Map3k11 (NM_001013150) Rat Tagged ORF Clone

CAT#: RR210995

Map3k11 (Myc-DDK-tagged ORF) - Rat mitogen-activated protein kinase kinase kinase 11 (Map3k11), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Map3k11
• Synonyms: Mlk-3; Mlk3; RHOE
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR210995 representing NM_001013150
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCCTTGAAGAACCTCTTCCTCAAGAGCCCGCTGGGGTCATGGAACGGCAGTGGCAGTGGGGGCG
  GTGGGGGTTCTGGTGGAGTCCGGCCAGAGGGGTCCCCGAAGGCAACAGCAGCTTATGCCAACCCTGTCTG
  GACAGCCCTGTTCGACTATGAGCCCAATGGGCAGGACGAACTAGCCCTGAGGAAGGGCGACCGTGTGGAG
  GTGCTGTCCAGGGATGCAGCCATCTCAGGAGATGAAGGCTGGTGGGCGGGCCAGGTGGGTGGCCAGGTGG
  GCATTTTTCCATCCAACTATGTATCTCGGGGTGGAGGCCCACCCCCCTGTGAGGTGGCCAGCTTCCAGGA
  GCTGCGACTGGAGGAGGTGATCGGCATCGGTGGCTTCGGCAAGGTCTACCGTGGCAGCTGGCGAGGGGAG
  CTGGTGGCTGTGAAAGCAGCTCGACAGGATCCTGATGAGGACATCAGTGTAACAGCTGAGAGTGTTCGAC
  AGGAGGCCCGGCTTTTTGCCATGCTGGCACATCCCAACATCATTGCCCTCAAAGCTGTGTGCCTGGAGGA
  GCCAAACCTATGCCTGGTGATGGAGTATGCAGCTGGTGGACCTCTTAGCCGTGCCCTGGCTGGTCGGCGG
  GTGCCCCCCCATGTGCTGGTCAACTGGGCTGTGCAGATTGCCCGAGGGATGCACTACCTGCACTGTGAGG
  CTCTAGTGCCTGTGATACACCGAGACCTGAAGTCCAACAACATTCTGCTGCTGCAGCCCATTGAAGGTGA
  CGACATGGAGCACAAGACCCTAAAGATTACTGACTTTGGCCTCGCCCGAGAGTGGCACAAAACCACACAG
  ATGAGTGCTGCGGGGACCTATGCGTGGATGGCTCCTGAGGTTATCAAGGCATCTACCTTCTCCAAGGGCA
  GCGATGTCTGGAGCTTTGGGGTGCTGCTGTGGGAACTGCTGACTGGGGAGGTACCCTACCGTGGTATCGA
  CTGTCTTGCGGTAGCCTATGGTGTGGCTGTAAACAAGTTAACATTACCCATCCCATCCACTTGCCCTGAG
  CCCTTCGCACAACTCATGGCTGACTGCTGGGCACAGGACCCCCACCGCAGGCCCGACTTCGCCTCCATCC
  TGCAGCAGTTGGAAGCTCTAGAGGCTCAGGTGCTGAGGGAGATGCCACGGGACTCCTTCCATTCCATGCA
  GGAGGGCTGGAAACGCGAGATCCAGGGCCTTTTTGATGAGCTGCGGGCCAAGGAAAAGGAACTACTGAGC
  CGCGAGGAGGAACTGACGCGCGCAGCACGCGAGCAGCGGTCCCAGGCAGAGCAGCTGCGACGGCGCGAGC
  ACCTGCTGGCGCAGTGGGAGCTGGAGGTGTTCGAGCGCGAGCTGACACTGCTGCTACAGCAGGTGGACCG
  AGAACGGCCACACGTGCGCCGCCGACGCGGCACCTTCAAGCGGAGCAAGCTCCGAGCGAGGGATGGCGGA
  GAACGCATCAGCATGCCCCTGGACTTCAAACATCGCATCACTGTGCAGGCCTCTCCAGGACTTGACCGGA
  GGAGAAATGTGTTCGAGGTTGGGGCTGGGGACTCGCCCACCTTCCCTCGGTTCCGAGCTATCCAGTTGGA
  ACCTGCAGAATCAGGCCAGACTTGGGGTCGCCAGTCCCCTAGACGTCTTGACGATTCAAGCAATGGAGAG
  AGGCGTGCTTGCTGGGCCTGGGGTCCCAGTTCCCCCAAGCCTGGGGAGGCCCAGAATGGGAGGAGAAGGT
  CCCGCATGGATGAAGCCACGTGGTACCTAGATTCAGATGACTCATCCCCATTAGGGTCTCCTTCTACTCC
  CCCAGCACTCAACGGTAATCCTCCAAGGCCTAGCCCTGAGCCAGAGGAGCCACGGCGGTCTGGGCCCACA
  GAACGGGGCAATAGCTCTGGGACGCCTAAACTGATCCAACGTGCATTGCTCCGGGGGACCGCTCTGCTGG
  CTTCCCTAGGCCTTGGCCGAGACCTGCAGCCTCCAGGAGGCCTGAGCCGTGAACGTGGAGAGTCCCCGAC
  AGCACCACCCCCTGCACAGATGGCTTCACCCTGTCCACCTGACCTGCCCTCCACCCCGCTCATCCACCTC
  TCACAAGCAACACCTGATGCCCGTGGACCACTCACCCCTGCACCCTTATTGCTGGACCTAGGTGTTTCCT
  CTGGCCAGCCATCAGCCAAGAGCCCTCGGCGGGAAGAGACACGTGGAAGAACTGTTTCACCTCCACCAGG
  AATATCACGCTCTGCTCCTGGCACCCCTGGAACCCCTCGCTCACCACCTTTGGGCCTCATCAGCCGACCT
  CGGCCATCACCACTCCGTAACCGTATTGACCCATGGAGCTTCGTGTCAGCTGGGCCACGGCCTTCACCCC
  TGCCCTCTCCACAGCCTGCACCCCGACGGGCACCATGGACCTTATTCCCAGACTCAGATCCCTTCTGGGA
  CTCCCCACCTGCCAACCCCTTCCGGGGAGGCTCCCAGGACTGCAGGACGCAGACCAAAGATGTGGGTGCT
  CAGGCGCCATGGGCACCAGAAGCAGGGCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR210995 representing NM_001013150
  Red=Cloning site Green=Tags(s)
  
  MEPLKNLFLKSPLGSWNGSGSGGGGGSGGVRPEGSPKATAAYANPVWTALFDYEPNGQDELALRKGDRVE
  VLSRDAAISGDEGWWAGQVGGQVGIFPSNYVSRGGGPPPCEVASFQELRLEEVIGIGGFGKVYRGSWRGE
  LVAVKAARQDPDEDISVTAESVRQEARLFAMLAHPNIIALKAVCLEEPNLCLVMEYAAGGPLSRALAGRR
  VPPHVLVNWAVQIARGMHYLHCEALVPVIHRDLKSNNILLLQPIEGDDMEHKTLKITDFGLAREWHKTTQ
  MSAAGTYAWMAPEVIKASTFSKGSDVWSFGVLLWELLTGEVPYRGIDCLAVAYGVAVNKLTLPIPSTCPE
  PFAQLMADCWAQDPHRRPDFASILQQLEALEAQVLREMPRDSFHSMQEGWKREIQGLFDELRAKEKELLS
  REEELTRAAREQRSQAEQLRRREHLLAQWELEVFERELTLLLQQVDRERPHVRRRRGTFKRSKLRARDGG
  ERISMPLDFKHRITVQASPGLDRRRNVFEVGAGDSPTFPRFRAIQLEPAESGQTWGRQSPRRLDDSSNGE
  RRACWAWGPSSPKPGEAQNGRRRSRMDEATWYLDSDDSSPLGSPSTPPALNGNPPRPSPEPEEPRRSGPT
  ERGNSSGTPKLIQRALLRGTALLASLGLGRDLQPPGGLSRERGESPTAPPPAQMASPCPPDLPSTPLIHL
  SQATPDARGPLTPAPLLLDLGVSSGQPSAKSPRREETRGRTVSPPPGISRSAPGTPGTPRSPPLGLISRP
  RPSPLRNRIDPWSFVSAGPRPSPLPSPQPAPRRAPWTLFPDSDPFWDSPPANPFRGGSQDCRTQTKDVGA
  QAPWAPEAGP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001013150
• ORF Size: 2550 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001013150.1, NP_001013168.1
• RefSeq Size: 3536 bp
• RefSeq ORF: 2553 bp
• Locus ID: 309168
• Cytogenetics: 1q43
• MW: 93.1 kDa