Eif2b5 (NM_138866) Rat Tagged ORF Clone

CAT#: RR209837

Eif2b5 (Myc-DDK-tagged ORF) - Rat eukaryotic translation initiation factor 2B, subunit 5 epsilon (Eif2b5), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Eif2b5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209837 representing NM_138866
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCCACTGCGGCGGTTCCCAGCGCTGTAGGCGGCCGAGCGAACAAACGCGGCGGCGGCTCCGGCG
  GCGGTGGAACCCAAGGTGCGGAGGAGGAGCCGCCGCCGCCCCTCCAGGCAGTTTTAGTGGCCGATAGCTT
  CGACCGCCGCTTCTTCCCCATCTCCAAGGACCAGCCTCGGGTCCTATTGCCCCTGGCTAATGTCGCGCTG
  ATCGACTATACTCTGGAATTCTTGACTGCCACGGGTGTACAGGAAACATTTGTCTTTTGCTGCTGGAAGG
  CTGCTCAGATCAAAGAACACTTACAGAAATCCAAATGGTGCCATCCTACGTCCCTCAATGTTGTTCGAAT
  AATCACATCAGACCTGTACCGATCACTAGGAGATGTCCTCCGTGATGTCGATGCCAAGGCCTTGGTGCGC
  TCTGATTTCCTCCTGATATATGGAGATGTCGTCTCAAATATCAATATTTCCAAAGCCCTGGAGGAACACA
  GGTTAAGAAGGAAGCTAGAAAAAAATGTGTCTGTCATGACAATGGTGTTCAAAGAGTCCTCACCCAGTCA
  CCCTACACGCTGCCATGAGGACAATGTGGTGCTAGCTGTGGATAGCACCACAAACAGGATTCTTCACTTC
  CAGAAGACCCAAGGCCTCCGCCATTTCTCCTTTCCTTTGGGCCTATTCCAGGGCAGTCTAGACGGAGTGG
  AGATTCGATATGACTTGTTGGATTGTCACATCAGCATCTGCTCTCCTCAGGTAGCTCAGCTCTTCACAGA
  CAACTTTGACTACCAAACTCGAGATGATTTTGTGCGAGGCCTGCTAGTGAATGAGGAGATCCTAGGAAAT
  CAGATTCACTTGCATGTGACAAGTAGGGAATATGGTGCCCGGGTCTCCAACCTACACATGTACTCAGCTG
  TCTGTGCTGACGTCATCCGCCGATGGGTCTACCCTCTTACTCCAGAAGTCAACTTCACTGACAGCAGTAC
  ACAAAGCTACACTCATTCCCGACACAACATCTACCGAGGGCCTGAAGTCAGCCTGGGCCACGGCAGCGTC
  CTGGAGGAAAACGTGCTTCTGGGAGCTGGCACTGTCGTTGGCAGCAACTGCTCCATCACCAACAGTGTCA
  TTGGCCCTAACTGCCACATTGGTGATAATGTGGTACTGGACCAGGCCTACCTGTGGCAGGGAGTTCGAGT
  GGCTGCTGGAGCACAAATACACCAGTCTCTGCTCTGTGACAGAGCTGAGGTCAAGGAGCGAGTCATACTG
  AAGCCACACTGTGTCCTCACTTCCCAGGTGGTAGTGGGCCCAGACATCATACTGCCCGAGGGCTCGGTGA
  TCTCTTTGCACCCTCCAGATGCAGAGGAAGATGAAGATGATGGCCAGTTTAGTGATGATTCTGGCGCTGA
  CCAAGAAAAGGAGAAAGTGAAGCTGAAAGGTTACAATCCGGCAGAAGTTGGCCCTGAAGGCCAGGGATAC
  CTCTGGAAAGCTGAGGATGTGGATGAGAAGGAAGATGAAGAGCTGCGGCAGAGTCTCTGGGGACTCATGA
  TCAATATGGAGGAGGAAAGTGAGACGGAAAGTGAGCGGAGCGTGGATCCCGAGGAGCTGGACAGCCGAGC
  AGGCTCCCCTCAGCTGGATGACATCAGAGTTTTCCAGAACGAGGTCTTAGGAACGCTGCAGCGGGGCAGA
  GAGGAGAACATCTCCTGTGACAATCTAGTCCTGGAGATCAACTCCCTCAAGTATGCCTACAACATTAGCC
  TAAAGGAAGTGATGCAGGTCCTGAGCCATGTGGTCCTGGAGTTCCCCCTGCAACAAGTAGATGGCGTGCT
  TGACCCAAACCGCTACTGTGCCCTGCTGCTTCCTCTGCTCAAAGCCTGGAGCCCTGTTTTTCGGAACTAC
  ATAAAGCGCGCAGCTGACCATTTGGAAGCATTGGCGGCCATTGAGGACTTCTTCTTGGAACATGAAACTC
  TTGTTCCTTCCCTGGCCAAGGTCCTGATGGCTTTCTACCAGCTGGAGATCTTGGCTGAGGAAACAATCTT
  GAGCTGGTTCAGCCAAAGAGACATAACTGACAAAGGCCAGCAGTTACGTAAGAATCAACAGCTGCAGAGG
  TTCATCCAGTGGCTGAGAGAGGCAGAAGAGGAGTCATCTGACGATGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209837 representing NM_138866
  Red=Cloning site Green=Tags(s)
  
  MAATAAVPSAVGGRANKRGGGSGGGGTQGAEEEPPPPLQAVLVADSFDRRFFPISKDQPRVLLPLANVAL
  IDYTLEFLTATGVQETFVFCCWKAAQIKEHLQKSKWCHPTSLNVVRIITSDLYRSLGDVLRDVDAKALVR
  SDFLLIYGDVVSNINISKALEEHRLRRKLEKNVSVMTMVFKESSPSHPTRCHEDNVVLAVDSTTNRILHF
  QKTQGLRHFSFPLGLFQGSLDGVEIRYDLLDCHISICSPQVAQLFTDNFDYQTRDDFVRGLLVNEEILGN
  QIHLHVTSREYGARVSNLHMYSAVCADVIRRWVYPLTPEVNFTDSSTQSYTHSRHNIYRGPEVSLGHGSV
  LEENVLLGAGTVVGSNCSITNSVIGPNCHIGDNVVLDQAYLWQGVRVAAGAQIHQSLLCDRAEVKERVIL
  KPHCVLTSQVVVGPDIILPEGSVISLHPPDAEEDEDDGQFSDDSGADQEKEKVKLKGYNPAEVGPEGQGY
  LWKAEDVDEKEDEELRQSLWGLMINMEEESETESERSVDPEELDSRAGSPQLDDIRVFQNEVLGTLQRGR
  EENISCDNLVLEINSLKYAYNISLKEVMQVLSHVVLEFPLQQVDGVLDPNRYCALLLPLLKAWSPVFRNY
  IKRAADHLEALAAIEDFFLEHETLVPSLAKVLMAFYQLEILAEETILSWFSQRDITDKGQQLRKNQQLQR
  FIQWLREAEEESSDDD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_138866
• ORF Size: 2148 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_138866.1, NM_138866.2, NP_620221.2
• RefSeq Size: 3147
• RefSeq ORF: 2151
• Locus ID: 192234
• MW: 80.2 kDa
• Gene Summary: subunit of eukaryotic initiation factor-2B, which plays a role in eukayrotic initiation factor 2 (eIF-2) recycling in translation initiation [RGD, Feb 2006]