Gpatch2 (NM_001011909) Rat Tagged ORF Clone

CAT#: RR210204

Gpatc2 (Myc-DDK-tagged ORF) - Rat G patch domain containing 2 (Gpatc2), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gpatch2
• Synonyms: Gpatc2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR210204 representing NM_001011909
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTGGGGCCGACGGGCGCCCAGCGATCGGAGCCGCCGCCGCCGCCCGGAAGAGCTGGCATTTCAGTC
  GAACAATGGAAGAGCTTGTTCACGATCTTGTCTCTGCACTGGAAGAGAGCTCTGAGCAAGCCCGAGGTGG
  TTTTGCTGAAACGGGAGATCATTCTCGAAATCTGTCTTGCCCCCTGAAGCGCCAGGCCAGGAAGAGGAGA
  GGGAGGAAGCGGAGATCCTACAATGTTCACCACCCCTGGGAGACTGGCCACTGCTTAAGCGAAGGCTCGG
  ATTCTAGTTTAGAGGAACCAAGTAAGGACTACAGAGAGAAGCACAGCAACAACAAAAAGGACCGCAGTGA
  CTCCGATGACCAGATGTTAGTGGCGAAGCGGAGGCCGTCCTCCACCTTAAACAACAGTGTTCGAGGTAAG
  CGACTTCTGTGGCATGAGTCTGACTTCGCGGTGGACAACCTTGGGAACAGAACTCTCCGCCGGAGGAGGA
  AGGTGAAGCGCATGGCGGTGGATCTCCCGCAGGACATCTCCACCAAAAGGACGATGACCCAGCCGCCTGA
  TGGCTGTAGAGACCAGGACATGGACAGCGATGGAGCCAGCCAGTATCCCGAGTTTATCCGCAGTAAAGTC
  AAAAAGCGGAGGCTGAAGGTGATCAGACCGAAAGCCCCGGAGGAAGGAGCGGTGTTGGAGAGTGAAGAAG
  TAAGCCAGGCCGACAAGGACAAGATGGAGTATGTGGAACCGAAAGCCTCAGATGAGCTCAGGAGTGAAAG
  TGACTCCAGCAGCCTCAGCAGCACTGATGCCGGCCTGTTCACCAACGATGAGGGAAGACAAGTTTTCATC
  TTAATAGTAAGAAATTTCAAAGTCAGGAGTCCTTCAAAGGGCAAGCTCATGAGTGGAAACTCTGCCAAGC
  AAGCCACAGCACTCGAGGCTCCTTGGGATCTCAGGCACAGTGACGATGAGCAGAGCGACTGGTTCTATGA
  GAAGGAGTCGGGCGGAGCCTGTGGAATTGCTGGAGTCGCGCCCTGGTGGGAAGAGGAAGGCCCCGTAGAG
  CTGGACACCAACCTCCCCGACCCCGTGTTTGAGAGCATCTTAAGTGGCTCCTTCCCTCTCATGTCACATC
  CTGGCAGAAGAGGTTTCCAAGCTAGACTCAGTTGCCTTCATGGAGTGCCTTCAAAGAATATTAAGAAATC
  TGCAGGGGCTCCACCTTCAATGGTAGGCATGCTTTTTTAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR210204 representing NM_001011909
  Red=Cloning site Green=Tags(s)
  
  MFGADGRPAIGAAAAARKSWHFSRTMEELVHDLVSALEESSEQARGGFAETGDHSRNLSCPLKRQARKRR
  GRKRRSYNVHHPWETGHCLSEGSDSSLEEPSKDYREKHSNNKKDRSDSDDQMLVAKRRPSSTLNNSVRGK
  RLLWHESDFAVDNLGNRTLRRRRKVKRMAVDLPQDISTKRTMTQPPDGCRDQDMDSDGASQYPEFIRSKV
  KKRRLKVIRPKAPEEGAVLESEEVSQADKDKMEYVEPKASDELRSESDSSSLSSTDAGLFTNDEGRQVFI
  LIVRNFKVRSPSKGKLMSGNSAKQATALEAPWDLRHSDDEQSDWFYEKESGGACGIAGVAPWWEEEGPVE
  LDTNLPDPVFESILSGSFPLMSHPGRRGFQARLSCLHGVPSKNIKKSAGAPPSMVGMLFY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001011909
• ORF Size: 1230 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001011909.1, NP_001011909.1
• RefSeq Size: 1392 bp
• RefSeq ORF: 1233 bp
• Locus ID: 289362
• Cytogenetics: 13q26
• MW: 45.9 kDa