Cops3 (NM_001004200) Rat Tagged ORF Clone

CAT#: RR209519

Cops3 (Myc-DDK-tagged ORF) - Rat COP9 constitutive photomorphogenic homolog subunit 3 (Arabidopsis) (Cops3), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cops3
• Synonyms: MGC94156
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209519 representing NM_001004200
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCTGCTCTGGAGCAGTTCGTGAACAGTGTCCGACAGCTCTCAGCTCAAGGGCAGATGACTCAGC
  TTTGTGAACTCATCAACAAGAGTGGAGAACTCCTTGCGAAGAACTTGTCCCACTTGGACACTGTGCTTGG
  GGCTTTAGATGTTCAAGAACACTCATTGGGTGTCCTTGCCGTTCTGTTTGTGAAGTTTTCCATGCCCAGT
  GTTCCTGACTTCGAGACACTGTTCTCACAGGTGCAGCTTTTCATTAGTACTTGTAACGGCGAGCACATTC
  GATACGCAACAGATACGTTCGCTGGGCTTTGCCATCAGCTAACAAACGCACTCGTGGAGAGAAAACAGCC
  CCTTCGAGGAATTGGCATCCTTAAGCAAGCCATAGACAAGATGCAGATGAATACAAACCAGCTGACCTCA
  GTACACGCCGATCTCTGCCAGCTCTGTTTACTAGCGAAGTGCTTTAAACCTGCCCTGCCATATCTTGATG
  TGGATATGATGGATATCTGTAAAGAGAATGGAGCCTATGATGCAAAACACTTTCTGTGCTACTACTATTA
  TGGAGGGATGATCTATACGGGGCTGAAGAACTTTGAAAGAGCACTGTATTTTTATGAGCAGGCCATAACT
  ACCCCAGCCATGGCCGTCAGCCATATCATGTTGGAGTCATATAAAAAGTATATTTTAGTGTCTTTGATAT
  TACTTGGGAAAGTACAACAGCTGCCTAAATACACCTCTCAAATTGTGGGCAGATTTATTAAGCCTCTCAG
  CAACGCTTACCACGAGTTAGCACAAGTTTATTCAACCAACAACCCGTCCGAGCTCCGGAACCTGGTGAAC
  AAGCACAGTGAGACCTTCACTCGAGATAACAACATGGGCCTGGTGAAGCAGTGCCTGTCGTCTCTTTATA
  AGAAGAACATTCAGAGGCTGACAAAGACCTTTTTAACACTTTCATTACAAGATATGGCAAGTCGCGTGCA
  GTTATCTGGACCTCAGGAGGCAGAAAAGTATGTTCTACACATGATAGAAGATGGAGAAATTTTTGCAAGT
  ATTAACCAGAAGGATGGTATGGTCAGTTTCCATGATAATCCTGAAAAGTATAACAACCCAGCCATGCTTC
  ATAACATTGATCAAGAGATGCTAAAATGCATAGAGCTGGATGAGCGACTGAAGGCCATGGACCAGGAGAT
  CACAGTGAACCCCCAGTTTGTTCAAAAGAGCATGGGCTCACAAGAAGATGACTCAGGAAACAAGCCCTCC
  AGCTACTCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209519 representing NM_001004200
  Red=Cloning site Green=Tags(s)
  
  MASALEQFVNSVRQLSAQGQMTQLCELINKSGELLAKNLSHLDTVLGALDVQEHSLGVLAVLFVKFSMPS
  VPDFETLFSQVQLFISTCNGEHIRYATDTFAGLCHQLTNALVERKQPLRGIGILKQAIDKMQMNTNQLTS
  VHADLCQLCLLAKCFKPALPYLDVDMMDICKENGAYDAKHFLCYYYYGGMIYTGLKNFERALYFYEQAIT
  TPAMAVSHIMLESYKKYILVSLILLGKVQQLPKYTSQIVGRFIKPLSNAYHELAQVYSTNNPSELRNLVN
  KHSETFTRDNNMGLVKQCLSSLYKKNIQRLTKTFLTLSLQDMASRVQLSGPQEAEKYVLHMIEDGEIFAS
  INQKDGMVSFHDNPEKYNNPAMLHNIDQEMLKCIELDERLKAMDQEITVNPQFVQKSMGSQEDDSGNKPS
  SYS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001004200
• ORF Size: 1269 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001004200.1, NP_001004200.1
• RefSeq Size: 1596 bp
• RefSeq ORF: 1272 bp
• Locus ID: 287367
• Cytogenetics: 10q22
• MW: 47.9 kDa
• Gene Summary: subunit of the COP9 signalosome complex; mouse homolog plays a role in early embryonic development [RGD, Feb 2006]