Synonyms: Hri
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209421 representing NM_013223
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGGGGGCGGCTCCGTGGATGGCGAGCGCGACACGGACGACGACGCGGCGGGGGCGGTGGCCGCGC
  CTCCTGCCATCGACTTCCCCGCAGAGGTGTCGGACCCCAAGTATGATGAGTCCGATGTCCCAGCAGAGCT
  CCAAGTGTTCAAAGAGCCCTTGCAGCAGCCCACGTTCCCTTTCTTAGTGGCAAACCAGCTGCTGCTGGTC
  TCCTTGCTGGAACACTTGAGCCATGTGCACGAGCCGAACCCACTTCACTCAAAACAGGTCTTTAAATTAC
  TGTGCCAGACTTTTATCAAGATGGGGCTGCTCTCTTCCTTTACCTGCAGCGATGAGTTCAGCTCCCTGAG
  ACTCCACCACAACAGAGCCATCACTCATTTAATGAGGTCTGCCAAGGAGAGAGTCCGTCAGGATCCTTGT
  CAAGATAATTCTTACATGCAGAAAATCAGATCCAGGGAGATAGCTCTCGAAGCACAGACTTCACGCTACT
  TAAATGAATTTGAAGAGCTCGCCATCTTAGGAAAAGGCGGATATGGAAGAGTTTACAAGGTCCGGAACAA
  ATTAGATGGTCAGCATTATGCAATTAAGAAAATCCTGATTAAGAGTGCAACTAAAACGGATTGTATGAAG
  GTGCTACGGGAAGTGAAGGTTCTGGCAGGCCTCCAGCACCCCAATATCGTTGGCTACCACACTGCATGGA
  TAGAGCACGTTCACGTGCTTCAGCCACAAGACAGAGTTCCCATTCAACTGCCCTCTCTTGAAGTGTTGTC
  AGAGCATGAAGGGGACAGAAATCAAGGTGGTGTTAAAGATAATGAAAGCAGTTCGTCCATTATCTTTGCT
  GAACTCACCCCAGAAAAAGAAAACCCTTTAGCGGAATCTGATGTTAAGAATGAGAATAACAACTTGGTGA
  GCTACAGGGCCAACTTAGTGATCAGGAGCAGCAGTGAAAGCGAATCGTCCATTGAGCTCCAAGAAGATGG
  CTTGAACGAGTCGCCTCTCAGACCAGTTGTCAAGCACCAGCTGCCGCTGGGGCATAGCTCAGACGTGGAA
  GGGAATTTTACCTCCACGGATGAGTCTTCTGAAGACAATTTGAACCTGCTGGGGCAGACAGAGGCGCGGT
  ACCACCTGATGCTGCACATCCAGATGCAGCTGTGTGAGCTCTCCCTGTGGGACTGGATAGCTGAGAGGAA
  CAAGCGGAGCCGGAAGTGCGTGGATGAAGCAGCTTGTCCCTATGTTATGGCCAGTGTTGCAACAAAGATT
  TTTCAAGAACTGGTGGAAGGTGTCTTTTACATACATAACATGGGCATCGTCCACAGAGATCTGAAGCCTA
  GAAATATTTTTCTTCATGGTCCTGATCAACAAGTGAAAATAGGAGACTTTGGTCTGGCCTGTGCAGACAT
  CATTCAGAAGAGCGCAGATTGGACCAACAGAAACGGGAAAGGAACGCCGACACACACGTCCCGAGTGGGG
  ACTTGTCTCTACGCGTCACCTGAGCAGTTGGAAGGATCCGAGTATGATGCCAAGTCTGATATGTACAGCT
  TGGGTGTGATCCTGCTCGAGCTCTTCCAGCCATTCGGGACAGAAATGGAGCGAGCAACAGTCCTAACAGG
  TGTGAGGACTGGTCGGATACCAGAGTCCCTCAGTAAAAGGTGTCCAGTGCAAGCCAAGTATATCCAGCTC
  CTGACTGGGAGGAACGCGGCCCAGAGACCATCTGCTCTTCAGCTACTGCAGAGCGAACTGTTCCAAACAA
  CGGGAAATGTTAATCTCACATTGCAGATGAAGATAATGGAGCAAGAAAAGGAAATTGAAGAATTAAAGAA
  GCAACTAAGCCTCCTTTCGCAGGACAAAGGGCTGAAGAGA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209421 representing NM_013223
  Red=Cloning site Green=Tags(s)
  
  MLGGGSVDGERDTDDDAAGAVAAPPAIDFPAEVSDPKYDESDVPAELQVFKEPLQQPTFPFLVANQLLLV
  SLLEHLSHVHEPNPLHSKQVFKLLCQTFIKMGLLSSFTCSDEFSSLRLHHNRAITHLMRSAKERVRQDPC
  QDNSYMQKIRSREIALEAQTSRYLNEFEELAILGKGGYGRVYKVRNKLDGQHYAIKKILIKSATKTDCMK
  VLREVKVLAGLQHPNIVGYHTAWIEHVHVLQPQDRVPIQLPSLEVLSEHEGDRNQGGVKDNESSSSIIFA
  ELTPEKENPLAESDVKNENNNLVSYRANLVIRSSSESESSIELQEDGLNESPLRPVVKHQLPLGHSSDVE
  GNFTSTDESSEDNLNLLGQTEARYHLMLHIQMQLCELSLWDWIAERNKRSRKCVDEAACPYVMASVATKI
  FQELVEGVFYIHNMGIVHRDLKPRNIFLHGPDQQVKIGDFGLACADIIQKSADWTNRNGKGTPTHTSRVG
  TCLYASPEQLEGSEYDAKSDMYSLGVILLELFQPFGTEMERATVLTGVRTGRIPESLSKRCPVQAKYIQL
  LTGRNAAQRPSALQLLQSELFQTTGNVNLTLQMKIMEQEKEIEELKKQLSLLSQDKGLKR
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013223
• ORF Size: 1860 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_013223.1, NP_037355.1
• RefSeq Size: 2145
• RefSeq ORF: 1863
• Locus ID: 27137
• MW: 69.6 kDa
• Gene Summary: protein kinase, negatively regulated by hemin; may play an important role in translational control of protein synthesis [RGD, Feb 2006]