Prim2 (NM_001024762) Rat Tagged ORF Clone

CAT#: RR211010

Prim2 (Myc-DDK-tagged ORF) - Rat primase, DNA, polypeptide 2 (Prim2), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Prim2
• Synonyms: Pola3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR211010 representing NM_001024762
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGTTCTCAGGAAGGACCCGGAAGAAGCTGAGATTGGCAGGTGACCAGAGAAACGCTTGTTACCCTC
  ACAGCCTTCAGTTCTATCTGCAGCCACCTACTGAAAACATATCATTGACAGAGTTTGAAAGCTTGGCTTT
  TGATAGAGTAAAATTGCTTAAAGCAATTGAGAATCTTGGTGTGAGCTATGTGAAAGGAACCGAACAGTAC
  CAGAGTAAACTGGAGGCTGAGATTCGAAAGCTCAAGTTTTCGTACAGGGAGAACCTGGAGGATGAGTACG
  AGCCTCGGAGGAGGGACCACATCTCCCACTTCATCCTGCGCCTCGCTTACTGCCAGTCGGAAGATCTTAG
  ACGGTGGTTTATTCAACAGGAGATGGATCTGCTTCGGTTCCGATTCAGTATTTTACCCAAGGATAAAGTC
  CAGAGTTTCTTGAAGGATACTCACTTGCATTTTGAGGCTATCAGTGATGAGGAGAAGACCCTTCGGGAAC
  AGGATATCATGGCGTCCTCTCCCAGCCTAAGTGGGGTCAGGTGGGAATCGGAGTCAGTGTATAAGGTCCC
  CTTTGCTGACGCTCTGGACCTGTTCAGAGGAAGGAAAGTCTACTTGGAAGACGGCTTTGCTTATGTGCCA
  CTTAAGGACATTGTGGCCATTATCCTGAACGAGTTTAGAGCCACGCTGTCTAAGGCCTTGGCACTAACAG
  CCAGGTCCCTGCCTGCTGTGCAGTCCGATGAACGACTTCAGCCTCTGCTCAGCCACCTCAGTCATTCTTA
  CACCGGCCAAGATTATAGTACCCAGAAGAGCACCGGGAAGATTTCCTTAGATCAGATTGATTCGCTTTCA
  ACAAAATCCTTCCCACCTTGCATGCGTCAGCTGCACAAGGCGCTGAGGGAAAACCACCATCTTCGTCATG
  GAGGCCGGATGCAGTATGGCCTGTTCCTCAAGGGCATTGGGCTAACGTTGGAGCAAGCATTGCAGTTCTG
  GAAGCAAGAGTTTATCAAAGGAAAGATGGACCCAGACAAGTTTGATAAAGGTTACTCTTACAATATCCGA
  CATAGCTTTGGAAAGGAAGGCAAGAGGACAGACTATACGCCATTCAGTTGCATGAAGATTATCCTGACCA
  ACCCACCAAGCCAGGGGGATTTCCATGGGTGCCCATTCCGTCACAGTGATGCAGAGCTGCTGAAGCAGAA
  GATGCAGACCTACAAGATCCCTGCCTCGGGGATCAGCCAGGTGGACGATTGTGGCTTTTCTTTGAATCAT
  CCGAATCAGTTCTTTTTTGAGAGCCAGCGAATCCTAACTGGTGGCAAAGATATCAAGAAGGAAGCAAGCC
  ACCCAGAAACGCCTCAGCACAAACCCAGCACCCAGAAGACCAAGGATGCCACGTCTGCTCTGGCCTCTCT
  AGATTCCTCCCTGGAAATGGATCTGGAGGGGCTAGAAGACTACTTTAGTAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR211010 representing NM_001024762
  Red=Cloning site Green=Tags(s)
  
  MQFSGRTRKKLRLAGDQRNACYPHSLQFYLQPPTENISLTEFESLAFDRVKLLKAIENLGVSYVKGTEQY
  QSKLEAEIRKLKFSYRENLEDEYEPRRRDHISHFILRLAYCQSEDLRRWFIQQEMDLLRFRFSILPKDKV
  QSFLKDTHLHFEAISDEEKTLREQDIMASSPSLSGVRWESESVYKVPFADALDLFRGRKVYLEDGFAYVP
  LKDIVAIILNEFRATLSKALALTARSLPAVQSDERLQPLLSHLSHSYTGQDYSTQKSTGKISLDQIDSLS
  TKSFPPCMRQLHKALRENHHLRHGGRMQYGLFLKGIGLTLEQALQFWKQEFIKGKMDPDKFDKGYSYNIR
  HSFGKEGKRTDYTPFSCMKIILTNPPSQGDFHGCPFRHSDAELLKQKMQTYKIPASGISQVDDCGFSLNH
  PNQFFFESQRILTGGKDIKKEASHPETPQHKPSTQKTKDATSALASLDSSLEMDLEGLEDYFSK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001024762
• ORF Size: 1452 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001024762.1, NP_001019933.1
• RefSeq Size: 2141 bp
• RefSeq ORF: 1455 bp
• Locus ID: 301323
• Cytogenetics: 9q21
• MW: 55.9 kDa
• Gene Summary: may play a role in DNA replication; V288M mutation is detected in a hepatoma cell line [RGD, Feb 2006]