Krt28 (NM_001008760) Rat Tagged ORF Clone

CAT#: RR210239

Krt28 (Myc-DDK-tagged ORF) - Rat keratin 28 (Krt28), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Krt28
• Synonyms: Ka41; Krt25d
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR210239 representing NM_001008760
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCTCCGGTTTTCTGGCGGATCCAGGCACGCTGGCCTACAGTCAGGATCCCTCAGGCCGCCCAGCG
  GAGGCGCAGGCTTTGCTGGGAGCAGTGCGGCCGGCGGTTCAGTTGCTGGTAGTGGATTTTCTTGGGCCTT
  GGGAGGGACACTGTGCAGTGCTCCTGGTGGGAGCCACGCTACTGGTGCCCTGGGAAATGTCACTTGTGTT
  GGCTTCGCTGGAAGTGAAGGAGGGCTCCTCTCTGGAAACGAGAAGGTCACCATGCAGAATCTCAACAACC
  GCTTGGCCTCTTACCTGGATAATGTGAAAGCTCTGGAGGAAGCGAACACTGAACTAGAGAGAAAGATCAA
  AACTTGGCATGAGAAATACGGACCCGGATCTTGCCGTGGGCTTGACCGTGACTACAGCAATTATCATCTA
  ACCATTGAGGACCTTAAAAGCAAGGTCATCTCTTCCACGGCTGCTAACGCTAATATAATTCTGCAGATTG
  ATAATGCCAGACTGGCTGCTGACGATTTCAGACTAAAGTATGAAAACGAACTCACCCTTCACCAAAATGT
  GGAGGCCGACATCAATGGATTGCGTCGCGTCCTGGACGAGCTGACGCTCTGCAGGACGGACCAAGAGCTG
  CAGTATGAATCCCTGAGTGAGGAGATGACTTACCTCAAGAAGAACCACGAAGAGGAAATGAAGGTTCTGC
  AGTGCGCAGCCGGAGGTAACGTGAATGTGGAGATGAACGCGGCCCCGGGCGTGGACCTCACCGTCCTGCT
  GAACAACATGAGAGCCGAGTACGAGGCCCTGGCTGAGCAGAACCGCAGGGACGCCGAGGCCTGGTTCCAA
  GAGAAGAGCGCCACGCTGCAGCAGCAGATCTCCAATGACTTGGGCGCAGCCACCTCAGCCAGGACTGAGC
  TGACAGAGCTGAAGCGCTCCCTGCAGACGCTGGAGATCGAGCTGCAGTCACTCTCAGCAACGGGTTTTGA
  ATCTGGAACCTCGGGGAATTCATCCAAAGATGTATCCAAAACCACACTGGTGAAGACCGTGGTTGAAGAA
  ATAGATCCGCGTGGTAAGGTCCTGTCCTCCAGGATCCACTCCATCGAGGAAAAGATGACCAAGATAAGCA
  ACAGCAAGGCTGAGCAGAGGGTTCCCTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR210239 representing NM_001008760
  Red=Cloning site Green=Tags(s)
  
  MSLRFSGGSRHAGLQSGSLRPPSGGAGFAGSSAAGGSVAGSGFSWALGGTLCSAPGGSHATGALGNVTCV
  GFAGSEGGLLSGNEKVTMQNLNNRLASYLDNVKALEEANTELERKIKTWHEKYGPGSCRGLDRDYSNYHL
  TIEDLKSKVISSTAANANIILQIDNARLAADDFRLKYENELTLHQNVEADINGLRRVLDELTLCRTDQEL
  QYESLSEEMTYLKKNHEEEMKVLQCAAGGNVNVEMNAAPGVDLTVLLNNMRAEYEALAEQNRRDAEAWFQ
  EKSATLQQQISNDLGAATSARTELTELKRSLQTLEIELQSLSATGFESGTSGNSSKDVSKTTLVKTVVEE
  IDPRGKVLSSRIHSIEEKMTKISNSKAEQRVPF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001008760
• ORF Size: 1149 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001008760.1, NP_001008760.1
• RefSeq Size: 1152 bp
• RefSeq ORF: 1152 bp
• Locus ID: 360623
• Cytogenetics: 10q31
• MW: 41.3 kDa