Pigm (NM_024144) Rat Tagged ORF Clone

CAT#: RR209711

Pigm (Myc-DDK-tagged ORF) - Rat phosphatidylinositol glycan anchor biosynthesis, class M (Pigm), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pigm
• Synonyms: GPI-MT-I
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209711 representing NM_024144
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTACACCAAGCACTGGGGAGAATGGTTCCTGAACCTGCGGGTGCCGCCCGCCGGGGTGTTCGGCG
  TGGCCTTCCTGGCCCGGGTCGCCCTGGTTTTCTACGGTGTCTTTCAGGACCGGACTCTGCTCGTGAGGTA
  CACCGACATAGACTACCACGTGTTCACGGACGCCGCGCGTTTCGTCACGGAGGGGCGCTCCCCTTACCTG
  AGGGCGACGTATCGCTACACGCCGCTCCTGAGTTGGCTCCTCACTCCCAACGTCTACCTCAGCGAACTCT
  TTGGCAAGTTTCTCTTCATCAGCTGCGACCTCCTCACCGCCTTCCTCTTGTACCGCCTGCTGCTGCTCAA
  GGGGCTGGGGCGCCGCCAGGCCTGTGGCTACTGCGTCTTCTGGCTACTTAACCCCTTACCCATGGCGGTG
  TCCAGCCGAGGCAACGCAGACTCCATCGTCGCCTCCCTGGTGCTTACGACCCTGTACTTAATAGAGAAAC
  GACTCATCGCGTGTGCCGCGGTGTTCTATGGCTTCGCGGTGCACATGAAGATGTATCCGGTGACCTACAT
  CCTCCCCATAGCGCTCCATTTGCGTCCCGAGCGCGACAGTGACGAGGGGCTCCGGCTAGCCCGGTACTCT
  TTCCAGGCTCGTTTGTACGACTTCCTGAAGAGGCTGTGTAGCTGGGCTGTGCTGCTTTTCGTAGCAATTG
  CAGGACTCACTTTTTTGGCCCTGAGCTTCGGTTTTTACTATAAGTACGGTTGGGAGTTTCTGGAGCACAC
  CTACCTTTATCACCTGACTCGGAGGGATATCCGCCATAACTTTTCTCCATATTTCTATATGCTGTATCTG
  ACTGCAGAGAGCAAGTGGAGTTTCACGCTGGGGATCGCTGCGTTCCTGCCACAGTTCATCTTGCTTTCAG
  CCGCGTCTTTTGCCTATTACAGAGACCTTGTCTTCTGTTGTTTTCTTCATACATCCATTTTTGTGACTTT
  TAACAAAGTCTGCACTTCTCAGTACTTTCTTTGGTACCTCTGCCTTCTGCCTCTGGTGATGCCACTAGTG
  AGGATGCCTTGGAAGAGAGCCGTGGTCCTCTTAATGTTGTGGTTCATAGGACAGGCCTTATGGCTGGCTC
  CTGCATACGTTCTAGAGTTTCAAGGAAAGAACACCTTCCTGTTTATTTGGTTAGCTGGTTTGTTCTTCCT
  TCTGATCAACTGTTCTATACTGATTCAGATTATTTCCCATTACAAGGAGGACCGCCTAACCGAGAGAATC
  AAATACGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209711 representing NM_024144
  Red=Cloning site Green=Tags(s)
  
  MSYTKHWGEWFLNLRVPPAGVFGVAFLARVALVFYGVFQDRTLLVRYTDIDYHVFTDAARFVTEGRSPYL
  RATYRYTPLLSWLLTPNVYLSELFGKFLFISCDLLTAFLLYRLLLLKGLGRRQACGYCVFWLLNPLPMAV
  SSRGNADSIVASLVLTTLYLIEKRLIACAAVFYGFAVHMKMYPVTYILPIALHLRPERDSDEGLRLARYS
  FQARLYDFLKRLCSWAVLLFVAIAGLTFLALSFGFYYKYGWEFLEHTYLYHLTRRDIRHNFSPYFYMLYL
  TAESKWSFTLGIAAFLPQFILLSAASFAYYRDLVFCCFLHTSIFVTFNKVCTSQYFLWYLCLLPLVMPLV
  RMPWKRAVVLLMLWFIGQALWLAPAYVLEFQGKNTFLFIWLAGLFFLLINCSILIQIISHYKEDRLTERI
  KYD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024144
• ORF Size: 1269 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_024144.1, NP_077058.1
• RefSeq Size: 3700
• RefSeq ORF: 1272
• Locus ID: 79112
• MW: 49.6 kDa
• Gene Summary: mannosyltransferase that catalyzes the transfer of the first mannose to glycosylphosphatidylinositol in GPI anchor biosynthesis; activity may be localized to the lumenal side of the endoplasmic reticulum membrane [RGD, Feb 2006]