Sphk1 (NM_133386) Rat Tagged ORF Clone

CAT#: RR209901

Sphk1 (Myc-DDK-tagged ORF) - Rat sphingosine kinase 1 (Sphk1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Sphk1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209901 representing NM_133386
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAACCAGCAGACTGTCCCCGGGGACTGCTCCCACGGCCATGCAGAGTGTTGGTGCTGCTGAACCCCC
  GGGGTGGCAAGGGCAAGGCTCTGAAGCTCTTTCAGAGCCGTGTGCGGCCCCTTCTGGAGGAGGCTGAGGT
  ATCCTTTAAACTGATGCTCACCGAACGGCAGAACCATGCCAGGGAGCTGGTGTGTGCAGAAGAACTGGGT
  CACTGGGATGCCTTGGCGGTCATGTCCGGTGACGGTCTGATGCACGAGGTGGTGAATGGGCTAATGGAAC
  GACCTGACTGGGAGAGTGCCATCCAGAAACCCCTATGTAGCCTCCCTGGAGGCTCTGGCAATGCGCTGGC
  AGCTTCTTTGAACTACTATGCTGGGCACGAGCAGGTCACTAATGAAGATCTCCTGATCAACTGCACACTG
  CTGTTATGCTGCCGGCAGCTGTCACCCATGAATCTGCTGTCCCTGCACACCGCTTCCGGACGGCAACTCT
  ATTCTGTTCTCAGTCTGTCCTGGGGTTTCGTTGCTGACGTGGACTTGGAGAGTGAGAAATACAGGAGCTT
  GGGGGAGATTCGTTTCACGGTGGGAACCTTTTTTCGCCTAGCAAGCCTGCGCATTTACCAAGGCCAACTG
  GCCTACCTTCCTGTAGGGAAGGCTGCCTCTAAGATACCTGCTTCCTCACTGGCACAGAAGGGCCCTGCCA
  ACACATACCTCGTTCCTCTGGAGGAGCCAGTGCCACCTCATTGGACTGTGGTGCCAGAGCAGGACTTCGT
  TCTGGTGCTGGTGCTGCTTCACACCCACCTGAGCACAGAGATGTTTGCAGCACCCATGGGCCGCTGCGAG
  GCTGGTGTTATGCATCTGTTCTATATACGTGCGGGGGTGTCAAGGGCCATGCTGCTGCGCCTCTTCCTGG
  CCATGCAGAAGGGCAAGCATATGGACCTCGACTGCCCGTACTTGGTTCATGTGCCAGTGGTTGCCTTCCG
  CCTGGAACCCAGGAACCAGAGGGGAGTGTTTTCTGTGGACGGGGAGCTGATGGTGTGTGAAGCTGTGCAG
  GGCCAAGTGCACCCAAACTACCTTTGGATGGTCTCTGGTAGCAGTGACTCCCCGTCCGGCCGGGACTCCC
  AGCGGAGGCCACCTCCAGAAGAACCAATA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209901 representing NM_133386
  Red=Cloning site Green=Tags(s)
  
  MQPADCPRGLLPRPCRVLVLLNPRGGKGKALKLFQSRVRPLLEEAEVSFKLMLTERQNHARELVCAEELG
  HWDALAVMSGDGLMHEVVNGLMERPDWESAIQKPLCSLPGGSGNALAASLNYYAGHEQVTNEDLLINCTL
  LLCCRQLSPMNLLSLHTASGRQLYSVLSLSWGFVADVDLESEKYRSLGEIRFTVGTFFRLASLRIYQGQL
  AYLPVGKAASKIPASSLAQKGPANTYLVPLEEPVPPHWTVVPEQDFVLVLVLLHTHLSTEMFAAPMGRCE
  AGVMHLFYIRAGVSRAMLLRLFLAMQKGKHMDLDCPYLVHVPVVAFRLEPRNQRGVFSVDGELMVCEAVQ
  GQVHPNYLWMVSGSSDSPSGRDSQRRPPPEEPI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133386
• ORF Size: 1149 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_133386.1, NM_133386.2, NM_133386.3, NP_596877.2
• RefSeq Size: 3134
• RefSeq ORF: 1152
• Locus ID: 170897
• MW: 42.9 kDa
• Gene Summary: catalyzes the conversion of sphingosine to sphingosine 1 phosphate, which plays a role in cell proliferation and differentiation [RGD, Feb 2006]