Dclk2 (NM_001009691) Rat Tagged ORF Clone
CAT#: RR209497
- TrueORF®
Dclk2 (Myc-DDK-tagged ORF) - Rat doublecortin-like kinase 2 (Dclk2), (10 ug)
"NM_001009691" in other vectors (3)
Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Dclk2 |
Synonyms | CL2; CLICK-II; CLICK2; Dck2; RGD1308384 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR209497 representing NM_001009691
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAGCACCAGGAGCATTGAGCTGGAACATTTTGAAGAACGGGACAAAAGGCCGCGGCCAGGGTCGC GGAGAGGAGCTCCCAGCTCCTCCGGGGGCAGCAGCATCTCTGGCCCCAAGGGCAACGGGCTCATCCCCAG CCCGGCGCACAGTGCTCACTGCAGCTTCTACCGCACGCGGACCTTGCAGGCCCTCAGCTCGGAGAAGAAG GCCAAGAAGGCGCGGTTCTACCGGAATGGGGACCGCTACTTCAAAGGCCTGGTCTTTGCGATCTCCAGCG ACCGTTTCCGTTCCTTCGATGCGCTCCTCATAGAGCTCACCCGCTCGCTGTCTGACAATGTGAACCTGCC CCAGGGCGTCCGCACAATCTACACCGTAGACGGCAGCCGGAAGGTCACCAGCCTGGACGAGCTGCTGGAA GGTGAAAGTTACGTGTGTGCCTCCAACGAACCATTCCGTAAAGTTGACTACACCAAAAACGTTAACCCGA ACTGGTCTGTGAACATCAAGGGTGGTACTACCCGCACCTTGGCCGTGGCCTCGGCCAAGAGTGAGGTGAA AGAAAGCAAGGACTTCATCAAACCCAAGCTCGTGACTGTGATTCGAAGTGGGGTGAAGCCTAGAAAGGCC GTGCGCATCCTTCTGAATAAAAAGACTGCCCATTCCTTTGAGCAGGTCCTAACGGATATCACCGAAGCCA TTAAACTAGACTCCGGCGTGGTGAAGAGGCTGTGCACCCTGGATGGAAAGCAGGTTACTTGTCTGCAAGA CTTTTTTGGAGACGATGATGTTTTTATTGCATGTGGACCTGAAAAATATCGTTATGCCCAAGATGACTTT GTCCTGGATCATAGTGAGTGCCGTGTCCTGAAGTCATCTTATTCTCGGGCCTCAGCTGCTAAGTATTCTG GATCCAGAAGCCCAGGGCTCTCCCGCCGCAGCAAGTCACCAGCTTCAGTAAAGAGGGCTGGCCACTCCAG TGCCTATTCTACAGCCAAATCCCCAGTGAATGGAACTCCCAGCAGCCAGCTTTCCACTCCTAAGTCCACC AAGTCCTCCAGCTCCTCTCCAACCAGCCCGGGAAGTTTCAGAGGACTGAAGCAGATTTCTGCTCAGGGCA GATCTTCTTCCAATGTAAACGGTGGGCCCGAACTTGACCGTTGCATGAGTCCTGAAGGCGTGAATGGAAA CAGGTGCTCCGAGTCATTCACTCTTCTGGAGAAATACAGAATCGGGAAGGTCATCGGAGACGGCAACTTT GCCGTAGTTAAAGAATGTATGGACAGGTCCACTGGAAAGGAGTTTGCATTAAAGATTATTGACAAAGCCA AATGCTGTGGAAAGGAACATCTGATTGAGAACGAAGTGTCAATCCTGCGCCGGGTGAAGCACCCCAACAT CATCATGCTGGTTGAGGAGATGGAAACAACAACTGAGCTCTTCCTAGTGATGGAACTGGTCAAAGGTGGA GATCTCTTCGACGCGATCACTTCTTCAACCAAGTACACCGAGAGAGATGGCAGTGCCATGGTGTACAACC TAGCCAGCGCCCTCAGATACCTGCACGGCCTCAGCATCGTGCACAGAGATATCAAACCTGAGAATCTGCT GGTGTGCGAATACCCAGACGGAACCAAGTCGCTGAAGCTGGGAGACTTTGGGCTGGCGACGGTGGTTGAA GGCCCGTTGTACACAGTCTGTGGCACCCCAACTTACGTGGCACCCGAGATCATTGCTGAAACAGGTTACG GCCTGAAGGTGGATGTTTGGGCAGCTGGTGTGATTACGTACATTCTTCTCTGTGGATTCCCACCGTTCCG GAGTGAGAACAACCTCCAGGAGGATCTCTTTGACCAGATCTTGGCCGGAAAGCTGGAGTTTCCAGCTCCC TACTGGGACAACATTACAGACTCTGCCAAGGAGTTAATCAGTCAAATGCTTCAGGTAAACGTTGAAGCTC GCTGTACTGCGGGAGAAATTCTGAGTCACCCCTGGGTGTCAGATGATGCATCCCAGGAGAATAATATGCA AGCCGAGGTTACAGGTAAACTAAAACAGCACTTTAATAATGCGCTCCCCAAACAGAACAGCACCACCACT GGGGTCTCCGTTATCATGAACACGGCTCTAGATAAAGAGGGGCAGGTTTTCTGCAGCAAGCACTGTCGGG ACAGCAGCAAATCATCCAGGGAGCAGACCTCAGCCCGGGAGGCCCCGCCCCCACCGGAGTCTCCCAGGCC CCCTGGTCCTCCAGCCACCTCTGGCTGTGATCCTGCCGGGACCTGGCGTCGCCATCGAGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR209497 representing NM_001009691
Red=Cloning site Green=Tags(s) MASTRSIELEHFEERDKRPRPGSRRGAPSSSGGSSISGPKGNGLIPSPAHSAHCSFYRTRTLQALSSEKK AKKARFYRNGDRYFKGLVFAISSDRFRSFDALLIELTRSLSDNVNLPQGVRTIYTVDGSRKVTSLDELLE GESYVCASNEPFRKVDYTKNVNPNWSVNIKGGTTRTLAVASAKSEVKESKDFIKPKLVTVIRSGVKPRKA VRILLNKKTAHSFEQVLTDITEAIKLDSGVVKRLCTLDGKQVTCLQDFFGDDDVFIACGPEKYRYAQDDF VLDHSECRVLKSSYSRASAAKYSGSRSPGLSRRSKSPASVKRAGHSSAYSTAKSPVNGTPSSQLSTPKST KSSSSSPTSPGSFRGLKQISAQGRSSSNVNGGPELDRCMSPEGVNGNRCSESFTLLEKYRIGKVIGDGNF AVVKECMDRSTGKEFALKIIDKAKCCGKEHLIENEVSILRRVKHPNIIMLVEEMETTTELFLVMELVKGG DLFDAITSSTKYTERDGSAMVYNLASALRYLHGLSIVHRDIKPENLLVCEYPDGTKSLKLGDFGLATVVE GPLYTVCGTPTYVAPEIIAETGYGLKVDVWAAGVITYILLCGFPPFRSENNLQEDLFDQILAGKLEFPAP YWDNITDSAKELISQMLQVNVEARCTAGEILSHPWVSDDASQENNMQAEVTGKLKQHFNNALPKQNSTTT GVSVIMNTALDKEGQVFCSKHCRDSSKSSREQTSAREAPPPPESPRPPGPPATSGCDPAGTWRRHRD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001009691 |
ORF Size | 2301 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001009691.1, NM_001009691.2, NM_001009691.3, NP_001009691.3 |
RefSeq Size | 3969 bp |
RefSeq ORF | 2304 bp |
Locus ID | 310698 |
Cytogenetics | 2q34 |
MW | 84 kDa |
Gene Summary | This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmoduline-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. Mouse studies show that this gene and the DCX gene, another family member, share function in the establishment of hippocampal organization and that their absence results in a severe epileptic phenotype and lethality, as described in human patients with lissencephaly. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Sep 2010] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN209497 | Dclk2 (untagged ORF) - Rat doublecortin-like kinase 2 (Dclk2), (10 ug) |
USD 1,040.00 |
|
RR209497L3 | Lenti ORF clone of Dclk2 (Myc-DDK-tagged ORF) - Rat doublecortin-like kinase 2 (Dclk2), (10 ug) |
USD 1,230.00 |
|
RR209497L4 | Lenti ORF clone of Dclk2 (mGFP-tagged ORF) - Rat doublecortin-like kinase 2 (Dclk2), (10 ug) |
USD 1,350.00 |
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