Npffr1 (NM_022291) Rat Tagged ORF Clone

CAT#: RR210301

Npffr1 (Myc-DDK-tagged ORF) - Rat neuropeptide FF receptor 1 (Npffr1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Npffr1
• Synonyms: Gpr147; NPFF1; OT7T022
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR210301 representing NM_022291
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCGGAGCCCTCCCAGCCTCCCAACGGCAGCTGGCCCCTGGGTCAGAACGGGAGTGATGTGGAGA
  CCAGCATAGCAACCAGCCTCACCTTCTCCTCCTACTACCAACACTCCTCTCCGGTGGCAGCCATGTTCAT
  CGCGGCCTACGTGCTCATCTTCCTCCTCTGCATGGTGGGCAACACCCTGGTCTGCTTCATTGTGCTCAAG
  AACCGGCACATGCGCACTGTCACCAACATGTTTATCCTCAACCTGGCCGTCAGCGACCTGCTGGTGGGCA
  TCTTCTGCATGCCCACAACCCTTGTGGACAACCTTATCACTGGTTGGCCTTTTGACAACGCCACATGCAA
  GATGAGCGGCTTGGTGCAGGGCATGTCCGTGTCTGCATCGGTTTTCACACTGGTGGCCATCGCTGTGGAA
  AGGTTCCGCTGCATCGTGCACCCTTTCCGCGAGAAGCTGACCCTTCGGAAGGCGCTGTTCACCATCGCGG
  TGATCTGGGCTCTGGCGCTGCTCATCATGTGTCCCTCGGCGGTCACTCTGACAGTCACCCGAGAGGAGCA
  TCACTTCATGCTGGATGCTCGTAACCGCTCCTACCCGCTCTACTCGTGCTGGGAGGCCTGGCCCGAGAAG
  GGCATGCGCAAGGTCTACACCGCGGTGCTCTTCGCGCACATCTACCTGGTGCCGCTGGCGCTCATCGTAG
  TGATGTACGTGCGCATCGCGCGCAAGCTATGCCAGGCCCCCGGTCCTGCGCGCGACACGGAGGAGGCGGT
  GGCCGAGGGTGGCCGCACTTCGCGCCGTAGGGCCCGCGTGGTGCACATGCTGGTCATGGTGGCGCTCTTC
  TTCACGTTGTCCTGGCTGCCACTCTGGGTGCTGCTGCTGCTCATCGACTATGGGGAGCTGAGCGAGCTGC
  AACTGCACCTGCTGTCGGTCTACGCCTTCCCCTTGGCACACTGGCTGGCCTTCTTCCACAGCAGCGCCAA
  CCCCATCATCTACGGCTACTTCAACGAGAACTTCCGCCGCGGCTTCCAGGCTGCCTTCCGTGCACAGCTC
  TGCTGGCCTCCCTGGGCCGCCCACAAGCAAGCCTACTCGGAGCGGCCCAACCGCCTCCTGCGCAGGCGGG
  TGGTGGTGGACGTGCAACCCAGCGACTCCGGCCTGCCATCAGAGTCTGGCCCCAGCAGCGGGGTCCCAGG
  GCCTGGCCGGCTGCCACTGCGAAATGGGCGTGTGGCCCATCAGGATGGCCCGGGGGAAGGGCCAGGCTGC
  AACCACATGCCCCTCACCATCCCGGCCTGGAACATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR210301 representing NM_022291
  Red=Cloning site Green=Tags(s)
  
  MEAEPSQPPNGSWPLGQNGSDVETSIATSLTFSSYYQHSSPVAAMFIAAYVLIFLLCMVGNTLVCFIVLK
  NRHMRTVTNMFILNLAVSDLLVGIFCMPTTLVDNLITGWPFDNATCKMSGLVQGMSVSASVFTLVAIAVE
  RFRCIVHPFREKLTLRKALFTIAVIWALALLIMCPSAVTLTVTREEHHFMLDARNRSYPLYSCWEAWPEK
  GMRKVYTAVLFAHIYLVPLALIVVMYVRIARKLCQAPGPARDTEEAVAEGGRTSRRRARVVHMLVMVALF
  FTLSWLPLWVLLLLIDYGELSELQLHLLSVYAFPLAHWLAFFHSSANPIIYGYFNENFRRGFQAAFRAQL
  CWPPWAAHKQAYSERPNRLLRRRVVVDVQPSDSGLPSESGPSSGVPGPGRLPLRNGRVAHQDGPGEGPGC
  NHMPLTIPAWNI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022291
• ORF Size: 1296 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_022291.1, NM_022291.2, NP_071627.2
• RefSeq Size: 1307
• RefSeq ORF: 1299
• Locus ID: 64107
• MW: 48.3 kDa
• Gene Summary: a G protein-coupled receptor that binds neuropeptide FF and BIBP3226, an anorexigenic Y1 receptor ligand and has a potential role in feeding [RGD, Feb 2006]