Dclk2 (NM_001009691) Rat Tagged ORF Clone

CAT#: RR209497

Dclk2 (Myc-DDK-tagged ORF) - Rat doublecortin-like kinase 2 (Dclk2), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dclk2
• Synonyms: CL2; CLICK-II; CLICK2; Dck2; RGD1308384
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209497 representing NM_001009691
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGCACCAGGAGCATTGAGCTGGAACATTTTGAAGAACGGGACAAAAGGCCGCGGCCAGGGTCGC
  GGAGAGGAGCTCCCAGCTCCTCCGGGGGCAGCAGCATCTCTGGCCCCAAGGGCAACGGGCTCATCCCCAG
  CCCGGCGCACAGTGCTCACTGCAGCTTCTACCGCACGCGGACCTTGCAGGCCCTCAGCTCGGAGAAGAAG
  GCCAAGAAGGCGCGGTTCTACCGGAATGGGGACCGCTACTTCAAAGGCCTGGTCTTTGCGATCTCCAGCG
  ACCGTTTCCGTTCCTTCGATGCGCTCCTCATAGAGCTCACCCGCTCGCTGTCTGACAATGTGAACCTGCC
  CCAGGGCGTCCGCACAATCTACACCGTAGACGGCAGCCGGAAGGTCACCAGCCTGGACGAGCTGCTGGAA
  GGTGAAAGTTACGTGTGTGCCTCCAACGAACCATTCCGTAAAGTTGACTACACCAAAAACGTTAACCCGA
  ACTGGTCTGTGAACATCAAGGGTGGTACTACCCGCACCTTGGCCGTGGCCTCGGCCAAGAGTGAGGTGAA
  AGAAAGCAAGGACTTCATCAAACCCAAGCTCGTGACTGTGATTCGAAGTGGGGTGAAGCCTAGAAAGGCC
  GTGCGCATCCTTCTGAATAAAAAGACTGCCCATTCCTTTGAGCAGGTCCTAACGGATATCACCGAAGCCA
  TTAAACTAGACTCCGGCGTGGTGAAGAGGCTGTGCACCCTGGATGGAAAGCAGGTTACTTGTCTGCAAGA
  CTTTTTTGGAGACGATGATGTTTTTATTGCATGTGGACCTGAAAAATATCGTTATGCCCAAGATGACTTT
  GTCCTGGATCATAGTGAGTGCCGTGTCCTGAAGTCATCTTATTCTCGGGCCTCAGCTGCTAAGTATTCTG
  GATCCAGAAGCCCAGGGCTCTCCCGCCGCAGCAAGTCACCAGCTTCAGTAAAGAGGGCTGGCCACTCCAG
  TGCCTATTCTACAGCCAAATCCCCAGTGAATGGAACTCCCAGCAGCCAGCTTTCCACTCCTAAGTCCACC
  AAGTCCTCCAGCTCCTCTCCAACCAGCCCGGGAAGTTTCAGAGGACTGAAGCAGATTTCTGCTCAGGGCA
  GATCTTCTTCCAATGTAAACGGTGGGCCCGAACTTGACCGTTGCATGAGTCCTGAAGGCGTGAATGGAAA
  CAGGTGCTCCGAGTCATTCACTCTTCTGGAGAAATACAGAATCGGGAAGGTCATCGGAGACGGCAACTTT
  GCCGTAGTTAAAGAATGTATGGACAGGTCCACTGGAAAGGAGTTTGCATTAAAGATTATTGACAAAGCCA
  AATGCTGTGGAAAGGAACATCTGATTGAGAACGAAGTGTCAATCCTGCGCCGGGTGAAGCACCCCAACAT
  CATCATGCTGGTTGAGGAGATGGAAACAACAACTGAGCTCTTCCTAGTGATGGAACTGGTCAAAGGTGGA
  GATCTCTTCGACGCGATCACTTCTTCAACCAAGTACACCGAGAGAGATGGCAGTGCCATGGTGTACAACC
  TAGCCAGCGCCCTCAGATACCTGCACGGCCTCAGCATCGTGCACAGAGATATCAAACCTGAGAATCTGCT
  GGTGTGCGAATACCCAGACGGAACCAAGTCGCTGAAGCTGGGAGACTTTGGGCTGGCGACGGTGGTTGAA
  GGCCCGTTGTACACAGTCTGTGGCACCCCAACTTACGTGGCACCCGAGATCATTGCTGAAACAGGTTACG
  GCCTGAAGGTGGATGTTTGGGCAGCTGGTGTGATTACGTACATTCTTCTCTGTGGATTCCCACCGTTCCG
  GAGTGAGAACAACCTCCAGGAGGATCTCTTTGACCAGATCTTGGCCGGAAAGCTGGAGTTTCCAGCTCCC
  TACTGGGACAACATTACAGACTCTGCCAAGGAGTTAATCAGTCAAATGCTTCAGGTAAACGTTGAAGCTC
  GCTGTACTGCGGGAGAAATTCTGAGTCACCCCTGGGTGTCAGATGATGCATCCCAGGAGAATAATATGCA
  AGCCGAGGTTACAGGTAAACTAAAACAGCACTTTAATAATGCGCTCCCCAAACAGAACAGCACCACCACT
  GGGGTCTCCGTTATCATGAACACGGCTCTAGATAAAGAGGGGCAGGTTTTCTGCAGCAAGCACTGTCGGG
  ACAGCAGCAAATCATCCAGGGAGCAGACCTCAGCCCGGGAGGCCCCGCCCCCACCGGAGTCTCCCAGGCC
  CCCTGGTCCTCCAGCCACCTCTGGCTGTGATCCTGCCGGGACCTGGCGTCGCCATCGAGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209497 representing NM_001009691
  Red=Cloning site Green=Tags(s)
  
  MASTRSIELEHFEERDKRPRPGSRRGAPSSSGGSSISGPKGNGLIPSPAHSAHCSFYRTRTLQALSSEKK
  AKKARFYRNGDRYFKGLVFAISSDRFRSFDALLIELTRSLSDNVNLPQGVRTIYTVDGSRKVTSLDELLE
  GESYVCASNEPFRKVDYTKNVNPNWSVNIKGGTTRTLAVASAKSEVKESKDFIKPKLVTVIRSGVKPRKA
  VRILLNKKTAHSFEQVLTDITEAIKLDSGVVKRLCTLDGKQVTCLQDFFGDDDVFIACGPEKYRYAQDDF
  VLDHSECRVLKSSYSRASAAKYSGSRSPGLSRRSKSPASVKRAGHSSAYSTAKSPVNGTPSSQLSTPKST
  KSSSSSPTSPGSFRGLKQISAQGRSSSNVNGGPELDRCMSPEGVNGNRCSESFTLLEKYRIGKVIGDGNF
  AVVKECMDRSTGKEFALKIIDKAKCCGKEHLIENEVSILRRVKHPNIIMLVEEMETTTELFLVMELVKGG
  DLFDAITSSTKYTERDGSAMVYNLASALRYLHGLSIVHRDIKPENLLVCEYPDGTKSLKLGDFGLATVVE
  GPLYTVCGTPTYVAPEIIAETGYGLKVDVWAAGVITYILLCGFPPFRSENNLQEDLFDQILAGKLEFPAP
  YWDNITDSAKELISQMLQVNVEARCTAGEILSHPWVSDDASQENNMQAEVTGKLKQHFNNALPKQNSTTT
  GVSVIMNTALDKEGQVFCSKHCRDSSKSSREQTSAREAPPPPESPRPPGPPATSGCDPAGTWRRHRD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001009691
• ORF Size: 2301 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001009691.1, NM_001009691.2, NM_001009691.3, NP_001009691.3
• RefSeq Size: 3969 bp
• RefSeq ORF: 2304 bp
• Locus ID: 310698
• Cytogenetics: 2q34
• MW: 84 kDa
• Gene Summary: This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmoduline-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. Mouse studies show that this gene and the DCX gene, another family member, share function in the establishment of hippocampal organization and that their absence results in a severe epileptic phenotype and lethality, as described in human patients with lissencephaly. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Sep 2010]