Atp6v0d1 (NM_001011927) Rat Tagged ORF Clone

CAT#: RR209969

Atp6v0d1 (Myc-DDK-tagged ORF) - Rat ATPase, H+ transporting, lysosomal 38kDa, V0 subunit d1 (Atp6v0d1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Atp6v0d1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR209969 representing NM_001011927
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGTTCTTCCCGGAGCTTTATTTCAACGTGGACAATGGCTACTTGGAGGGATTAGTGCGCGGCCTGA
  AGGCCGGGGTGCTCAGCCAGGCGGACTACCTCAACCTGGTGCAGTGCGAGACGCTCGAGGACTTGAAACT
  GCACCTGCAGAGTACAGATTATGGCAACTTCCTGGCCAATGAAGCGTCACCTCTGACGGTGTCAGTCATC
  GATGACAAGCTCAAGGAGAAGATGGTAGTAGAGTTCCGCCACATGAGAAACCATGCCTACGAGCCACTTG
  CCAGCTTCCTGGACTTCATTACTTACAGCTACATGATTGACAACGTGATCCTGCTCATCACGGGCACACT
  GCACCAGCGTTCAATAGCTGAACTTGTGCCCAAGTGCCATCCGCTAGGCAGTTTTGAGCAGATGGAGGCT
  GTGAACATCGCACAGACACCCGCAGAGCTCTACAATGCCATTCTGGTGGACACGCCCCTGGCGGCTTTTT
  TCCAGGACTGCATCTCAGAGCAGGACCTTGATGAGATGAACATTGAGATAATCCGAAACACGCTTTACAA
  GGCTTATCTGGAGTCCTTCTACAAGTTCTGTACTCTGTTAGGGGGAACCACAGCTGATGCCATGTGTCCT
  ATCCTAGAGTTTGAAGCAGATCGCCGAGCTTTCATCATCACCATCAACTCTTTCGGCACAGAGCTTTCCA
  AGGAGGACCGTGCCAAGCTCTTTCCACACTGTGGCCGACTCTACCCTGAGGGCTTGGCTCAGCTGGCTCG
  GGCTGATGACTATGAACAGGTCAAGAATGTAGCTGATTACTACCCGGAATACAAACTGCTTTTTGAGGGT
  GCAGGTAGCAATCCTGGAGACAAGACCCTGGAGGACCGATTCTTTGAACATGAGGTGAAGCTGAACAAGC
  TGGCCTTCCTGAACCAGTTCCACTTTGGTGTCTTTTATGCCTTTGTGAAGCTTAAGGAACAGGAATGTCG
  CAACATTGTGTGGATTGCTGAGTGCATTGCCCAGCGCCATCGCGCCAAGATCGACAACTATATCCCCATC
  TTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR209969 representing NM_001011927
  Red=Cloning site Green=Tags(s)
  
  MSFFPELYFNVDNGYLEGLVRGLKAGVLSQADYLNLVQCETLEDLKLHLQSTDYGNFLANEASPLTVSVI
  DDKLKEKMVVEFRHMRNHAYEPLASFLDFITYSYMIDNVILLITGTLHQRSIAELVPKCHPLGSFEQMEA
  VNIAQTPAELYNAILVDTPLAAFFQDCISEQDLDEMNIEIIRNTLYKAYLESFYKFCTLLGGTTADAMCP
  ILEFEADRRAFIITINSFGTELSKEDRAKLFPHCGRLYPEGLAQLARADDYEQVKNVADYYPEYKLLFEG
  AGSNPGDKTLEDRFFEHEVKLNKLAFLNQFHFGVFYAFVKLKEQECRNIVWIAECIAQRHRAKIDNYIPI
  F
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001011927
• ORF Size: 1053 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001011927.1, NP_001011927.1
• RefSeq Size: 1611 bp
• RefSeq ORF: 1056 bp
• Locus ID: 291969
• Cytogenetics: 19q12
• MW: 40.3 kDa