Efemp2 (NM_001005907) Rat Tagged ORF Clone

CAT#: RR209743

Efemp2 (Myc-DDK-tagged ORF) - Rat EGF-containing fibulin-like extracellular matrix protein 2 (Efemp2), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Efemp2
• Synonyms: MGC94840
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209743 representing NM_001005907
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCCCATTTGCCTCCTGCCTCCCCGGGTCTTTACTGCTCTGGGCGTTTCTGCTGTTGCTCTTGGGAG
  CAGCGTCCCCACAGGATTCCGAAGAGCCGGACAGCTACACGGAATGCACAGATGGCTATGAGTGGGATGC
  AGACAGCCAGCATTGCCGGGATGTCAATGAGTGCCTGACCATCCCCGAGGCTTGCAAGGGTGAAATGAAA
  TGCATCAACCACTACGGGGGCTATTTGTGTCTGCCTCGCTCTGCTGCCGTCATCAGTGATCTCCACAGTG
  AAGGACCTCCACCACCAGCAGCCTCTGCTCAACACCCAAATCCTTGCCCTCCGGGCTATGAACCTGATGA
  ACAGGAGAGCTGTGTGGACATAGATGAGTGTCGCTACCGCTACTGCCAGCACCGATGCGTGAACCTGCCA
  GGCTCTTTCCGATGCCAGTGTGAGCCCGGCTTCCAGTTGGGACCTAACAACCGCTCTTGTGTGGATGTGA
  ACGAGTGTGACATGGGAGCCCCGTGTGAGCAGCGCTGCTTCAACTCCTATGGGACCTTCCTGTGTCGCTG
  TAACCAGGGCTATGAGCTACACCGGGATGGCTTCTCCTGCAGTGATATCGATGAGTGCAGCTACTCCAGT
  TACCTCTGCCAGTACCGCTGTGTCAATGAGCCAGGTCGCTTCTCCTGTCACTGCCCACAAGGCTACCAAC
  TGCTGGCCACAAGGCTCTGCCAAGACATTGACGAGTGCGAAACAGGTGCACACCAGTGTTCGGAGGCCCA
  AACCTGCGTCAACTTCCATGGGGGTTACCGCTGTGTGGACACCAACCGTTGTGTGGAGCCCTATGTCCAA
  GTGTCGGACAACCGCTGCCTCTGTCCTGCCTCCAACCCCCTTTGTCGAGAGCAACCGTCATCCATCGTGC
  ACCGCTACATGAGCATCACCTCAGAGCGAAGTGTGCCTGCCGACGTGTTTCAGATCCAGGCAACCTCTGT
  CTACCCTGGCGCCTACAATGCCTTTCAGATCCGTGCTGGGAACACACAGGGGGACTTCTACATTAGGCAA
  ATCAACAATGTCAGCGCCATGCTGGTCCTTGCCAGACCGGTGACAGGACCCCGGGAGTACGTCCTGGACC
  TCGAGATGGTCACCATGAACTCCCTTATGAGTTACCGGGCCAGCTCTGTACTGAGACTCACCGTCTTTGT
  GGGAGCCTATACCTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209743 representing NM_001005907
  Red=Cloning site Green=Tags(s)
  
  MLPFASCLPGSLLLWAFLLLLLGAASPQDSEEPDSYTECTDGYEWDADSQHCRDVNECLTIPEACKGEMK
  CINHYGGYLCLPRSAAVISDLHSEGPPPPAASAQHPNPCPPGYEPDEQESCVDIDECRYRYCQHRCVNLP
  GSFRCQCEPGFQLGPNNRSCVDVNECDMGAPCEQRCFNSYGTFLCRCNQGYELHRDGFSCSDIDECSYSS
  YLCQYRCVNEPGRFSCHCPQGYQLLATRLCQDIDECETGAHQCSEAQTCVNFHGGYRCVDTNRCVEPYVQ
  VSDNRCLCPASNPLCREQPSSIVHRYMSITSERSVPADVFQIQATSVYPGAYNAFQIRAGNTQGDFYIRQ
  INNVSAMLVLARPVTGPREYVLDLEMVTMNSLMSYRASSVLRLTVFVGAYTF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001005907
• ORF Size: 1206 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001005907.1, NM_001005907.2, NP_001005907.1
• RefSeq Size: 1450 bp
• RefSeq ORF: 1209 bp
• Locus ID: 293677
• Cytogenetics: 1q43
• MW: 44.9 kDa