Sema4a (NM_001012078) Rat Tagged ORF Clone

CAT#: RR209946

Sema4a (Myc-DDK-tagged ORF) - Rat sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A (Sema4a), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Sema4a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209946 representing NM_001012078
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCATGGCCCTACCATCCCTGGGCCAAGACCCCTGGAGTCTCCTGAGTGTTTTTTTCTTCCAACTGT
  TCCTGCTGCTGTCACTGCCACCAGCTTCCGGGACTGGTGGGCAGGGACCCGTGCCCAGAGTCAAGTACCA
  TGCGGGAGATGGGCACAGGGCCCTCAGCTTCTTTCAACAAAAAGGCCTCCGGGACTTTGACACCCTGCTC
  CTGAGTGGAGATGGCAACACTCTGTATGTGGGGGCTCGGGAGGCCATCTTGGCCCTGAATATCGAGAATC
  CAGGAATCCCAAGGCTGAGGAACATGATACCCTGGCCAGCCAGTGAGAAAAAAAAGAACGAATGTGCCTT
  TAAGAAGAAAAGCAATGAGACACAGTGTTTCAACTTCATTCGTGTGCTGGTCTCTTACAATGCCACTCAC
  CTCTATGCCTGCGGGACTTTTGCCTTCAGCCCCGCCTGCACCTTCATTGAACTCCAAGATTCCTTCCTGT
  TGCCCATCTTGAAGGACAAGGTCACAGATGGGAAGGGTCAAAGCCCCTTTGACCCTGCTCACAAGCACAC
  AGCTGTCTTGGTCGATGGGATGCTTTATTCTGGCACCATGAACAACTTCCTGGGCAGCGAACCCATCCTG
  ATGCGGACACTGGGATCCCAGCCTGTTCTCAAGACTGACATCTTCCTACGCTGGCTGCACCCGGACGCCT
  CCTTCGTGGCAGCCATCCCATCTACCCAGGTCGTCTATTTCTTCTTTGAGGAGACAGCCAGCGAGTTTGA
  CTTCTTTGAGGAGCTGTACACATCCAGGGTGGCTCAAGTCTGCAAGAACGACGTAGGCGGTGAAAAGCTG
  CTGCAGAAGAAGTGGACCACCTTCCTGAAAGCCCAGCTGCTCTGCGCTCAGCCAGGGCAGCTGCCATTCA
  ACATCATCCGCCACGCGGTCCTGCTGCCCGCCGATTCTCCCTCTGTTCCCCGCATCTACGCAGTCTTTAC
  CTCCCAGTGGCAGGTTGGTGGGACCAGGAGCTCAGCAGTCTGTGCCTTCTCTCTCACGGACATTGAGCGA
  GTCTTTAAAGGAAAGTACAAGGAGCTGAACAAAGAAACCTCCCGCTGGACCACTTACAGGGGCTCAGAGG
  TCAACCCGAGGCCAGGCAGCTGCTCCATGGGCCCCTCCTCTGACAAAGCCTTGACCTTCATGAAGGACCA
  TTTTCTGATGGATGAGCATGTGGTAGGAGCTCCCCTGCTGGTGAAGTCTGGCGTGGAGTACACACAGCTT
  GCCGTGGAAACAGCTCAGGGCCTTGACGGGAGCAGCCATGTGGTCCTTTACCTGGGTACCTCTACGGGGT
  CCCTGCACAAAGCTGTGGTGCCTCAGAATAGCAGTGCTTATCTTGTGGAGGAGATTCAGCTGAGCCCTAA
  CTCTGAGCCTGTTCAGAACCTGCAGCTGGCCCCCACCCAGGGTGCAGTGTTCGCAGGCTTCTCTGAAGGC
  ATCTGGAGGGTTCCCAGGGCCAACTGCAGTGTCTATAAGAGCTGTGTGGACTGTGTCCTTGCCAGGGACC
  CTCACTGTGCCTGGGACCCTGAATCAAGACTGTGCAGCCTTCTGTCTGGCTCTCCCCTGACTTGGAAGCA
  GGACATGGAACGCGGCAACCCGGAGTGGGCATGCGCCCGTGGCCCCATGGCCAGGAGCCCCCGGCGTCAG
  AGCTCCCCTCAACTCATTAAAGAAGTCCTGACAGTCCCCAACTCCATCCTGGAGCTGCCCTGCCCCCAGC
  TGTCAGCACTGGCCTCTTACCACTGGAGTCACGGCAAAGCCAAAATCCCAGAAGCCTCTTCTACTGTCTA
  TAATGGCTCCCTCTTGTTGCTGCCACAGGATGGCGTTGGGGGCCTCTACCAGTGCGTGGCCACTGAAAAC
  GGCTATTCGTACCCTGTAGTCTCCTACTGGGTAGACAGCCAGGACCAGCCCCTGGCACTGGATCCTGAGC
  TGGCGGGCGTTCCCCGGGAGCGAGTGCAGGTCCCACTGACCAGGGTCGGAGGCGGGGCTTCTATGGCTGC
  CCAGCGGTCCTACTGGCCCCATTTTCTCATCGTTACTGTCCTCCTGGCCATTGTGCTCTTGGGAGTGCTC
  ACTCTCCTCCTTGCTTCCCCACTGGGAGTGCTTCGGGCTCGGGGTAAGGTTCAGGGCTGTGGGATGCTGC
  CCCCCAGGGAAAAGGCCCCACTGAGCAGGGAACAGCACCTCCAGCCTTCCAAGGACCACAGGACCTCTGC
  CAGCGACGTAGATGCTGACAACAACCATCTGGGCGCTGAAGTGGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209946 representing NM_001012078
  Red=Cloning site Green=Tags(s)
  
  MTMALPSLGQDPWSLLSVFFFQLFLLLSLPPASGTGGQGPVPRVKYHAGDGHRALSFFQQKGLRDFDTLL
  LSGDGNTLYVGAREAILALNIENPGIPRLRNMIPWPASEKKKNECAFKKKSNETQCFNFIRVLVSYNATH
  LYACGTFAFSPACTFIELQDSFLLPILKDKVTDGKGQSPFDPAHKHTAVLVDGMLYSGTMNNFLGSEPIL
  MRTLGSQPVLKTDIFLRWLHPDASFVAAIPSTQVVYFFFEETASEFDFFEELYTSRVAQVCKNDVGGEKL
  LQKKWTTFLKAQLLCAQPGQLPFNIIRHAVLLPADSPSVPRIYAVFTSQWQVGGTRSSAVCAFSLTDIER
  VFKGKYKELNKETSRWTTYRGSEVNPRPGSCSMGPSSDKALTFMKDHFLMDEHVVGAPLLVKSGVEYTQL
  AVETAQGLDGSSHVVLYLGTSTGSLHKAVVPQNSSAYLVEEIQLSPNSEPVQNLQLAPTQGAVFAGFSEG
  IWRVPRANCSVYKSCVDCVLARDPHCAWDPESRLCSLLSGSPLTWKQDMERGNPEWACARGPMARSPRRQ
  SSPQLIKEVLTVPNSILELPCPQLSALASYHWSHGKAKIPEASSTVYNGSLLLLPQDGVGGLYQCVATEN
  GYSYPVVSYWVDSQDQPLALDPELAGVPRERVQVPLTRVGGGASMAAQRSYWPHFLIVTVLLAIVLLGVL
  TLLLASPLGVLRARGKVQGCGMLPPREKAPLSREQHLQPSKDHRTSASDVDADNNHLGAEVA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001012078
• ORF Size: 2286 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012078.2, NP_001012078.2
• RefSeq Size: 3179 bp
• RefSeq ORF: 2289 bp
• Locus ID: 310630
• Cytogenetics: 2q34
• MW: 83.5 kDa