Pfkfb3 (NM_057135) Rat Tagged ORF Clone

CAT#: RR209579

Pfkfb3 (Myc-DDK-tagged ORF) - Rat 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (Pfkfb3), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pfkfb3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209579 representing NM_057135
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTTTGGAACTGACCCAGAGCCGGGTGCAGAAGATCTGGGTGCCTGTCGATCACCGACCCTCGTTGC
  CCAGATCCTGTGGGCCGAAGCTGACCAACTCCCCAACGGTGATTGTGATGGTGGGGCTCCCAGCCCGGGG
  TAAGACTTACATCTCCAAGAAGCTGACTCGCTACCTCAACTGGATAGGTGTTCCAACGAAAGTGTTCAAT
  GTGGGAGAATACCGACGGGAGGCTGTGAAGCAATACAGCTCTTACAACTTCTTCCGCCCTGACAATGAGG
  AGGCCATGAGAGTCCGAAAACAGTGTGCCTTAGCTGCCCTGAGAGACGTCAAAAGCTACCTGACAAAGGA
  AGGAGGACAGATTGCAGTTTTTGATGCCACCAATACGACGAGAGAAAGGAGGCACATGATCCTTCATTTT
  GCCAAAGAAAATGACTTCAAGGCATTTTTCATCGAGTCCGTCTGTGACGACCCTACAGTTGTGGCCTCCA
  ATATCATGGAAGTGAAAATCTCCAGCCCGGATTACAAAGACTGCAACTCCGCAGAAGCCATGGACGACTT
  CATGAAGAGGATCAACTGCTACGAAGCCAGCTACCAGCCTCTTGACCCTGATAAATGTGACAGGGACCTG
  TCATTCATCAAAGTGATTGACGTTGGCCGGAGGTTCTTGGTGAACAGAGTGCAGGACCACATCCAGAGCC
  GAATCGTGTATTACCTGATGAACATCCACGTGCAGCCCCGGACCATCTACCTGTGTCGGCACGGCGAGAA
  TGAGTACAACGTGCAGGGCAAGATTGGGGGCGACTCAGGCCTGTCCAGCAGAGGCAAGAAGTTCGCCAAT
  GCCCTGAGCAAGTTCGTTGAAGAGCAGAATCTGAAGGACCTCCGCGTGTGGACCAGTCAGCTGAAAAGTA
  CCATCCAGACCGCGGAGGCCCTAAGGCTCCCCTACGAGCAGTGGAAGGCGCTCAATGAGATTGATGCTGG
  TGTGTGTGAGGAACTAACCTATGAGGAGATCAGAGACACCTACCCTGAGGAGTATGCACTGCGTGAGCAA
  GACAAATACTACTACCGCTATCCCACAGGCGAGTCCTACCAGGATCTTGTCCAGCGCCTGGAGCCTGTGA
  TCATGGAATTAGAGCGCCAAGAGAACGTATTGGTCATCTGTCACCAGGCTGTCCTGCGCTGCCTACTAGC
  CTACTTCCTTGACAAGAGTGCAGAGGAGATGCCATACCTGAAATGCCCACTTCACACAGTCCTGAAACTG
  ACGCCCGTGGCGTATGGGTGCCGTGTGGAGTCCATTTACTTGAATGTAGAATCAGTGAGCACGCATCGGG
  AGAGGTCAGAGGCTGTCAAAATACAACACTTTGCCAGTGTAGTCAGACCTTCCTCTTACACCGAACTTGA
  CTTTCTGTCTGTGGAGTCCGCAAAACAGGATGCAAAGAAGGGACCTAACCCTCTCATGAGACGCAATAGT
  GTCACCCCACTAGCCAGCCCCGAGCCCACCAAAAAGCCTCGCATCAACAGCTTTGAGGAGCATGTGGCTT
  CCACTTCTGCTGCCCTGCCAAGCTGCCTGCCCCCGGAAGTGCCCACGCAGCTGCCCGGACAACCTTTGCT
  AGGGAAAGCCTGTCTACGAACTGTCTGTCACATTTTTTCAAAGTTTTCTCCTTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209579 representing NM_057135
  Red=Cloning site Green=Tags(s)
  
  MPLELTQSRVQKIWVPVDHRPSLPRSCGPKLTNSPTVIVMVGLPARGKTYISKKLTRYLNWIGVPTKVFN
  VGEYRREAVKQYSSYNFFRPDNEEAMRVRKQCALAALRDVKSYLTKEGGQIAVFDATNTTRERRHMILHF
  AKENDFKAFFIESVCDDPTVVASNIMEVKISSPDYKDCNSAEAMDDFMKRINCYEASYQPLDPDKCDRDL
  SFIKVIDVGRRFLVNRVQDHIQSRIVYYLMNIHVQPRTIYLCRHGENEYNVQGKIGGDSGLSSRGKKFAN
  ALSKFVEEQNLKDLRVWTSQLKSTIQTAEALRLPYEQWKALNEIDAGVCEELTYEEIRDTYPEEYALREQ
  DKYYYRYPTGESYQDLVQRLEPVIMELERQENVLVICHQAVLRCLLAYFLDKSAEEMPYLKCPLHTVLKL
  TPVAYGCRVESIYLNVESVSTHRERSEAVKIQHFASVVRPSSYTELDFLSVESAKQDAKKGPNPLMRRNS
  VTPLASPEPTKKPRINSFEEHVASTSAALPSCLPPEVPTQLPGQPLLGKACLRTVCHIFSKFSPY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_057135
• ORF Size: 1665 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_057135.1, NP_476476.1
• RefSeq Size: 2148
• RefSeq ORF: 1668
• Locus ID: 117276
• MW: 63.7 kDa
• Gene Summary: catalyzes the biosynthesis and degradation of fructose 2,6-bisphosphate, which plays a role in glycolysis [RGD, Feb 2006]