Cyp24a1 (NM_201635) Rat Tagged ORF Clone

CAT#: RR211209

Cyp24a1 (Myc-DDK-tagged ORF) - Rat cytochrome P450, subfamily 24 (Cyp24a1), nuclear gene encoding mitochondrial protein, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cyp24a1
• Synonyms: 24-OHase; 24R-Ohase; Cyp24; P450-CC24; VITD12
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR211209 representing NM_201635
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTGCCCCATTGACAAACGGCGCACCCTGATCGCTTTTCTGCGCCGACTGCGCGACCTGGGACAGC
  CCCCAAGGTCGGTGACATCCAAGGCGAGCGCTTCCCGCGCTCCAAAAGAGGTGCCCCTCTGCCCGCTGAT
  GACAGACGGTGAGACTCGAAACGTCACCTCCTTGCCTGGGCCCACCAACTGGCCACTGCTAGGGAGTCTA
  CTGGAGATTTTTTGGAAAGGTGGCCTGAAGAAACAGCACGACACGCTGGCAGAGTACCACAAGAAGTATG
  GCCAGATTTTCCGGATGAAGCTGGGCTCCTTCGACTCCGTGCATCTGGGCTCGCCGAGCCTGCTGGAAGC
  TCTGTACCGCACAGAGAGCGCGCATCCCCAGCGACTGGAGATCAAACCTTGGAAAGCCTATCGCGACCAC
  AGGAACGAAGCCTACGGGTTGATGATCCTGGAAGGACAGGAGTGGCAAAGGGTCCGCAGCGCCTTCCAGA
  AGAAACTCATGAAGCCCGTGGAGATCATGAAGCTGGACAAGAAAATCAATGAGGTCTTGGCTGACTTTCT
  AGAGCGCATGGATGAGCTGTGCGATGAGCGAGGCCGCATCCCAGACCTGTACAGTGAGCTGAACAAGTGG
  TCCTTTGAAAGCATCTGCCTTGTGTTATATGAGAAGAGATTTGGGCTCCTTCAAAAGGAGACAGAAGAAG
  AAGCGCTGACCTTCATTACGGCCATCAAAACGATGATGAGCACGTTTGGGAAGATGATGGTGACCCCCGT
  GGAGTTGCACAAGCGCCTCAACACCAAAGTGTGGCAGGCACATACGCTGGCTTGGGACACCATTTTCAAA
  TCAGTCAAACCCTGCATCGACAACCGCCTACAGAGATATTCCCAGCAGCCCGGGGCAGATTTCCTCTGTG
  ATATTTATCAGCAAGATCACCTTTCCAAGAAGGAACTGTACGCCGCTGTCACGGAGCTGCAGCTCGCTGC
  AGTGGAGACGACCGCGAACAGCTTGATGTGGATTCTCTACAATCTATCCCGGAATCCTCAAGCGCAACGG
  AGACTCCTTCAGGAAGTTCAGAGCGTGCTGCCTGACAATCAGACGCCACGGGCGGAAGACCTGAGGAATA
  TGCCCTATTTAAAGGCCTGTCTGAAAGAATCCATGAGGCTTACCCCAAGTGTGCCATTTACAACTCGGAC
  CCTTGACAAACCAACGGTTCTGGGTGAATACGCTCTACCCAAAGGAACAGTGTTAACCCTCAATACCCAA
  GTGCTGGGTTCTAGTGAAGACAATTTTGAAGATTCTCATAAGTTCAGACCCGAACGCTGGCTTCAAAAGG
  AGAAAAAGATCAACCCCTTCGCTCATCTCCCATTCGGCATCGGGAAGAGAATGTGCATCGGGCGCCGGCT
  GGCTGAGTTACAGCTCCACCTGGCTCTCTGCTGGATAATCCAAAAATACGACATTGTGGCCACAGACAAT
  GAACCTGTGGAGATGCTGCACCTTGGCATCCTGGTACCCAGCCGGGAGCTGCCAATTGCGTTCCGTCCAC
  GG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR211209 representing NM_201635
  Red=Cloning site Green=Tags(s)
  
  MSCPIDKRRTLIAFLRRLRDLGQPPRSVTSKASASRAPKEVPLCPLMTDGETRNVTSLPGPTNWPLLGSL
  LEIFWKGGLKKQHDTLAEYHKKYGQIFRMKLGSFDSVHLGSPSLLEALYRTESAHPQRLEIKPWKAYRDH
  RNEAYGLMILEGQEWQRVRSAFQKKLMKPVEIMKLDKKINEVLADFLERMDELCDERGRIPDLYSELNKW
  SFESICLVLYEKRFGLLQKETEEEALTFITAIKTMMSTFGKMMVTPVELHKRLNTKVWQAHTLAWDTIFK
  SVKPCIDNRLQRYSQQPGADFLCDIYQQDHLSKKELYAAVTELQLAAVETTANSLMWILYNLSRNPQAQR
  RLLQEVQSVLPDNQTPRAEDLRNMPYLKACLKESMRLTPSVPFTTRTLDKPTVLGEYALPKGTVLTLNTQ
  VLGSSEDNFEDSHKFRPERWLQKEKKINPFAHLPFGIGKRMCIGRRLAELQLHLALCWIIQKYDIVATDN
  EPVEMLHLGILVPSRELPIAFRPR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_201635
• ORF Size: 1542 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_201635.1, NM_201635.2, NM_201635.3, NP_963966.1
• RefSeq Size: 1545
• RefSeq ORF: 1545
• Locus ID: 25279
• MW: 59.4 kDa
• Gene Summary: enzyme involved in vitamin D metabolism; linked to albuminuria, hyperlipidemia and atherosclerotic aorta lesions [RGD, Feb 2006]