Cyp11a1 (NM_017286) Rat Tagged ORF Clone

CAT#: RR210753

Cyp11a1 (Myc-DDK-tagged ORF) - Rat cytochrome P450, family 11, subfamily a, polypeptide 1 (Cyp11a1), nuclear gene encoding mitochondrial protein, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cyp11a1
• Synonyms: Cyp11a; Cypxia1; P450(scc); P450scc
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR210753 representing NM_017286
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGCAAAAGGTCTTTGCCTGCGCTCAGTGCTGGTCAAAAGCTGCCAACCTTTCCTGAGCCCTGTGT
  GGCAGGGTCCAGGGCTGGCCACTGGAAATGGAGCTGGTATCTCCTCTACCAACAGTCCTCGATCCTTCAA
  TGAGATCCCTTCCCCTGGTGACAATGGTTGGATAAACCTGTACCACTTCCTGAGGGAGAACGGCACACAC
  AGAATCCATTATCATCACATGCAGAATTTCCAGAAGTATGGCCCCATTTACAGGGAGAAGCTGGGCAACA
  TGGAGTCAGTTTACATCCTGGACCCTAAGGACGCAGCGACTCTCTTCTCCTGCGAGGGTCCTAACCCGGA
  GCGCTACCTTGTGCCCCCCTGGGTGGCCTATCACCAGTATTACCAGAGGCCCATTGGGGTACTGTTTAAG
  AGTTCAGATGCCTGGAGGAAAGACAGAATTGTCCTAAACCAAGAGGTGATGGCCCCTGACTCCATCAAGA
  ACTTCGTGCCCCTGCTGGAAGGTGTAGCTCAGGACTTCATCAAAGTCTTACACAGACGCATCAAGCAGCA
  AAACTCTGGAAAGTTCTCAGGGGACATCAGCGATGACCTATTCCGCTTTGCCTTTGAGTCCATCACCAGT
  GTTGTATTTGGGGAGCGCCTGGGGATGTTGGAGGAGATCGTGGACCCTGAGTCCCAGCGGTTCATCGACG
  CCGTCTACCAGATGTTCCACACCAGTGTTCCCATGCTCAACATGCCTCCAGACTTATTTCGACTCTTTAG
  AACTAAGACCTGGAAGGACCATGCAGCTGCCTGGGATGTGATTTTCAGTAAAGCTGATGAATACACCCAG
  AACTTCTACTGGGACTTAAGGCAGAAGCGAGACTTCAGCAAGTACCCTGGTGTCCTTTATAGCCTCCTGG
  GAGGCAACAAGCTGCCCTTCAAGAACATCCAGGCCAACATCACAGAGATGCTGGCAGGAGGGGTGGACAC
  GACCTCCATGACTCTGCAATGGAACCTTTATGAAATGGCACACAACTTGAAGGTACAGGAGATGCTGCGG
  GCTGAAGTTCTAGCTGCCCGGCGCCAGGCCCAGGGAGACATGGCCAAGATGGTCCAGTTGGTCCCACTCC
  TCAAAGCCAGCATCAAGGAGACACTGAGACTCCACCCCATCTCTGTGACCTTGCAGAGGTATATTGTGAA
  CGACCTGGTGCTTCGTAATTACAAGATTCCAGCCAAGACTTTGGTGCAGGTGGCTAGCTATGCCATGGGT
  CGAGAATCCAGCTTCTTTCCCAATCCAAACAAGTTTGACCCAACTCGTTGGTTGGAAAAAAGCCAAAACA
  CCACGCACTTCCGGTACTTGGGCTTTGGCTGGGGTGTTCGGCAGTGTCTGGGCCGGCGGATTGCAGAGCT
  GGAAATGACCATCTTCCTCATCAACGTGCTGGAAAACTTCAGAATTGAAGTTCAAAGTATCCGTGATGTG
  GGGACCAAGTTCAACCTCATCCTGATGCCTGAGAAGCCTATCTTCTTCAACTTCCAGCCTCTCAAGCAGG
  ACCTGGGCTCAACTATGCCCAGAAAAGGCGACACTGTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR210753 representing NM_017286
  Red=Cloning site Green=Tags(s)
  
  MLAKGLCLRSVLVKSCQPFLSPVWQGPGLATGNGAGISSTNSPRSFNEIPSPGDNGWINLYHFLRENGTH
  RIHYHHMQNFQKYGPIYREKLGNMESVYILDPKDAATLFSCEGPNPERYLVPPWVAYHQYYQRPIGVLFK
  SSDAWRKDRIVLNQEVMAPDSIKNFVPLLEGVAQDFIKVLHRRIKQQNSGKFSGDISDDLFRFAFESITS
  VVFGERLGMLEEIVDPESQRFIDAVYQMFHTSVPMLNMPPDLFRLFRTKTWKDHAAAWDVIFSKADEYTQ
  NFYWDLRQKRDFSKYPGVLYSLLGGNKLPFKNIQANITEMLAGGVDTTSMTLQWNLYEMAHNLKVQEMLR
  AEVLAARRQAQGDMAKMVQLVPLLKASIKETLRLHPISVTLQRYIVNDLVLRNYKIPAKTLVQVASYAMG
  RESSFFPNPNKFDPTRWLEKSQNTTHFRYLGFGWGVRQCLGRRIAELEMTIFLINVLENFRIEVQSIRDV
  GTKFNLILMPEKPIFFNFQPLKQDLGSTMPRKGDTV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017286
• ORF Size: 1578 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_017286.1, NM_017286.2, NM_017286.3, NP_058982.1
• RefSeq Size: 1581
• RefSeq ORF: 1581
• Locus ID: 29680
• MW: 60.6 kDa
• Gene Summary: monooxygenase that catalyzes synthesis of cholesterol and steroids [RGD, Feb 2006]