Gpbar1 (NM_177936) Rat Tagged ORF Clone

CAT#: RR210451

Gpbar1 (Myc-DDK-tagged ORF) - Rat G protein-coupled bile acid receptor 1 (Gpbar1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gpbar1
• Synonyms: Tgr5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR210451 representing NM_177936
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGTCACACAACACCACTGAGCTGTCAGCCATTCCCAGAGGGGTTCAGGAGCTTTCCCTGGTCCTGG
  CAAGCCTCATCGTCATCGCCAACCTGCTCCTGGCCCTAGGCATTGTCCTGGACCGCCACTTACGCAGCCC
  ACCTGCTGGCTGCTTCTTTCTAAGCCTACTACTAGCTGGGCTACTCACAGGGTTGGCACTGCCCACGCTG
  CCTGGGCTATGGAATAGGAGCCATCAGGGGTACTGGTCCTGCCTCCTTCTCCACTTGGCCCCCAACTTTT
  GTTTCCTCTCCCTGCTTGCCAATCTGCTGCTGGTGCATGGGGAACGCTACATGGCAGTGTTGCAGCCACT
  CCGGCCCCATGGGAGTGTGCGGCTAGCCCTGTTCCTCACCTGGATCAGCTCCCTGCTCTTTGCCAGCCTG
  CCTGCTCTGGGCTGGAACCACTGGAGTCCTGGTGCCAACTGCAGCTCCCAGGCTATCTTCCCAGCCCCCT
  ACCTTTACCTCGAAGTCTATGGGCTCCTGCTGCCCGCTGTGGGGGCCACTGCCCTTCTCTCTGTCCGAGT
  GTTGGCCACTGCCCACCACCAGCTGCGGGAGATCCGCAGACTGGAGCGGGCGGTGTGCCGTGATGCACCC
  TCAACCCTAGCGAGGGCTCTCACCTGGAGGCAGGCTAGGGCACAGGCAGGAGCCACACTGCTCTTTTTGC
  TGTGTTGGGGGCCCTATGTGGCCACATTGCTCCTGTCAGTCTTGGCCTATGAGCGGCGGCCACCACTAGG
  GCCTGTAACTCTGTTATCTCTCATCTCATTGGGCAGTGCCAGTGCTGCAGTTGTGCCTGTGGCCATGGGT
  CTGGGTGATCAGCGCTACACGGCCCCCTGGAGGACAGCTGCCCAAAGGTGGCTACAAGTGCTTCGAGGAA
  GACCCAAGAGGGCCAATCCAGGCCCCAGCACTGCCTACCACTCCAGTAGCCAATGCAGCACTGACTTGGA
  CTTGAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR210451 representing NM_177936
  Red=Cloning site Green=Tags(s)
  
  MMSHNTTELSAIPRGVQELSLVLASLIVIANLLLALGIVLDRHLRSPPAGCFFLSLLLAGLLTGLALPTL
  PGLWNRSHQGYWSCLLLHLAPNFCFLSLLANLLLVHGERYMAVLQPLRPHGSVRLALFLTWISSLLFASL
  PALGWNHWSPGANCSSQAIFPAPYLYLEVYGLLLPAVGATALLSVRVLATAHHQLREIRRLERAVCRDAP
  STLARALTWRQARAQAGATLLFLLCWGPYVATLLLSVLAYERRPPLGPVTLLSLISLGSASAAVVPVAMG
  LGDQRYTAPWRTAAQRWLQVLRGRPKRANPGPSTAYHSSSQCSTDLDLN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_177936
• ORF Size: 987 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_177936.1, NP_808797.1
• RefSeq Size: 990
• RefSeq ORF: 990
• Locus ID: 338443
• MW: 35.8 kDa
• Gene Summary: a bile acid receptor [RGD, Feb 2006]