Pld2 (NM_033299) Rat Tagged ORF Clone

CAT#: RR210552

Pld2 (Myc-DDK-tagged ORF) - Rat phospholipase D2 (Pld2), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pld2
• Synonyms: Pldc
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR210552 representing NM_033299
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTGTAACCCAGACGGACCTCTTTCCCTATGGGGACTACCTGAACTCCAGCCAGTTGCACATGGAGC
  CAGATGAGGTTGACACTCTGAAGGAAGGAGAGGATCCAGCTGATCGAATGCATCCCTTTCTGGCCATCTA
  TGACCTTCAGCCTCTGAGAGCACACCCCTTGGTGTTTGCTCCTGGGGTCCCTGTTATAGCCCAGGTGGTA
  GGCACCGAAAGATACACCAGCGGATCCAAGGTGGGAACCTGTACTCTTTATTCTGTTCGATTGACCCATG
  GCGACTTTACCTGGACAACCAAGAAGAAATTCCGACACTTTCAGGAGCTGCATCGGGACCTCCAGAGACA
  CAAAGTCTTGATGAGTCTGCTCCCTCTGGCTCGCTTTGCTGCGGCCCATTCTCCAGCCCGAGAGGCAGCC
  AATGAGAATATTCCCTCCCTACCCCGAGGAGGTTCCGAGGGCTCTGCCAGACACACAGCCAGCAAGCAGA
  AGTACCTGGAAAATTACCTCAACCGCCTCCTGACCATGTCTTTCTATCGAAATTACCATGCCATGACAGA
  ATTTCTGGAAGTCAGTCAACTTTCCTTTATCCCAGACCTTGGCTCCAAAGGACTGGAAGGGGTGATCCGG
  AAGCGCTCAGGTGGGCATCGAGTTCCTGGCTTCACCTGCTGTGGTCGAGACCAAGTTTGTTATCGATGGT
  CCAAGAGGTGGCTGGTGGTGAAGGACTCCTTCCTGCTGTACATGCGTCCAGAGACTGGCGCCATCTCATT
  TGTTCAACTTTTTGACCCTGGCTTTGAGGTCCAGGTGGGGAAAAGGAGCACAGAGGCACGGTATGGGGTG
  AGGATCGACACCTCCCACAGGTCCCTGATCCTCAAATGCAGCAGCTACCGGCAGGCACGGTGGTGGGGCC
  AGGAGATCACGGAGCTGGCACAGGGTCCGGGCAGAGATTTTCTACAGCTACACCAGCATGACAGCTATGC
  CCCACCCCGGCCTGGCACCCTGGCCCGGTGGTTTGTGAATGGGGCAGGTTACTTTGCTGCTGTGGCAGAT
  GCCATCCTCCGAGCTCGAGAGGAGATTTTCATCACAGACTGGTGGCTGAGTCCTGAGATTTACCTGAAGC
  GTCCAGCCCACTCAGATGACTGGAGACTGGACATTATGCTCAAGAGGAAGGCGGAAGAAGGTGTCCGTGT
  TTCCATACTGCTGTTTAAGGAAGTGGAACTGGCCTTGGGCATCAACAGTGGCTATAGCAAGAGGACACTG
  ATGCTACTGCACCCCAACATAAAGGTGATGCGACACCCAGACCTTGTAACATTGTGGGCTCATCATGAGA
  AGCTCCTGGTGGTAGACCAAGCGGTGGCATTCTTGGGTGGGCTGGACCTTGCCTATGGCCGCTGGGATGA
  TGTGCAGTACCGACTGACTGACCTGGGGGACCCCTCCGAATCTGCAGATTCACAGACTCCCACGCCAGGT
  TCAGATCCTGCAGCCACTCCAGACCTCTCGCATAATCACTTCTTCTGGCTGGGAAAAGACTACAGCAACC
  TCATTACCAAGGACTGGGTACAGCTGGACCGGCCTTTTGAAGATTTCATCGACAGGGAGACCACACCCAG
  AATGCCATGGAGGGATGTTGGAGTGGTTGTACATGGAGTAGCTGCCCGGGACCTTGCCCGGCACTTCATC
  CAGCGCTGGAACTTCACCAAGACCATCAAGGCCAGATACAAGATACCTCAGTACCCCTACCTGCTGCCTA
  AGTCCGCCAGCACTGCAAACCATCTTCCCTTCATAATCCCAGGCGCGCAGTGCGCCACTGTGCAGGTCTT
  GCGGTCTGTGGATCGATGGTCAGCAGGGACTTTGGAGAGCTCCATCCTCAATGCCTACCTACATACCATC
  CGAGAGAGCCAGCACTTTCTCTACATTGAGAATCAGTTCTTCATCAGCTGCTCAGATGGGCGTACAGTTC
  TGAACAAGGTGGGCGATGAGATTGTGGACAGGATCCTAAAGGCTCATGAACAGGGGCAGTGTTTCCGAGT
  CTACGTGCTTCTGCCTTTGCTCCCTGGCTTTGAGGGGGACATCTCCACAGGGGGTGGCAACTCCATCCAG
  GCCATTCTGCACTTCACCTACAGGACCCTGTGTCGTGGGGAATATTCAATCTTACATCGTCTCAAAGCAG
  CCATGGGGACAGCATGGCGGGATTACATGTCCATCTGTGGGCTTCGCACACATGGAGAGCTGGGCGGGCA
  CCCGATCTCCGAGCTCATCTATATCCACAGCAAGTTGCTCATTGCAGATGACAGAACAGTCATCATCGGC
  TCTGCAAACATCAACGACAGGAGCTTGCTGGGGAAGCGCGACAGTGAGCTAGCCATACTGATCGAGGACA
  CAGAAATGGAGCCATCCCTCATGGATGGGGTGGAGTACCAGGCAGGCAGATTTGCCTTGAGTCTGCGGAA
  GCACTGTTTCAGTGTCATTCTTGGGGCAAATACCTGGCCAGACCTGGATCTCCGAGACCCTGTCTGTGAT
  GACTTCTTCCAGCTGTGGCAGGAAACAGCGGAGAACAATGCCACCATCTATGAGCAGATCTTCCGCTGCC
  TGCCGTCCAATGCTACTCGTTCCCTGCGGGCTCTCCGGGAGTACGTGGCTGTGGAGTCCTTGGCTACAGT
  CAGCCCTTCTTTGGCTCAGTCTGAGCTTGCCCACATCCGGGGCCACTTAGTTCACTTCCCCCTCAAGTTT
  CTGGAGGATGAGTCCTTGTTGCCTCCACTGGGGAGCAAAGAGGGGATGATACCTTTAGAAGTGTGGACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR210552 representing NM_033299
  Red=Cloning site Green=Tags(s)
  
  MTVTQTDLFPYGDYLNSSQLHMEPDEVDTLKEGEDPADRMHPFLAIYDLQPLRAHPLVFAPGVPVIAQVV
  GTERYTSGSKVGTCTLYSVRLTHGDFTWTTKKKFRHFQELHRDLQRHKVLMSLLPLARFAAAHSPAREAA
  NENIPSLPRGGSEGSARHTASKQKYLENYLNRLLTMSFYRNYHAMTEFLEVSQLSFIPDLGSKGLEGVIR
  KRSGGHRVPGFTCCGRDQVCYRWSKRWLVVKDSFLLYMRPETGAISFVQLFDPGFEVQVGKRSTEARYGV
  RIDTSHRSLILKCSSYRQARWWGQEITELAQGPGRDFLQLHQHDSYAPPRPGTLARWFVNGAGYFAAVAD
  AILRAREEIFITDWWLSPEIYLKRPAHSDDWRLDIMLKRKAEEGVRVSILLFKEVELALGINSGYSKRTL
  MLLHPNIKVMRHPDLVTLWAHHEKLLVVDQAVAFLGGLDLAYGRWDDVQYRLTDLGDPSESADSQTPTPG
  SDPAATPDLSHNHFFWLGKDYSNLITKDWVQLDRPFEDFIDRETTPRMPWRDVGVVVHGVAARDLARHFI
  QRWNFTKTIKARYKIPQYPYLLPKSASTANHLPFIIPGAQCATVQVLRSVDRWSAGTLESSILNAYLHTI
  RESQHFLYIENQFFISCSDGRTVLNKVGDEIVDRILKAHEQGQCFRVYVLLPLLPGFEGDISTGGGNSIQ
  AILHFTYRTLCRGEYSILHRLKAAMGTAWRDYMSICGLRTHGELGGHPISELIYIHSKLLIADDRTVIIG
  SANINDRSLLGKRDSELAILIEDTEMEPSLMDGVEYQAGRFALSLRKHCFSVILGANTWPDLDLRDPVCD
  DFFQLWQETAENNATIYEQIFRCLPSNATRSLRALREYVAVESLATVSPSLAQSELAHIRGHLVHFPLKF
  LEDESLLPPLGSKEGMIPLEVWT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_033299
• ORF Size: 2799 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_033299.2, NP_150641.2
• RefSeq Size: 4562
• RefSeq ORF: 2802
• Locus ID: 25097
• MW: 106 kDa
• Gene Summary: catalyzes hydrolysis of phosphatidylcholine (PC) to phosphatidic acid and choline; involved in important cellular processes such as signal transduction, membrane trafficking, and mitosis regulation [RGD, Feb 2006]