Chrnb1 (NM_012528) Rat Tagged ORF Clone

CAT#: RR210553

Chrnb1 (Myc-DDK-tagged ORF) - Rat cholinergic receptor, nicotinic, beta 1 (muscle) (Chrnb1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Chrnb1
• Synonyms: Acrb; nAChR; RNACRB1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR210553 representing NM_012528
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTTAGGGGCGCTGCTTCTGATTCTGGGGATACTCGGGACGCCCCTCGCCCCAGGAGCCCGCGGGT
  CGGAAGCCGAAGGCCAACTGCTTAAGAAACTTTTCTCGGATTATGATAGCTCAGTAAGGCCGGCGCAGGA
  GGTGGGAGACCGCGTCGGGGTCAGCATCGGCCTCACCCTGGCGCAACTCATCAGCCTGAACGAGAAGGAT
  GAAGAAATGAGCACAAAGGTGTACTTAGACCTGGAGTGGACCGACTACAGGTTAAGCTGGGACCCCGCAG
  AACACGATGGCATCGAGTCTCTCCGTGTCACTGCTGAATCTGTTTGGCTCCCGGACGTGGTGCTGCTGAA
  CAACAATGATGGAAACTTTGACGTTGCCCTGGACATTAATGTCGTGGTGTCCTTTGAGGGCTCTGTGCGC
  TGGCAACCCCCTGGCCTGTATCGCAGCAGCTGCAGCATCCAGGTCACCTACTTCCCCTTTGACTGGCAGA
  ATTGCACCATGGTGTTTAGTTCCTACAGCTATGACAGCTCTGAGGTCAGCCTGAAGACAGGCCCAGATCC
  TGATGGACAGGAGAGGCAGGAAATCTACATCCATGAAGGGACCTTCATCGAGAATGGTCAATGGGAGATT
  ATCCACAAACCCTCTAGGTTAATCCACCTTCCAGGGGATCGAAGAGGAGGGAAGGAAGGACATCGTGAGG
  AAGTTATCTTCTATCTCATTATTCGGAGGAAGCCTCTCTTCTACCTGGTCAATGTCATTGCCCCGTGCAT
  CCTCATCACCCTCCTGGCCATCTTTGTTTTCTACCTTCCACCAGATGCAGGAGAGAAGATGGGGCTCTCC
  ATCTTTGCCCTGCTGACCCTCACTGTGTTCTTGCTGCTGCTGGCCGACAAAGTGCCTGAGACCTCCCTGG
  CGGTCCCCATCATCATCAAGTATCTCATGTTTACCATGATCCTTGTCACCTTCTCAGTTATCCTTAGCGT
  TGTGGTCCTCAACTTGCACCATCGCTCACCCCACACCCACCAAATGCCCTTTTGGGTCCGCCAGATCTTC
  ATTCACAAGCTCCCTCCATACCTGGGTCTGAAGAGGCCCAAACCCGAGAGAGACCAACTACCCGAACCAC
  ATCACTCCTTCTCCCCAAGGAGTGGCTGGGGCCGAGGAACTGATGAATATTTCATACGGAAGCCTCCATG
  TGATTTTCTTTTCCCTAAACTTAACAGGTTTCAGCCTGAATCACCTGCTCCGGACCTGAGACGATTTATC
  GATGGTCCACCCCGGGCTGTAGGTCTGCCTCAGGAGCTACGCGAGGTCATTTCTTCCATCAGCTACATGG
  CCCGACAACTTCAGGAACAGGAGGACCACGATGCACTGAAGGAGGACTGGCAGTTTGTCGCCATGGTTGT
  GGACCGTCTTTTTCTGTGGACATTCATCGTTTTCACAAGCGTCGGGACGCTAGTCATTTTCCTAGATGCC
  ACGTACCATTTGCCCCCTCCTGAACCTTTTCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR210553 representing NM_012528
  Red=Cloning site Green=Tags(s)
  
  MALGALLLILGILGTPLAPGARGSEAEGQLLKKLFSDYDSSVRPAQEVGDRVGVSIGLTLAQLISLNEKD
  EEMSTKVYLDLEWTDYRLSWDPAEHDGIESLRVTAESVWLPDVVLLNNNDGNFDVALDINVVVSFEGSVR
  WQPPGLYRSSCSIQVTYFPFDWQNCTMVFSSYSYDSSEVSLKTGPDPDGQERQEIYIHEGTFIENGQWEI
  IHKPSRLIHLPGDRRGGKEGHREEVIFYLIIRRKPLFYLVNVIAPCILITLLAIFVFYLPPDAGEKMGLS
  IFALLTLTVFLLLLADKVPETSLAVPIIIKYLMFTMILVTFSVILSVVVLNLHHRSPHTHQMPFWVRQIF
  IHKLPPYLGLKRPKPERDQLPEPHHSFSPRSGWGRGTDEYFIRKPPCDFLFPKLNRFQPESPAPDLRRFI
  DGPPRAVGLPQELREVISSISYMARQLQEQEDHDALKEDWQFVAMVVDRLFLWTFIVFTSVGTLVIFLDA
  TYHLPPPEPFP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012528
• ORF Size: 1503 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_012528.1, NP_036660.1
• RefSeq Size: 2283
• RefSeq ORF: 1506
• Locus ID: 24261
• MW: 57 kDa
• Gene Summary: beta subunit of the nicotinic acetylcholine receptor; can assemble into functionally different acetylcholine receptor channel subtypes [RGD, Feb 2006]