Pgk1 (NM_053291) Rat Tagged ORF Clone

CAT#: RR210973

Pgk1 (Myc-DDK-tagged ORF) - Rat phosphoglycerate kinase 1 (Pgk1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pgk1
• Synonyms: Pgk
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR210973 representing NM_053291
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGCTTTCTAACAAGCTGACTTTGGACAAGCTGGACGTGAAGGGGAAGCGGGTCGTGATGAGGGTGG
  ACTTCAATGTTCCTATGAAGAACAACCAGATAACGAATAACCAAAGGATCAAGGCTGCTGTCCCAAGCAT
  CAAATTCTGCTTGGACAATGGAGCCAAGTCGGTTGTGCTTATGAGCCACCTGGGCCGTCCTGATGGTGTG
  CCCATGCCCGACAAGTACTCCTTAGAGCCAGTTGCTGCAGAACTCAAATCTCTGCTGGGCAAGGATGTTC
  TGTTCTTGAAGGATTGTGTGGGCTCAGAAGTAGAGAATGCCTGTGCCAACCCAGCGGCTGGGACTGTCAT
  CCTCCTGGAGAACCTCCGCTTTCATGTAGAGGAAGAAGGGAAGGGAAAAGATGCTTCTGGGAACAAGGTT
  AAAGCTGAGCCAGCTAAAATTGATGCTTTCCGAGCCTCCCTGTCCAAACTTGGAGATGTCTATGTCAATG
  ATGCTTTTGGGACTGCACACAGAGCCCACAGTTCCATGGTGGGTGTGAATCTGCCACAGAAGGCTGGTGG
  ATTTTTGATGAAGAAGGAGCTGAACTACTTTGCCAAGGCTTTGGAGAGTCCAGAGCGACCCTTCCTGGCT
  ATCTTGGGAGGAGCTAAAGTTGCAGACAAGATCCAGCTGATCAATAATATGCTAGACAAAGTCAATGAGA
  TGATCATCGGTGGGGGAATGGCTTTTACCTTCCTTAAGGTGCTCAACAACATGGAGATTGGCACATCTCT
  GTATGATGAAGAGGGAGCCAAGATTGTCAAAGATCTCATGGCCAAAGCTGAGAAAAATGGTGTGAAGATT
  ACCTTGCCTGTTGACTTTGTCACTGCTGACAAATTTGATGAGAATGCAAAGACTGGCCAAGCTACTGTGG
  CCTCTGGTATACCTGCTGGCTGGATGGGCTTGGACTGTGGTACTGAGAGCAGTAAGAAATATGCTGAGGC
  TGTGGCTCGAGCTAAGCAGATTGTTTGGAACGGTCCTGTTGGGGTATTTGAATGGGAAGCCTTTGCCAGG
  GGAACCAAGTCCCTCATGGATGAGGTGGTGAAAGCCACGTCTAGGGGCTGCATCACTATCATAGGAGGCG
  GAGACACCGCCACTTGCTGTGCCAAATGGAACACAGAGGATAAAGTCAGCCATGTGAGCACTGGGGGCGG
  CGCCAGTCTAGAGCTCCTGGAAGGTAAAGTCCTTCCTGGGGTGGATGCTCTCAGCAATGTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR210973 representing NM_053291
  Red=Cloning site Green=Tags(s)
  
  MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAVPSIKFCLDNGAKSVVLMSHLGRPDGV
  PMPDKYSLEPVAAELKSLLGKDVLFLKDCVGSEVENACANPAAGTVILLENLRFHVEEEGKGKDASGNKV
  KAEPAKIDAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVNLPQKAGGFLMKKELNYFAKALESPERPFLA
  ILGGAKVADKIQLINNMLDKVNEMIIGGGMAFTFLKVLNNMEIGTSLYDEEGAKIVKDLMAKAEKNGVKI
  TLPVDFVTADKFDENAKTGQATVASGIPAGWMGLDCGTESSKKYAEAVARAKQIVWNGPVGVFEWEAFAR
  GTKSLMDEVVKATSRGCITIIGGGDTATCCAKWNTEDKVSHVSTGGGASLELLEGKVLPGVDALSNV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053291
• ORF Size: 1251 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_053291.1, NM_053291.2, NM_053291.3, NP_445743.2
• RefSeq Size: 1685
• RefSeq ORF: 1254
• Locus ID: 24644
• MW: 44.5 kDa
• Gene Summary: kinase enzyme that is important for phosphoprotein glycolysis [RGD, Feb 2006]