Atp6v1f (NM_053884) Rat Tagged ORF Clone

CAT#: RR210680

Atp6v1f (Myc-DDK-tagged ORF) - Rat ATPase, H transporting, lysosomal V1 subunit F (Atp6v1f), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Atp6v1f
• Synonyms: Atp6s14
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR210680 representing NM_053884
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGGCAGGGGTAAACTAATCGCAGTGATTGGAGACGAGGACACGGTGACTGGTTTCCTGTTGGGCG
  GCATAGGGGAGCTTAACAAGAACCGCCACCCTAATTTCCTGGTAGTGGAGAAGGATACCACCATCAATGA
  AATCGAAGACACTTTCAGGCAGTTTCTAAACAGGGATGACATCGGCATCATTCTCATCAACCAGTACATC
  GCAGAGATGGTTCGGCACGCCCTCGATGCCCACCAGAGGTCCATTCCAGCCGTCCTGGAGATCCCGTCCA
  AGGAGCATCCCTACGACGCAGCCAAAGACTCCATCCTGCGCAGGGCCAAGGGCATGTTCACTGCTGAAGA
  CCTGCGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR210680 representing NM_053884
  Red=Cloning site Green=Tags(s)
  
  MAGRGKLIAVIGDEDTVTGFLLGGIGELNKNRHPNFLVVEKDTTINEIEDTFRQFLNRDDIGIILINQYI
  AEMVRHALDAHQRSIPAVLEIPSKEHPYDAAKDSILRRAKGMFTAEDLR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053884
• ORF Size: 357 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_053884.1, NP_446336.1
• RefSeq Size: 667
• RefSeq ORF: 360
• Locus ID: 116664
• MW: 13.4 kDa
• Gene Summary: Subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. [UniProtKB/Swiss-Prot Function]