Asmt (NM_144759) Rat Tagged ORF Clone

CAT#: RR210836

Asmt (Myc-DDK-tagged ORF) - Rat acetylserotonin O-methyltransferase (Asmt), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Asmt
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR210836 representing NM_144759
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCACCCGGCCGGGAGGGCGAGCTTGACCGCGACTTCCGAGTCCTTATGAGCCTCGCCCACGGCTTCA
  TGGTCTCCCAGGTGCTGTTTGCTGCCCTGGATCTGGGCATCTTCGACCTTGCGGCCCAGGGCCCGGTGGC
  AGCGGAGGCGGTGGCACAGACTGGGGGGTGGAGTCCGCGGGGAACGCAGCTGCTAATGGACGCCTGCACC
  AGGCTGGGGCTACTAAGAGGGGCCGGGGACGGCTCCTACACCAACTCTGCGCTGTCATCCACATTCCTGG
  TGAGCGGAAGCCCCCAGTCTCAACGCTGCATGCTGCTCTACCTGGCGGGCACAACATACGGCTGCTGGGC
  CCACCTGGCTGCTGGGGTCAGGGAAGGGCGGAACCAGTACTCCAGGGCCGTGGGCATCTCCGCTGAGGAC
  CCCTTTTCGGCCATCTACAGGTCAGAGCCTGAGCGCCTGCTGTTCATGAGGGGCCTGCAGGAGACATGGA
  GTCTATGCGGGGGACGGGTCCTCACAGCCTTTGACCTCTCACGCTTCCGGGTCATCTGTGACCTTGGCGG
  CGGGTCTGGGGCGCTGGCACAGGAGGCCGCCCGCCTCTATCCCGGTAGCTCCGTGTGTGTCTTTGATCTC
  CCTGATGTCATCGCCGCTGCCCGCACCCACTTCCTGTCGCCAGGGGCAAGACCCAGTGTGAGGTTTGTCG
  CTGGTGACTTCTTCCGTTCCCGCCTCCCCCGCGCTGACCTCTTCATTCTTGCCCGGGTCCTGCACGACTG
  GGCTGATGGTGCCTGCGTGGAGTTGCTGGGGCGGCTGCACAGGGCCTGCAGGCCAGGTGGTGCACTACTG
  CTGGTGGAGGCAGTGCTGGCCAAGGGCGGGGCTGGGCCGCTGCGGTCGCTCCTGCTGTCACTCAACATGA
  TGCTGCAGGCTGAGGGGTGGGAGCGCCAGGCCAGCGACTACCGCAACCTGGCCACTCGTGCAGGCTTCCC
  CCGCCTGCAGCTGCGGCGTCCTGGTGGGCCATACCATGCCATGCTGGCCCGGCGGGGACCCCGCCCTGGG
  ATCATCACAGGAGTGGGAAGTAACACTACGGGGACAGGAAGTTTTGTCACAGGTATAAGACGTGATGTCC
  CTGGGGCAAGAAGTGACGCTGCAGGGACAGGAAGTGGCACTGGAAACACAGGAAGTGGCATCATGCTACA
  GGGAGAGACGTTGGAATCAGAGGTCAGCGCCCCACAAGCAGGAAGTGACGTTGGCGGGGCAGGAAATGAA
  CCCAGAAGTGGCACCCTCAAGCAAGGAGACTGGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR210836 representing NM_144759
  Red=Cloning site Green=Tags(s)
  
  MAPGREGELDRDFRVLMSLAHGFMVSQVLFAALDLGIFDLAAQGPVAAEAVAQTGGWSPRGTQLLMDACT
  RLGLLRGAGDGSYTNSALSSTFLVSGSPQSQRCMLLYLAGTTYGCWAHLAAGVREGRNQYSRAVGISAED
  PFSAIYRSEPERLLFMRGLQETWSLCGGRVLTAFDLSRFRVICDLGGGSGALAQEAARLYPGSSVCVFDL
  PDVIAAARTHFLSPGARPSVRFVAGDFFRSRLPRADLFILARVLHDWADGACVELLGRLHRACRPGGALL
  LVEAVLAKGGAGPLRSLLLSLNMMLQAEGWERQASDYRNLATRAGFPRLQLRRPGGPYHAMLARRGPRPG
  IITGVGSNTTGTGSFVTGIRRDVPGARSDAAGTGSGTGNTGSGIMLQGETLESEVSAPQAGSDVGGAGNE
  PRSGTLKQGDWK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144759
• ORF Size: 1296 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_144759.2, NP_653360.2
• RefSeq Size: 1548
• RefSeq ORF: 1299
• Locus ID: 246281
• MW: 45.6 kDa
• Gene Summary: methyltransferase enzyme; catalyzes the methylation of acetylserotonin to complete the synthesis of melatonin [RGD, Feb 2006]