Mapk1 (NM_053842) Rat Tagged ORF Clone

CAT#: RR209609

Mapk1 (Myc-DDK-tagged ORF) - Rat mitogen activated protein kinase 1 (Mapk1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mapk1
• Synonyms: ERK-2; Erk2; ERT1; p42-MAPK
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209609 representing NM_053842
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGCGGCGGCGGCGGGCCCGGAGATGGTCCGCGGGCAGGTGTTCGACGTGGGGCCGCGCTACA
  CTAATCTCTCGTACATCGGAGAAGGCGCCTACGGCATGGTTTGTTCTGCTTATGATAATCTCAACAAAGT
  TCGAGTTGCTATCAAGAAAATCAGTCCTTTTGAGCACCAGACCTACTGTCAGAGAACCCTGAGAGAGATA
  AAAATCCTACTGCGCTTCAGACATGAGAACATCATCGGCATCAATGACATCATCCGGGCACCAACCATTG
  AGCAGATGAAAGATGTATATATAGTACAGGACCTCATGGAGACAGATCTTTACAAGCTCTTGAAGACACA
  GCACCTCAGCAATGATCATATCTGCTATTTTCTTTATCAGATCCTGAGAGGATTAAAGTATATACATTCA
  GCTAATGTTCTGCACCGTGACCTCAAGCCTTCCAACCTCCTGCTGAACACCACTTGTGATCTCAAGATCT
  GTGACTTTGGCCTTGCCCGTGTTGCAGATCCAGACCATGATCATACAGGGTTCTTGACAGAGTATGTAGC
  CACGCGTTGGTACAGAGCTCCAGAAATTATGTTGAATTCCAAGGGTTATACCAAGTCCATTGATATTTGG
  TCTGTGGGCTGCATCCTGGCAGAGATGCTATCCAACAGGCCTATCTTCCCAGGAAAGCATTACCTTGACC
  AGCTGAATCACATCCTGGGTATTCTTGGATCTCCATCACAGGAAGATCTGAATTGTATAATAAATTTAAA
  AGCTAGAAACTATTTGCTTTCTCTCCCGCACAAAAATAAGGTGCCGTGGAACAGGTTGTTCCCAAACGCT
  GACTCCAAAGCTCTGGATTTACTGGATAAAATGTTGACATTTAACCCTCACAAGAGGATTGAAGTTGAAC
  AGGCTCTGGCCCACCCGTACCTGGAGCAGTATTATGACCCAAGTGATGAGCCCATTGCTGAAGCACCATT
  CAAGTTTGACATGGAGCTGGACGACTTACCTAAGGAGAAGCTCAAAGAACTCATTTTTGAAGAGACTGCT
  CGATTCCAGCCAGGATACAGATCT
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209609 representing NM_053842
  Red=Cloning site Green=Tags(s)
  
  MAAAAAAGPEMVRGQVFDVGPRYTNLSYIGEGAYGMVCSAYDNLNKVRVAIKKISPFEHQTYCQRTLREI
  KILLRFRHENIIGINDIIRAPTIEQMKDVYIVQDLMETDLYKLLKTQHLSNDHICYFLYQILRGLKYIHS
  ANVLHRDLKPSNLLLNTTCDLKICDFGLARVADPDHDHTGFLTEYVATRWYRAPEIMLNSKGYTKSIDIW
  SVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLNCIINLKARNYLLSLPHKNKVPWNRLFPNA
  DSKALDLLDKMLTFNPHKRIEVEQALAHPYLEQYYDPSDEPIAEAPFKFDMELDDLPKEKLKELIFEETA
  RFQPGYRS
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053842
• ORF Size: 1074 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_053842.1, NM_053842.2, NP_446294.1
• RefSeq Size: 5615
• RefSeq ORF: 1077
• Locus ID: 116590
• MW: 41.3 kDa
• Gene Summary: kinase involved in intracellular signaling; component of Mapk signaling pathway [RGD, Feb 2006]