Bhmt (NM_030850) Rat Tagged ORF Clone

CAT#: RR210729

Bhmt (Myc-DDK-tagged ORF) - Rat betaine-homocysteine methyltransferase (Bhmt), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Bhmt
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR210729 representing NM_030850
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCACCGATTGCCGGCAAGAAGGCCAAGAGGGGAATCTTAGAACGCTTAAATGCTGGCGAAGTCGTGA
  TCGGAGATGGGGGATTTGTCTTTGCACTGGAAAAGAGGGGCTACGTAAAGGCTGGACCCTGGACCCCAGA
  GGCTGCGGTGGAGCACCCCGAGGCAGTTCGGCAGCTTCATCGGGAGTTCCTCAGAGCTGGATCGAACGTC
  ATGCAGACCTTCACTTTCTATGCAAGTGAGGACAAGCTGGAAAACCGAGGGAACTACGTGGCAGAGAAGA
  TATCTGGGCAGAAGGTCAATGAAGCTGCTTGTGACATTGCACGGCAAGTTGCTGACGAAGGGGATGCATT
  GGTTGCAGGAGGTGTGAGTCAGACACCTTCCTACCTCAGCTGCAAGAGTGAGACGGAAGTTAAAAAGATA
  TTTCACCAACAGCTTGAGGTCTTCATGAAGAAGAATGTGGACTTCCTCATTGCAGAGTATTTTGAACATG
  TTGAAGAAGCCGTGTGGGCAGTCGAGGCCTTAAAAACATCCGGGAAGCCTATAGCGGCTACCATGTGCAT
  CGGACCTGAAGGAGATCTACATGGCGTGTCTCCTGGAGAGTGCGCAGTGCGTTTGGTAAAAGCAGGTGCC
  GCCATTGTCGGTGTGAACTGCCACTTCGACCCCAGCACCAGCTTGCAGACAATAAAGCTCATGAAGGAGG
  GTCTGGAAGCAGCTCGGCTGAAGGCTTACTTGATGAGCCACGCCCTGGCCTACCACACCCCTGACTGTGG
  CAAACAGGGATTTATTGATCTCCCAGAATTCCCCTTTGGATTGGAACCCAGAGTTGCCACCAGATGGGAT
  ATTCAAAAATACGCCAGAGAGGCCTACAACCTGGGGGTCAGGTACATTGGCGGCTGCTGCGGATTTGAGC
  CCTACCACATCAGGGCCATTGCAGAGGAGCTCGCCCCAGAAAGGGGATTTTTACCACCAGCTTCAGAAAA
  ACATGGCAGCTGGGGAAGTGGTTTGGACATGCACACCAAACCCTGGATCAGGGCAAGGGCCAGGAAAGAA
  TACTGGCAGAATCTTCGAATAGCTTCGGGCAGACCGTACAATCCTTCGATGTCCAAGCCGGATGCTTGGG
  GAGTGACGAAAGGGGCAGCAGAGCTGATGCAGCAGAAGGAAGCCACCACTGAGCAGCAGCTGAGAGCGCT
  CTTCGAAAAACAAAAATTCAAATCCGCACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR210729 representing NM_030850
  Red=Cloning site Green=Tags(s)
  
  MAPIAGKKAKRGILERLNAGEVVIGDGGFVFALEKRGYVKAGPWTPEAAVEHPEAVRQLHREFLRAGSNV
  MQTFTFYASEDKLENRGNYVAEKISGQKVNEAACDIARQVADEGDALVAGGVSQTPSYLSCKSETEVKKI
  FHQQLEVFMKKNVDFLIAEYFEHVEEAVWAVEALKTSGKPIAATMCIGPEGDLHGVSPGECAVRLVKAGA
  AIVGVNCHFDPSTSLQTIKLMKEGLEAARLKAYLMSHALAYHTPDCGKQGFIDLPEFPFGLEPRVATRWD
  IQKYAREAYNLGVRYIGGCCGFEPYHIRAIAEELAPERGFLPPASEKHGSWGSGLDMHTKPWIRARARKE
  YWQNLRIASGRPYNPSMSKPDAWGVTKGAAELMQQKEATTEQQLRALFEKQKFKSAQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_030850
• ORF Size: 1221 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_030850.1, NP_110477.1
• RefSeq Size: 1884
• RefSeq ORF: 1224
• Locus ID: 81508
• MW: 45 kDa
• Gene Summary: displays differential expression in a model of liver cirrhosis [RGD, Feb 2006]