Ugcg (NM_031795) Rat Tagged ORF Clone

CAT#: RR209871

Ugcg (Myc-DDK-tagged ORF) - Rat UDP-glucose ceramide glucosyltransferase (Ugcg), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ugcg
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209871 representing NM_031795
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCTGCTGGACCTGGCCCAGGAGGGAATGGCCTTGTTCGGCTTCGTGCTCTTCGTGGTGCTGTGGC
  TGATGCATTTCATGTCCATCATCTACACCCGGTTACACCTCAACAAGAAGGCAACAGACAAACAGCCGTA
  CAGCAAGCTCCCTGGAGTCTCTCTTCTGAAACCACTGAAGGGGGTGGACCCTAACCTAATCAACAACTTG
  GAGACATTCTTTGAACTGGATTATCCCAAATATGAAGTACTCCTTTGTGTACAAGATCATGATGATCCAG
  CCATTGAGGTGTGTAAGAAACTGCTTGGAAAATACCCAAACGTTGATGCTAGATTATTTATAGGTGGTAA
  AAAGGTTGGCATTAATCCTAAAATTAATAATTTGATGCCAGCATATGAAGTTGCAAAGTATGATCTCATA
  TGGATTTGTGATAGCGGAATACGAGTCATTCCAGACACATTAACTGACATGGTCAATCAGATGACGGAGA
  GAGTGGGGCTGGTCCACGGGCTTCCATACGTAGCTGACAGACAAGGCTTTGCTGCCACCTTAGAGCAGGT
  ATATTTTGGAACTTCACACCCAAGATCCTATATCTCTGCCAATGTGACCGGCTTCAAGTGTGTGACGGGG
  ATGTCTTGCTTGATGAGGAAGGATGTGCTAGATCAGGCAGGAGGGCTCATAGCCTTTGCTCAGTACATTG
  CGGAAGATTACTTCATGGCCAAAGCAATAGCGGACCGAGGCTGGAAGTTTTCAATGTCTACGCAAGTTGC
  CATGCAAAACTCTGGTTCATACTCCATTTCTCAGTTTCAATCCAGAATGATCAGGTGGACCAAATTGAGG
  ATTAACATGCTTCCTGCCACAATAATTTGTGAGCCAATTTCAGAATGCTTTGTTGCCAGTTTAATTATCG
  GGTGGGCAGCCCACCACGTGTTCCGATGGGATATCATGGTTTTCTTCATGTGTCACTGCCTGGCATGGTT
  TATATTTGACTACATTCAACTCAGGGGTGTCCAGGGTGGCACACTATGCTTTTCAAAACTTGATTATGCT
  GTGGCTTGGTTCATCCGCGAATCCATGACAATATACATTTTCTTGTCGGCACTGTGGGACCCTACTATAA
  GCTGGAGAGCCGGTCGCTACAGGTTGCGCTGTGGAGGGACGGCCGAGGAGATCCTGGATGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209871 representing NM_031795
  Red=Cloning site Green=Tags(s)
  
  MALLDLAQEGMALFGFVLFVVLWLMHFMSIIYTRLHLNKKATDKQPYSKLPGVSLLKPLKGVDPNLINNL
  ETFFELDYPKYEVLLCVQDHDDPAIEVCKKLLGKYPNVDARLFIGGKKVGINPKINNLMPAYEVAKYDLI
  WICDSGIRVIPDTLTDMVNQMTERVGLVHGLPYVADRQGFAATLEQVYFGTSHPRSYISANVTGFKCVTG
  MSCLMRKDVLDQAGGLIAFAQYIAEDYFMAKAIADRGWKFSMSTQVAMQNSGSYSISQFQSRMIRWTKLR
  INMLPATIICEPISECFVASLIIGWAAHHVFRWDIMVFFMCHCLAWFIFDYIQLRGVQGGTLCFSKLDYA
  VAWFIRESMTIYIFLSALWDPTISWRAGRYRLRCGGTAEEILDV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031795
• ORF Size: 1182 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_031795.1, NM_031795.2, NP_113983.1
• RefSeq Size: 3746
• RefSeq ORF: 1185
• Locus ID: 83626
• MW: 44.8 kDa
• Gene Summary: catalyzes the transfer of glucose from UDP-glucose (UDP-Glc) to ceramide to form glucosylceramide [RGD, Feb 2006]