Ece1 (NM_053596) Rat Tagged ORF Clone

CAT#: RR211166

Ece1 (Myc-DDK-tagged ORF) - Rat endothelin converting enzyme 1 (Ece1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ece1
• Synonyms: Ece
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR211166 representing NM_053596
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGTCATCCTACAAGCGGGCCACGCTGGACGAAGAGGATCTGGTGGACTCACTCTCCGAGGGCGATG
  TGTACCCCAATGGCCTACAGGTGAACTTCCGCAGCCCCCGGAGCGGACAGAGGTGCTGGGCAGCTCGGAC
  CTCGGTGGAGAAGCGGCTGGTGGTTCTGGTGACGCTTCTGGCAGCAGGGCTGGTGGCCTGCCTGGCAGCC
  CTAGGCATCCAGTACCGGACAAGAACGCCTCCGGTATGTCTGACTGAGGCCTGTGTCTCAGTGACCAGCT
  CCATCCTAAACTCCATGGACCCCACGGTAGACCCCTGCCAGGACTTCTTCAGCTACGCCTGTGGTGGCTG
  GATCAAGGCCAACCCCGTTCCCGACGGTCACTCACGCTGGGGGACCTTCAGCAACCTCTGGGAGCACAAC
  CAAGCCATCATTAAGCATCTGCTGGAAAATTCCACGGCCAGCGCGAGCGAGGCAGAGAAAAAGGCGCAAG
  TGTACTACCGTGCGTGTATGAACGAAACTAGGATCGAGGAGCTTCGGGCCAAGCCCCTGATGGAGCTGAT
  TGAGAAGCTCGGAGGTTGGAATATCACAGGACCCTGGGCCAAGGACAACTTCCAGGACACGCTGCAGGTG
  GTCACAGCGCACTACCGCACCTCACCCTTCTTCTCTGTCTATGTCAGTGCCGACTCCAAGAACTCCAACA
  GCAATGTGATCCAGGTGGACCAGTCCGGCCTTGGCTTGCCCTCCAGAGACTATTACCTGAACAAGACGGA
  AAATGAAAAGGTACTGACTGGCTATCTGAACTACATGGTCCAGCTGGGGAAACTGCTGGGTGGTGGGGAC
  GAGGACTCCATCCGGCCCCAGATGCAGCAGATCCTGGATTTTGAGACCGCTCTGGCCAACATCACCATCC
  CCCAGGAGAAGCGCCGGGATGAAGAGCTCATCTACCACAAAGTCACGGCTGCTGAGCTGCAGACCTTGGC
  ACCCGCCATCAACTGGTTACCCTTTCTGAATGCCATTTTTTACCCAGTGGAGATCAATGAGTCTGAGCCC
  ATCGTGGTCTACGACAAGGAATACCTCAGACAAGTCTCCACACTCATCAACAGCACCGACAAATGCCTGC
  TCAACAACTACATGATGTGGAACCTGGTACGGAAAACAAGCTCCTTTCTCGACCAGCGCTTTCAGGATGC
  CGATGAGAAGTTCATGGAGGTTATGTACGGGACAAAGAAGACCTGTCTTCCCCGCTGGAAGTTTTGCGTG
  AGTGACACAGAAAACAACCTGGGCTTTGCCCTGGGCCCCATGTTTGTGAAAGCAACCTTTGCGGAGGACA
  GCAAGAACATAGCCAGCGAGATCATCCTGGAGATCAAGAAGGCATTCGAGGAGAGCCTGAGCACCCTGAA
  ATGGATGGATGAAGATACTCGGAGGTCAGCCAAGGAGAAGGCGGACGCCATCTACAACATGATAGGCTAC
  CCCAACTTCATCATGGACCCCAAGGAGCTGGACAAAGTGTTCAATGACTACACAGCAGTTCCCGATCTCT
  ACTTTGAGAACGCCATGCGATTTTTCAACTTCTCATGGAGGGTCACAGCCGACCAGCTCAGGAAAGCCCC
  CAACAGAGATCAGTGGAGTATGACCCCGCCCATGGTGAACGCCTACTACTCGCCCACCAAGAACGAGATT
  GTGTTTCCAGCTGGAATCCTGCAGGCGCCATTTTATACCCGCTCTTCGCCCAACGCCTTGAACTTTGGTG
  GTATCGGGGTCGTTGTGGGCCACGAGCTGACTCATGCTTTCGACGATCAAGGCCGGGAGTATGACAAGGA
  TGGGAACCTCCGGCCCTGGTGGAAGAACTCGTCGGTGGAGGCATTCAAGCAGCAGACCGAGTGCATGGTA
  CAGCAGTATAACAACTACAGTGTGAACGGAGAGCCCGTGAATGGGCGGCACACCCTCGGGGAGAACATCG
  CGGACAACGGGGGACTCAAGGCAGCCTACCGGGCGTACCAGAACTGGGTAAAGAAGAACGGAGCTGAGCA
  GATACTGCCCACCCTGGGTCTCACCAGCAACCAGCTCTTCTTCCTGGGATTCGCACAGGTCTGGTGCTCG
  GTCCGCACACCAGAGAGCTCCCACGAAGGCCTCATCACCGATCCGCACAGCCCCTCCCGCTTCCGGGTCA
  TCGGCTCACTCTCCAACTCCAAGGAGTTCTCAGAACACTTCCGCTGCCCGCTCGGCTCCCCCATGAACCC
  TCGCCACAAATGCGAAGTCTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR211166 representing NM_053596
  Red=Cloning site Green=Tags(s)
  
  MMSSYKRATLDEEDLVDSLSEGDVYPNGLQVNFRSPRSGQRCWAARTSVEKRLVVLVTLLAAGLVACLAA
  LGIQYRTRTPPVCLTEACVSVTSSILNSMDPTVDPCQDFFSYACGGWIKANPVPDGHSRWGTFSNLWEHN
  QAIIKHLLENSTASASEAEKKAQVYYRACMNETRIEELRAKPLMELIEKLGGWNITGPWAKDNFQDTLQV
  VTAHYRTSPFFSVYVSADSKNSNSNVIQVDQSGLGLPSRDYYLNKTENEKVLTGYLNYMVQLGKLLGGGD
  EDSIRPQMQQILDFETALANITIPQEKRRDEELIYHKVTAAELQTLAPAINWLPFLNAIFYPVEINESEP
  IVVYDKEYLRQVSTLINSTDKCLLNNYMMWNLVRKTSSFLDQRFQDADEKFMEVMYGTKKTCLPRWKFCV
  SDTENNLGFALGPMFVKATFAEDSKNIASEIILEIKKAFEESLSTLKWMDEDTRRSAKEKADAIYNMIGY
  PNFIMDPKELDKVFNDYTAVPDLYFENAMRFFNFSWRVTADQLRKAPNRDQWSMTPPMVNAYYSPTKNEI
  VFPAGILQAPFYTRSSPNALNFGGIGVVVGHELTHAFDDQGREYDKDGNLRPWWKNSSVEAFKQQTECMV
  QQYNNYSVNGEPVNGRHTLGENIADNGGLKAAYRAYQNWVKKNGAEQILPTLGLTSNQLFFLGFAQVWCS
  VRTPESSHEGLITDPHSPSRFRVIGSLSNSKEFSEHFRCPLGSPMNPRHKCEVW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053596
• ORF Size: 2262 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_053596.1, NM_053596.2, NP_446048.2
• RefSeq Size: 2808
• RefSeq ORF: 2265
• Locus ID: 94204
• MW: 85.6 kDa
• Gene Summary: catalyzes the conversion of the big endothelin precursor (big ET-1) to the vasoconstrictive peptide endothelin [RGD, Feb 2006]