Prss23 (NM_001007691) Rat Tagged ORF Clone

CAT#: RR210287

Prss23 (Myc-DDK-tagged ORF) - Rat protease, serine, 23 (Prss23), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Prss23
• Synonyms: MGC94216
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR210287 representing NM_001007691
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGGAATCCCAGGACTCCTCATCCTTCTCCTTGTCCTGCTCTGTGTGTTCATGCAGGTGAGTCCCT
  ACAACGTTCCGTGGAAACCAACATGGCCGGCTTACCGCCTCCCTATAGTCTTGCCTCAGTCTACCCTCAA
  GTTAGCCAAACCAGACTTCGATGCCAAAGCAAAATTGGAGGTGTCCTCCTCATGTGGACCCCAGTGTCAC
  AAGGGAACACCGCTGCCCACCTACGAAGAGGCCAAGCAGTACCTTTCCTATGAAACCCTTTATGCCAACG
  GCAGCCGCACAGAGACTCAGGTGGGCATCTACATCCTCAGCAACGGTGAAGGCAGGGCACGCAGCAGAGA
  CTCGGAGGCCGCAGGGAAATCTCGCAGGAAGAGGCAGATTTATGGCTATGATGGCAGATTTAGCATTTTC
  GGGAAGGACTTCCTGCTCAACTATCCCTTCTCAACATCGGTGAAGTTGTCTACTGGCTGCACCGGCACCC
  TGGTGGCAGAGAAGCACGTCCTCACTGCTGCCCACTGCATACACGATGGCAAAACCTATGTGAAAGGAAC
  ACAGAAACTCCGAGTGGGCTTCCTGAAGCCCAAGTATAAAGATGGTGCCGGAGGGGACAACAGTTCAAGC
  TCAGCTCTGGTAGAGAAGATGAAATTTCAGTGGATCCGTGTGAAACGCACCCATGTGCCCAAGGGGTGGA
  TCAAGGGCAATGCCAACGACATTGGCATGGATTATGACTACGCCCTTCTGGAACTCAAGAAACCCCACAA
  AAGAAAGTTCATGAAGATTGGTGTGAGTCCTCCAGCGAAGCAGCTCCCGGGGGGCAGGATCCACTTCTCA
  GGCTACGACAATGACCGGCCCGGCAATTTGGTGTACCGCTTCTGTGATGTCAAAGATGAGACATACGACC
  TTCTTTATCAGCAGTGTGATGCCCAGCCCGGGGCCAGTGGTTCAGGGGTCTATGTGAGGATGTGGAAGAG
  ACCACAGCAGAAATGGGAAAGGAAAATTATCGGCATCTTTTCAGGGCACCAGTGGGTGGACATGAATGGC
  TCTCCGCAGGATTTCAACGTGGCAGTTAGAATCACACCTCTTAAATATGCCCAGATTTGCTATTGGATTA
  AAGGAAACTACCTGGATTGCAGGGAGGGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR210287 representing NM_001007691
  Red=Cloning site Green=Tags(s)
  
  MAGIPGLLILLLVLLCVFMQVSPYNVPWKPTWPAYRLPIVLPQSTLKLAKPDFDAKAKLEVSSSCGPQCH
  KGTPLPTYEEAKQYLSYETLYANGSRTETQVGIYILSNGEGRARSRDSEAAGKSRRKRQIYGYDGRFSIF
  GKDFLLNYPFSTSVKLSTGCTGTLVAEKHVLTAAHCIHDGKTYVKGTQKLRVGFLKPKYKDGAGGDNSSS
  SALVEKMKFQWIRVKRTHVPKGWIKGNANDIGMDYDYALLELKKPHKRKFMKIGVSPPAKQLPGGRIHFS
  GYDNDRPGNLVYRFCDVKDETYDLLYQQCDAQPGASGSGVYVRMWKRPQQKWERKIIGIFSGHQWVDMNG
  SPQDFNVAVRITPLKYAQICYWIKGNYLDCREG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001007691
• ORF Size: 1149 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001007691.1, NP_001007692.1
• RefSeq Size: 1877 bp
• RefSeq ORF: 1152 bp
• Locus ID: 308807
• Cytogenetics: 1q32
• MW: 43.2 kDa