Oas2 (NM_001009715) Rat Tagged ORF Clone

CAT#: RR210052

Oas2 (Myc-DDK-tagged ORF) - Rat 2'-5'-oligoadenylate synthetase 2, 69/71kDa (Oas2), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Oas2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR210052 representing NM_001009715
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAAACTGGATGCCTGGCTGGTCATCCAGTGGGTCTTTGGGGGTGCCCCCCATGCCAGTGCAGAAGC
  TAGAAAAGTCTGTCCAGGTAAACCTTGAACCAGATGAAAAATGTCTGAGTCAGACCGAGGTGTCCTCTGT
  ACCATCCCAGAAGCTAGAAGAATATATCCAGGCAAACCTCAAACCCGATGAAGAATCTCTGAAGCAGATA
  GACCAGGCTGTGGATGCCATCTCTGACCTGCTGTGCAGTGAGGTGATGATCGATGTGCTGAAAGTGGTTA
  AGGGTGGCTCCTATGGTCGGAAAACAGTCCTAAGAGACTGCTCCGATGGTACACTTGTTCTCTTCACCGG
  TCTCTTCAAACAGTTCCAAGACCAGAAGAAATACCAAGATAAGCTCCTTGACTTGATTGAACAACGGCTG
  AAAAGCCATGAGAAATACAAGAAGTCAGTAAAACGTAAACTTAGCCTCCTTGAAGTACAAGTGTCTATAC
  CAGGGCAGAGTATACTCTTGCAGTTGCTTCCAACCTTCAATCCTCTGTGCATCAGTGAGAATCCCAGCGC
  CCAGGTCTATCAGAATCTCAAAAGATCCATGGATCAAGTAAAAGCATCACCTGGGGAATTCTCAGACTGC
  TTCACCACACTGCAGCAGAGGTTTTTCGAGAAATATCCCGGGAGACTGAAGGATTTGATTCTATTGGTCA
  AGCACTGGTATAAACAGTTGCAGGATAAGTGGATAATACCCTCACCTCCGCCATTGCTATATGCACTGGA
  GCTGCTTACTGTGTATGCCTGGGAACAGGGCTGCCAGACTAAAGACTTTGACATCACGCAAGGTATCAGG
  ACTGTGCTGCAACTCATCAGTCAGCCGACAAACCTGTGTGTCTACTGGTTAGACAATTACAACTTTGAGG
  ATGAGACAGTCCGGAACAACCTTCTGCACCAGCTCAACTCCCCAAGACCGGTCATCTTGGATCCAACCGA
  CCCAACCAACAATGTGGGCAAAGATGACAGGTTCTGGCAGCTACTGGCAGAAGAGGCTCAGGAGTGGCTG
  AACTCTCTCAGACTGAATAAGCCACACAAACCATGTTGGGATGTTCTGCCCATGCCATTTTTCATCACCC
  CAAGCCACTGCCTGGACAAGTTCATCAAAGACTTCCTCCAACCTGACAAGGTCTTCCTAAACCAAATCAA
  AAGAGCTGTTGACATTATCTGTTCATTCTTAAAAGAGACCTGCTTCCAGAATTCTGACATCAAAGTCCTG
  AAGATCATCAAGGGAGGATCCACTGCCAAAGGCACAGCTCTGCAGCAGAGATCAGATGCTGACATCATAG
  TGTTCCTCAGCTCACTGGATAGTTATGACTCCCTAGAAACTGAACGCTCCCAGTACGTCCAGGAGATCCG
  AAAGCAGTTAGAAGCCTGCCAGAAGGCCTTTAATTTAGGGGTGAAGTTTGATATTTCCAAATGGATGGCC
  CCCAGGGTGCTGAGTTTTACCCTGGAATCCAAGAGTCTCAAGCAAAGTGTGGAGTTCGATGTCCTTCCCG
  CCTATGATGCACTAGGTCAGCTGCGGTCTGACTACACCTCCAGGCTCAAAGCCTACAAGAAGCTCATTGA
  GCTGTATGCCTCACAGGACAGCCTCAAAGGAGGGGAGTTTTCAGTCTGTTTTACAGAGCTACAGAGAGAC
  TTCATTGAAACCAGGCCCACCAAACTCAAGGGTCTGATCCGCCTGATCAAGCACTGGTACAAACAGTGTG
  AAAGGAAGATGAAGCCAAAAGCATCTTTGCCTCCAAAGTACGCACTGGAGCTGCTCACCGTGTATGCGTG
  GGAGCATGGCAGTGGCACAGATGGCTTCGACACTGCTGAAGGCTTCCGGACCGTCCTGGACTTGGTCATA
  AGATACCGGCAGCTCTGCGTCTTCTGGACAGTCAATTACAACTTTGAGGAGGATCACATGAGGAAGTTCC
  TACTGACCCAGATCCAGAAAAAGAGGCCTGTGATCCTGGATCCAGCAGATCCCACAGGTGATGTGGGAGG
  AGGTGACCGCTGGTGTTGGCATCTTCTAGCCAAAGAAGCAAAGGAGTGGCTGTCCTCCTCCTGTTTCCAA
  GTGGAGCCAAAAAGCCCCGTGCAGCCGTGGAAAGTACCAGTAGTACAGACTCCAGGAAGCTGTGGAGCTC
  AGATCTACCCTGTGGTGGGTGGGGTGTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR210052 representing NM_001009715
  Red=Cloning site Green=Tags(s)
  
  MGNWMPGWSSSGSLGVPPMPVQKLEKSVQVNLEPDEKCLSQTEVSSVPSQKLEEYIQANLKPDEESLKQI
  DQAVDAISDLLCSEVMIDVLKVVKGGSYGRKTVLRDCSDGTLVLFTGLFKQFQDQKKYQDKLLDLIEQRL
  KSHEKYKKSVKRKLSLLEVQVSIPGQSILLQLLPTFNPLCISENPSAQVYQNLKRSMDQVKASPGEFSDC
  FTTLQQRFFEKYPGRLKDLILLVKHWYKQLQDKWIIPSPPPLLYALELLTVYAWEQGCQTKDFDITQGIR
  TVLQLISQPTNLCVYWLDNYNFEDETVRNNLLHQLNSPRPVILDPTDPTNNVGKDDRFWQLLAEEAQEWL
  NSLRLNKPHKPCWDVLPMPFFITPSHCLDKFIKDFLQPDKVFLNQIKRAVDIICSFLKETCFQNSDIKVL
  KIIKGGSTAKGTALQQRSDADIIVFLSSLDSYDSLETERSQYVQEIRKQLEACQKAFNLGVKFDISKWMA
  PRVLSFTLESKSLKQSVEFDVLPAYDALGQLRSDYTSRLKAYKKLIELYASQDSLKGGEFSVCFTELQRD
  FIETRPTKLKGLIRLIKHWYKQCERKMKPKASLPPKYALELLTVYAWEHGSGTDGFDTAEGFRTVLDLVI
  RYRQLCVFWTVNYNFEEDHMRKFLLTQIQKKRPVILDPADPTGDVGGGDRWCWHLLAKEAKEWLSSSCFQ
  VEPKSPVQPWKVPVVQTPGSCGAQIYPVVGGVY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001009715
• ORF Size: 2199 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001009715.1, NP_001009715.1
• RefSeq Size: 2274 bp
• RefSeq ORF: 2202 bp
• Locus ID: 363938
• Cytogenetics: 12q16
• MW: 84.1 kDa