F2 (NM_022924) Rat Tagged ORF Clone

CAT#: RR210046

F2 (Myc-DDK-tagged ORF) - Rat coagulation factor II (thrombin) (F2), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: F2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR210046 representing NM_022924
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGCACGTCCGCGGCCTGGGCCTACCTGGCTGCCTGGCTCTGGCTGCCCTGGCTAGCCTGGTACACA
  GTCAGCATGTGTTCCTGGCCCCTCAGCAAGCACTGTCGCTGCTCCAGCGGGTCCGCAGAGCCAACAGCGG
  CTTCCTGGAGGAGCTCCGAAAGGGCAACCTGGAGCGTGAGTGCGTGGAAGAGCAGTGCAGCTATGAGGAG
  GCTTTTGAGGCCCTGGAGTCTCCCCAAGACACGGATGTGTTCTGGGCCAAGTACACAGTGTGTGATTCAG
  TGAGGAAACCTCGAGAGACCTTCATGGACTGTCTAGAAGGTCGCTGTGCTATGGATCTGGGTCTGAACTA
  CCATGGGAATGTGAGTGTCACCCATACCGGCATCGAGTGTCAGCTGTGGCGAAGCCGCTACCCACACAGG
  CCTGACATCAACTCCACCACCCATCCTGGGGCTGACCTGAAGGAGAACTTCTGCCGAAATCCAGACAGCA
  GCACCTCAGGGCCCTGGTGCTACACCACAGACCCTACTGTGCGCAGAGAGGAATGCAGTATCCCTGTCTG
  TGGTCAGGAGGGCCGTACCACTGTGAAAATGACTCCTCGCTCAAGAGGTTCCAAGGAGAATCTGTCGCCT
  CCATTGGGAGAGTGCCTCCTCGAGCGTGGCCGGCTGTACCAGGGGAACTTAGCTGTGACAACACTTGGGT
  CCCCCTGCCTGGCCTGGGACAGTTTGCCAACCAAGACCCTGAGCAAGTACCAGAACTTCGACCCTGAGGT
  GAAACTCGTGCAAAACTTCTGCCGCAATCCAGACCGGGATGAGGAGGGCGCGTGGTGCTTTGTTGCGCAG
  CAGCCTGGTTTTGAATACTGCAGCCTCAACTACTGTGATGAAGCGGTGGGAGAGGAGAACCATGACGGGG
  ACGAGTCCATCGCAGGACGCACCACCGACGCGGAGTTCCACACCTTCTTCGATGAGAGGACTTTCGGCCT
  TGGGGAGGCAGACTGTGGCCTGCGGCCTTTGTTCGAGAAGAAGTCCTTGACAGACAAAACGGAAAAGGAG
  CTTCTTGATTCTTACATAGATGGGCGCATCGTGGAGGGCTGGGACGCTGAGAAGGGTATCGCCCCATGGC
  AGGTGATGCTTTTTCGGAAGAGTCCCCAAGAGCTGCTGTGTGGAGCCAGCCTTATCAGTGATCGATGGGT
  CCTCACTGCTGCCCACTGCATTCTGTACCCACCCTGGGACAAGAACTTCACTGAGAATGACCTCCTGGTG
  CGCATTGGCAAGCACTCCAGAACCAGATATGAGCGGAATGTTGAAAAGATCTCCATGCTGGAAAAGATCT
  ACATCCACCCCAGATATAACTGGCGGGAAAACCTAGACCGTGACATTGCTCTGCTCAAGCTAAAGAAGCC
  AGTGCCCTTCAGTGACTACATTCACCCCGTGTGCTTGCCAGACAAGCAGACAGTAACCAGTTTGCTCCAG
  GCTGGTTATAAAGGGCGGGTGACAGGCTGGGGCAACCTTCGGGAGACGTGGACAACTAACATCAACGAGA
  TACAGCCCAGCGTCCTGCAGGTGGTGAACCTGCCCATTGTAGAGCGACCAGTGTGCAAGGCCTCTACCCG
  GATACGCATTACTGACAACATGTTCTGTGCTGGCTTCAAGGTGAATGACACCAAGCGAGGAGATGCTTGT
  GAAGGTGACAGTGGGGGACCTTTTGTCATGAAGAGCCCCTACAACCACCGCTGGTACCAAATGGGTATTG
  TCTCCTGGGGTGAAGGCTGTGACCGGAATGGGAAATATGGCTTCTACACGCACGTGTTCCGTCTGAAAAG
  GTGGATGCAGAAAGTCATTGATCAGCATAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR210046 representing NM_022924
  Red=Cloning site Green=Tags(s)
  
  MSHVRGLGLPGCLALAALASLVHSQHVFLAPQQALSLLQRVRRANSGFLEELRKGNLERECVEEQCSYEE
  AFEALESPQDTDVFWAKYTVCDSVRKPRETFMDCLEGRCAMDLGLNYHGNVSVTHTGIECQLWRSRYPHR
  PDINSTTHPGADLKENFCRNPDSSTSGPWCYTTDPTVRREECSIPVCGQEGRTTVKMTPRSRGSKENLSP
  PLGECLLERGRLYQGNLAVTTLGSPCLAWDSLPTKTLSKYQNFDPEVKLVQNFCRNPDRDEEGAWCFVAQ
  QPGFEYCSLNYCDEAVGEENHDGDESIAGRTTDAEFHTFFDERTFGLGEADCGLRPLFEKKSLTDKTEKE
  LLDSYIDGRIVEGWDAEKGIAPWQVMLFRKSPQELLCGASLISDRWVLTAAHCILYPPWDKNFTENDLLV
  RIGKHSRTRYERNVEKISMLEKIYIHPRYNWRENLDRDIALLKLKKPVPFSDYIHPVCLPDKQTVTSLLQ
  AGYKGRVTGWGNLRETWTTNINEIQPSVLQVVNLPIVERPVCKASTRIRITDNMFCAGFKVNDTKRGDAC
  EGDSGGPFVMKSPYNHRWYQMGIVSWGEGCDRNGKYGFYTHVFRLKRWMQKVIDQHR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022924
• ORF Size: 1851 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_022924.1, NM_022924.2, NP_075213.2
• RefSeq Size: 2005
• RefSeq ORF: 1854
• Locus ID: 29251
• MW: 70.4 kDa
• Gene Summary: catalyzes the preferential cleavage of Arg-Gly; activates fibrinogen to fibrin and releases fibrinopeptides A and B; involved in blood coagulation and wound repair [RGD, Feb 2006]