Gclm (NM_017305) Rat Tagged ORF Clone

CAT#: RR210532

Gclm (Myc-DDK-tagged ORF) - Rat glutamate cysteine ligase, modifier subunit (Gclm), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gclm
• Synonyms: Glclr
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR210532 representing NM_017305
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCACCGACAGCCGCGCGGCCGGAGCACTTCTGGCGCGGGCCAGCACCCTGCACCTGCAGACCGGGA
  ACCTGCTCAACTGGGGCCGCCTGCGGAAAAAGTGTCCGTCCACGCACAGCGAGGAGCTTCGAGACTGTAT
  CCAAAAGACCTTGAATGAATGGAGCTCCCAAATCAGCCCTGATTTGGTCAGGGAGTTTCCAGATGTTTTG
  GAATGTACCATGTCCCATGCAGTGGAAAAGATAAACCCTGATGAAAGAGAAGAAATGAAAGTTTCTGCTA
  AACTGTTCATTGTAGGATCGAATTCTTCATCATCAACTAGAAATGCAGTTGACATGGCATGCTCAGTCCT
  TGGAGTTGCACAGCTGGACTCTGTCATCATGGCTTCCCCTCCAATTGAAGATGGAGTTAATCTTTCCTTG
  GAGCATTTGCAGCCTTACTGGGAGGAATTAGAAAACTTAGTTCAGAGCAAGAAGATTGTTGCTATAGGCA
  CCTCGGATCTAGACAAAACACAGTTGGAGCAGCTGTACCAGTGGGCACAGGTAAAACCCAATAGTAATCA
  AGTTAATCTTGCCTCCTGCTGTGTGATGCCACCAGATTTGACTGCATTTGCTAAACAGTTTGACATACAG
  CTACTGACTCACAATGACCCAAAAGAACTGCTCTCTGAGGCAAGTTTCCAGGAAGCTCTTCAAGAAAGCA
  TCCCTGACATTGAAGCCCAGGAGTGGGTGCCACTGTGGCTGCTGAGGTACTCGGTCATCGTGAAAAGCAG
  AGGAATCATCAAGTCAAAAGGATACATTTTGCAAGCCAAAAGAAAGGGTTCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR210532 representing NM_017305
  Red=Cloning site Green=Tags(s)
  
  MGTDSRAAGALLARASTLHLQTGNLLNWGRLRKKCPSTHSEELRDCIQKTLNEWSSQISPDLVREFPDVL
  ECTMSHAVEKINPDEREEMKVSAKLFIVGSNSSSSTRNAVDMACSVLGVAQLDSVIMASPPIEDGVNLSL
  EHLQPYWEELENLVQSKKIVAIGTSDLDKTQLEQLYQWAQVKPNSNQVNLASCCVMPPDLTAFAKQFDIQ
  LLTHNDPKELLSEASFQEALQESIPDIEAQEWVPLWLLRYSVIVKSRGIIKSKGYILQAKRKGS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017305
• ORF Size: 822 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_017305.1, NM_017305.2, NP_059001.1
• RefSeq Size: 1524
• RefSeq ORF: 825
• Locus ID: 29739
• MW: 30.5 kDa
• Gene Summary: an integral part of the enzyme gamma-glutamylcysteine synthetase, which catalyzes the first step of glutathione (GSH) synthesis [RGD, Feb 2006]